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A kind of acyltransferase and its application

An acyltransferase and enzymatic hydrolysis technology, applied in the field of enzyme engineering, can solve the problems of loss of neutral oil, long time consumption, etc., and achieve the effects of increasing the content of diglycerides and increasing economic benefits

Active Publication Date: 2021-07-30
SHANDONG YUWANG ECOLOGY FOOD IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that the enzyme-catalyzed degumming reaction takes too long, and because phospholipids are hydrolyzed by phospholipases into lysophospholipids and fatty acid salts, they are easily emulsified and cause neutral oil loss

Method used

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  • A kind of acyltransferase and its application
  • A kind of acyltransferase and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Heating soybean crude oil to 35°C, adding a certain mass concentration of 0.5% sodium hydroxide solution, adjusting the pH of soybean mixed oil to 7.5, and then adding acyltransferase (SEQ ID NO: 1) at a ratio of 100U / g mixed oil, Mix evenly, place the system in a closed constant temperature environment, set the stirring speed at 300r / min to carry out the enzyme reaction, and control the time at 1h; raise the temperature of the reaction to above 90°C to inactivate the enzyme for 15min. Crude oil enters the hydration degumming process after entering the 200-mesh scraper filter. Concentrated phospholipids are obtained after centrifugation and thin film evaporator. The soybean concentrated phospholipid prepared by this embodiment has improved transparency, and the content of diglyceride in soybean oil has increased by about 1%.

Embodiment 2

[0055] Heating soybean crude oil to 35°C, adding a certain mass of 0.5% sodium hydroxide solution, adjusting the pH of soybean mixed oil to 7.5, and then adding acyltransferase (SEQ ID NO: 1) at a ratio of 2000U / g mixed oil, Mix evenly, place the system in a closed constant temperature environment, set the stirring speed at 300r / min to carry out the enzyme reaction, and control the time at 1h; raise the temperature of the reaction to above 90°C to inactivate the enzyme for 15min. Crude oil enters the hydration degumming process after entering the 200-mesh scraper filter. Concentrated phospholipids are obtained after centrifugation and thin film evaporator. The soybean concentrated phospholipid prepared by this embodiment has obvious transparency improvement, and the content of diglyceride in soybean oil is increased by about 1.5%.

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Abstract

The invention provides an acyltransferase and its application, belonging to the technical field of enzyme engineering. The acyltransferase of the invention can be applied to the fields of phospholipid purification, oil refining and the like. Specifically, the acyltransferase can convert carboxyl groups in phospholipids into hydroxyl groups, eliminate the influence of phosphate calcium magnesium salts produced by non-hydratable phospholipids during the preparation of phospholipids, and make the prepared soybean concentrated phospholipids reach a transparent and stable state. In addition, the acyltransferase can increase the content of diglyceride in the product during the refining process of edible oil, thereby increasing the economic benefits of the oil industry.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to an acyltransferase and its application. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Vegetable oil is an indispensable food for human survival, and vegetable oil refining technology has also attracted much attention. With the development of vegetable oil industry and people's further understanding of the principle of vegetable oil processing, new progress has been made in vegetable oil refining technology. In 2001, Xu Zhenshan published "Analysis of Quality Restricting Factors of Domestic Concentrated Soybean Lecithin" in the "Grain and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12P7/64C11B3/00
CPCC11B3/00C12N9/1029C12P7/6481
Inventor 张恒明赵任飞房青辛寅昌
Owner SHANDONG YUWANG ECOLOGY FOOD IND
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