High-efficiency carbon or nitrogen supply device for microalgae autotrophy

A microalgae and high-efficiency technology, applied in enzymology/microbiology devices, biochemical cleaning devices, special-purpose bioreactors/fermenters, etc., can solve the high cost of heterotrophy/combination and environmental requirements High, loss and other problems, to achieve the effect of facilitating industrialization and application, increasing the density of microalgae culture, and promoting the growth of microalgae cells

Active Publication Date: 2020-01-14
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microalgae culture can be divided into photoautotrophic and non-photoautotrophic (heterotrophic / concurrent), among which non-photoautotrophic has the disadvantages of high equipment requirements, high environmental requirements, and expensive fermentation raw materials, which lead to heterotrophic / concurrent cultivation High and easily polluted
However, photoautotrophy encounters two bottlenecks in large-scale open systems: First, CO2 waste: CO2 is directly introduced into the runway pool, and the solubility of CO2 in water is extremely low, causing a large amount of CO2 to escape and cause serious losses; Second, adding directly in the form of carbonate or bicarbonate, as the anions (carbon) are utilized by the cells, the cations gradually accumulate, causing cell toxicity and inhibiting microalgae cultivation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-efficiency carbon or nitrogen supply device for microalgae autotrophy
  • High-efficiency carbon or nitrogen supply device for microalgae autotrophy
  • High-efficiency carbon or nitrogen supply device for microalgae autotrophy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The anion membrane and the bipolar membrane are selected and assembled into a cathode chamber and an anode chamber respectively, wherein the anode side of the bipolar membrane faces the microalgae chamber. The electrode selects stable inert electrode graphite, and the external power supply is supplied by alternating current through the Bio-Rad protein electrophoresis instrument. Sodium bicarbonate is added to the cathode chamber and sodium sulfate is added to the anode chamber. The graphite electrode is inserted into the chamber 3cm, and the microalgae chamber uses BG11-C or water as the medium to supply carbon for 2 hours by adjusting the current size of 4-10mA, and samples are taken every 20 minutes to detect the content of sodium ions and the amount of carbon supplied. Among them, the sodium ion is determined by the TP330 sodium ion detector for detection, and the carbon determination method adopts the institute's TIC determination method, NO 3 - The determination ...

Embodiment 2

[0044] The anion membrane and the bipolar membrane are selected and assembled into a cathode chamber and an anode chamber respectively, wherein the anode side of the bipolar membrane faces the microalgae chamber. The electrode selects stable inert electrode graphite, and the external power supply is supplied by alternating current through the Bio-Rad protein electrophoresis instrument. Sodium nitrate is added to the cathode chamber and sodium sulfate is added to the anode chamber. The graphite electrode is inserted into the chamber 3cm, and the microalgae chamber uses BG11-N or water as the medium to supply carbon for 2 hours by adjusting the current size of 4-10mA, and samples are taken every 20 minutes to detect the sodium ion content and carbon supply. Among them, the sodium ion is determined by the TP330 sodium ion detector for detection, and the carbon determination method adopts the institute's TIC determination method, NO 3 - The determination method adopts spectropho...

Embodiment 3

[0047] The anion membrane and the bipolar membrane are selected and assembled into a cathode chamber and an anode chamber respectively, wherein the anode side of the bipolar membrane faces the microalgae chamber. The electrode selects stable inert electrode graphite, and the external power supply is supplied by alternating current through the Bio-Rad protein electrophoresis instrument. Sodium bicarbonate is added to the cathode chamber and sodium sulfate is added to the anode chamber. Graphite electrodes are inserted into the chamber for 5cm, and after 3-10 minutes of power-on, the power supply current is controlled at 4-1000mA. The microalgae chamber uses BG11-C or water as the medium, and the microalgae to be cultured and the culture solution are put into the self-supporting microalgae. Self-supporting in high-efficiency carbon or nitrogen supply devices; real-time monitoring of the pH value of the microalgae chamber. If the pH value of the microalgae chamber is lower than 6...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a high-efficiency carbon or nitrogen supply device for microalgae autotrophy. The high-efficiency carbon or nitrogen supply device comprises a reaction chamber, the reaction chamber has a certain accommodating cavity, the reaction chamber is provided with an anion membrane and a bipolar membrane, three independent sub-chambers are formed in the reaction chamber, thesub-chamber between the anion membrane and the bipolar membrane is a microalgae chamber, microalgae to be cultivated and a culture solution are placed in the microalgae chamber, the sub-chamber on the other side of the anion membrane is an cathode chamber, a bicarbonate solution or a nitrate solution is added to the cathode chamber, only anions are allowed to enter the microalgae chamber from thecathode chamber, the sub-chamber on the other side of the polar membrane is an anode chamber, an anode surface of the bipolar membrane faces the microalgae chamber, a sulfate solution is added to theanode chamber, and only cations are allowed to enter the microalgae chamber from the anode chamber side and anions from the microalgae chamber side enter the anode chamber; and inert electrodes are respectively arranged in the cathode chamber and the anode chamber and are also connected with an external power supply. The high-efficiency carbon or nitrogen supply device can effectively isolate thecations outside the microalgae chamber and exclusively supply the anions (HCO3- / NO3-) for the microalgae autotrophy.

Description

technical field [0001] The invention relates to the field of microbial self-supporting equipment. More specifically, the present invention relates to a high-efficiency carbon or nitrogen supply device for microalgae autotrophy. Background technique [0002] Microalgae (microalgae) refers to those tiny groups of algae whose shape can only be distinguished under a microscope, and is a general term for microorganisms that have chlorophyll a and can perform photosynthesis. There are many kinds of microalgae, and microalgae cells contain high-value nutrients and chemical raw materials such as protein, lipid, algal polysaccharide, β-carotene, and various inorganic elements (such as Cu, Fe, Se, Mn, Zn, etc.) ; Its protein content is very high, and it is an important source of single-cell protein (SCP); vitamin A, vitamin E, thiamine, riboflavin, pyridoxine, vitamin B12, vitamin C, biotin contained in microalgae , inositol, folic acid, calcium pantothenate, and niacin, among other...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M23/34
Inventor 侯余勇陈方见刘智永陈树林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap