Efficient carbon or nitrogen supply device for microalgae autotrophy

A microalgae, high-efficiency technology, used in enzymology/microbiology devices, biochemical cleaning devices, specific-purpose bioreactors/fermenters, etc. Low solubility and other problems, to achieve the effect of facilitating industrialization and application, promoting the growth of microalgae cells, and increasing the culture density

Active Publication Date: 2022-04-22
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microalgae culture can be divided into photoautotrophic and non-photoautotrophic (heterotrophic / concurrent), among which non-photoautotrophic has the disadvantages of high equipment requirements, high environmental requirements, and expensive fermentation raw materials, which lead to heterotrophic / concurrent cultivation High and easily polluted
However, photoautotrophy encounters two bottlenecks in large-scale open systems: First, CO2 waste: CO2 is directly introduced into the runway pool, and the solubility of CO2 in water is extremely low, causing a large amount of CO2 to escape and cause serious losses; Second, adding directly in the form of carbonate or bicarbonate, as the anions (carbon) are utilized by the cells, the cations gradually accumulate, causing cell toxicity and inhibiting microalgae cultivation

Method used

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  • Efficient carbon or nitrogen supply device for microalgae autotrophy
  • Efficient carbon or nitrogen supply device for microalgae autotrophy
  • Efficient carbon or nitrogen supply device for microalgae autotrophy

Examples

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Effect test

Embodiment 1

[0040] The anion membrane and the bipolar membrane are selected and assembled into a cathode chamber and an anode chamber respectively, wherein the anode side of the bipolar membrane faces the microalgae chamber. The electrode selects stable inert electrode graphite, and the external power supply is supplied by alternating current through the Bio-Rad protein electrophoresis instrument. Sodium bicarbonate is added to the cathode chamber and sodium sulfate is added to the anode chamber. The graphite electrode is inserted into the chamber 3cm, and the microalgae chamber uses BG11-C or water as the medium to supply carbon for 2 hours by adjusting the current size of 4-10mA, and samples are taken every 20 minutes to detect the content of sodium ions and the amount of carbon supplied. Among them, the sodium ion is determined by the TP330 sodium ion detector for detection, and the carbon determination method adopts the institute's TIC determination method, NO 3 - The determination ...

Embodiment 2

[0044] The anion membrane and the bipolar membrane are selected and assembled into a cathode chamber and an anode chamber respectively, wherein the anode side of the bipolar membrane faces the microalgae chamber. The electrode selects stable inert electrode graphite, and the external power supply is supplied by alternating current through the Bio-Rad protein electrophoresis instrument. Sodium nitrate is added to the cathode chamber and sodium sulfate is added to the anode chamber. The graphite electrode is inserted into the chamber 3cm, and the microalgae chamber uses BG11-N or water as the medium to supply carbon for 2 hours by adjusting the current size of 4-10mA, and samples are taken every 20 minutes to detect the sodium ion content and carbon supply. Among them, the sodium ion is determined by the TP330 sodium ion detector for detection, and the carbon determination method adopts the institute's TIC determination method, NO 3 - The determination method adopts spectropho...

Embodiment 3

[0047] The anion membrane and the bipolar membrane are selected and assembled into a cathode chamber and an anode chamber respectively, wherein the anode side of the bipolar membrane faces the microalgae chamber. The electrode selects stable inert electrode graphite, and the external power supply is supplied by alternating current through the Bio-Rad protein electrophoresis instrument. Sodium bicarbonate is added to the cathode chamber and sodium sulfate is added to the anode chamber. Graphite electrodes are inserted into the chamber for 5cm, and after 3-10 minutes of power-on, the power supply current is controlled at 4-1000mA. The microalgae chamber uses BG11-C or water as the medium, and the microalgae to be cultured and the culture solution are put into the self-supporting microalgae. Self-supporting in high-efficiency carbon or nitrogen supply devices; real-time monitoring of the pH value of the microalgae chamber. If the pH value of the microalgae chamber is lower than 6...

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Abstract

The invention discloses a high-efficiency carbon or nitrogen supply device for microalgae autotrophy, comprising: a reaction chamber, which has a certain accommodation cavity, and an anion membrane and a bipolar membrane are arranged in the reaction chamber to form three independent The sub-chamber, wherein the sub-chamber between the anion membrane and the bipolar membrane is the microalgae chamber, in which the microalgae and the culture solution to be raised are placed, and the subchamber on the other side of the anion membrane is the cathode chamber, and in the cathode chamber Adding bicarbonate solution or nitrate solution only allows anions to enter the microalgae chamber from the cathode chamber, the other side of the bipolar membrane is the anode chamber, the anode of the bipolar membrane faces the microalgae chamber, and the Sulfate solution only allows cations to enter the microalgae chamber from the anode chamber side, and anions enter the anode chamber from the microalgae chamber side; inert electrodes are respectively arranged in the cathode chamber and the anode chamber, and are connected to an external power supply. The present invention can effectively isolate cations outside the microalgae and supply anions (HCO 3 ‑ / NO 3 ‑ ) are autotrophic in microalgae.

Description

technical field [0001] The invention relates to the field of microbial self-supporting equipment. More specifically, the present invention relates to a high-efficiency carbon or nitrogen supply device for microalgae autotrophy. Background technique [0002] Microalgae (microalgae) refers to those tiny groups of algae whose shape can only be distinguished under a microscope, and is a general term for microorganisms that have chlorophyll a and can perform photosynthesis. There are many kinds of microalgae, and microalgae cells contain high-value nutrients and chemical raw materials such as protein, lipid, algal polysaccharide, β-carotene, and various inorganic elements (such as Cu, Fe, Se, Mn, Zn, etc.) ; Its protein content is very high, and it is an important source of single-cell protein (SCP); vitamin A, vitamin E, thiamine, riboflavin, pyridoxine, vitamin B12, vitamin C, biotin contained in microalgae , inositol, folic acid, calcium pantothenate, and niacin, among other...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M23/34
Inventor 侯余勇陈方见刘智永陈树林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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