A device and method for continuous whole enzymatic synthesis of diglycerides in a multi-liquid phase system

A technology for diglyceride and enzymatic synthesis, which is applied in sterilization methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of high cost, complicated reaction operation, difficult continuous production, etc. Material consumption, simplification of process flow, beneficial effect of extraction and separation

Active Publication Date: 2022-02-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a continuous full enzymatic synthesis of diacylglycerol in a multi-liquid phase system in view of the problems of high cost, complex reaction operation and difficulty in continuous production in the process of preparing diacylglycerol from existing oil plants. Apparatus and methods for esters

Method used

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  • A device and method for continuous whole enzymatic synthesis of diglycerides in a multi-liquid phase system

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] like figure 1 As shown, a multi-liquid phase system continuous enzymatic synthesis of diglycerides, including a hydrolysis device and an esterification device, the specific structure is as follows:

[0052] The hydrolysis device includes a device reaction zone 1, a dispersion buffer zone 2, a static zone 3, a first stirrer 4, an automatic potentiometric titrator burette 7, an automatic potentiometric titrator electrode head 13 and a first circulating water heating sleeve 14. Between the device reaction zone 1 and the dispersion buffer zone 2, there is a baffle plate 10 filled with several small holes, and between the device reaction zone 1 and the resting area 3, there is also a baffle plate 12 covered with several small holes. An oil sample inlet 17 and a lower phase solution outlet 18 are arranged at the lower part of the dispersion buffer zone 2 . In the standing area 3, the upper, middle and lower three liquid phase areas are divided. The upper liquid phase area is...

Embodiment 2

[0060] The method for continuously synthesizing diglycerides using the device of embodiment 1 may further comprise the steps:

[0061] Step (1): First prepare a 3L aqueous two-phase system, that is, according to the mass fraction of 20% (NH 4 ) 2 SO 4 , Dissolve 20% PEG600 and 60% water and mix well to prepare in a 3L separatory funnel. Place the separatory funnel in an incubator at 37°C for 24 hours. After being clearly separated, the two-phase solution was passed through a 0.45 μm filter membrane to remove impurities to obtain the upper phase PEG600 and the lower phase (NH 4 ) 2 SO 4 solution.

[0062] Step (2): Turn on the first circulating water heat pump 27, add 5g Lipase AY30 enzyme and 10mL [BMIM]BF to 100mL upper phase PEG600 4 , 300mL lower phase Na 2 SO 4 The solution is passed into the static zone 3 under the condition of heating and stirring to make it full of the dispersion buffer 2, and then the PEG600 solution containing enzyme is passed into the reactio...

Embodiment 3

[0066] The method for continuously synthesizing diglycerides using the device of embodiment 1 may further comprise the steps:

[0067] Step (1): First prepare a 3L aqueous two-phase system, that is, according to the mass fraction of 20% PEG600, 15% Na 2 SO 4 , 65% of the water was dissolved and mixed evenly for preparation in a 3L separatory funnel, and the separatory funnel was placed in an incubator at 37°C for 24 hours. After clear separation, the two-phase solution was passed through a 0.45 μm filter membrane to remove impurities to obtain the upper phase PEG600 and the lower phase Na 2 SO 4 solution.

[0068] Step (2): Turn on the first circulating water heat pump 27, and add 7.5g LipaseTL100L enzyme into 150mL upper phase PEG600. 300mL lower phase Na 2 SO 4 Under the conditions of heating and stirring, pass it into the static zone 3 to make it full of the dispersion buffer 2, and then pass the enzyme-containing PEG600 solution into the reaction zone 1, the stirring...

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Abstract

The invention belongs to the field of bioengineering and food technology, and discloses a device and method for continuously enzymatically synthesizing diglycerides in a multi-liquid phase system, mainly including a hydrolysis device and an esterification device, wherein the hydrolysis device is divided into two parts from top to bottom: Quiet zone, reaction zone and dispersion buffer. The reaction zone and the static zone and between the reaction zone and the dispersion buffer zone are separated by baffles, and there are a number of small holes connecting each zone on the baffle; There is a liquid outlet for hydrolyzate, which is connected with the inlet of the esterification device, and the fatty acid is directly passed through to synthesize diglyceride by enzymatic method. The middle liquid phase area is connected with the reaction area through the return pipe, and the lower liquid phase area is There is a lower-phase solution inlet; the lower part of the reaction zone is equipped with an enzyme-containing solution inlet, and there is a stirrer inside; the bottom of the dispersion buffer is equipped with an oil sample inlet and a lower-phase solution outlet. The invention has the advantages of high recycling rate of raw materials, mild and controllable reaction conditions, continuous production and the like.

Description

technical field [0001] The invention belongs to the field of bioengineering and food technology, relates to the fields of oil industry and oil chemical industry, and particularly relates to a device and method for continuously enzymatically synthesizing diglyceride. Background technique [0002] Diacylglycerol is the esterification product of two hydroxyl groups on glycerol and two fatty acids, and has two isomers, 1,2-DAG and 1,3-DAG. The content of diglyceride in natural animal and vegetable oils is very small. For example, olive oil contains 5.5% diglyceride. Its preparation is mainly completed by oil modification technology, mainly chemical method and enzymatic method. The chemical method has complex process and energy consumption. However, the enzymatic method has attracted much attention because of its high efficiency, mildness, specificity and environmental friendliness. Enzyme methods mainly include oil hydrolysis, direct esterification and glycerol hydrolysis. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/40C12M1/34C12M1/02C12M1/00C12P7/6454
CPCC12M21/18C12M23/34C12M23/58C12M27/02C12M29/18C12M29/00C12M33/04C12M37/04C12M41/22C12M41/26C12P7/6454Y02P20/54
Inventor 李志刚杨嘉威杨博王永华陈华勇王卫飞
Owner SOUTH CHINA UNIV OF TECH
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