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Enrichment method of anaerobic ammonia-oxidizing bacteria

An anammox bacteria and enrichment technology, applied in the field of environmental microorganisms, can solve the problems of inability to simulate normal wastewater conditions, single nutrient solution components, and inability to coordinate with target strains, and achieve elimination of organic pollutants in water bodies and strong COD removal ability. , Good adaptation to the effect of wastewater environment

Active Publication Date: 2020-01-14
WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned enrichment method has the following disadvantages: 1) using artificially configured chemical agents as the nutrient solution of anammox bacteria, when enriching and culturing, the nutrient solution has a single component, which cannot simulate the normal wastewater situation; 2) simply using wastewater as nutrient solution Because there are many types of microorganisms in the wastewater, it is impossible to accurately control the ratio of ammonia nitrogen and nitrite in the effluent, which makes it difficult to meet the nutritional needs of the target strains; 3) The types and quantities of microorganisms contained in the nutrient solution are not as many as the target wastewater. The target strains cannot be truly synergistic and domesticated, resulting in the rejection of the enriched target strains in the subsequent application process, resulting in system collapse; 4) The enrichment effect and total nitrogen removal rate have not been greatly improved improvement, can not meet the industrial application

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Examples

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Effect test

Embodiment 1

[0047] The enrichment method of anammox bacteria in municipal wastewater specifically comprises the following steps:

[0048] S1. Wastewater pretreatment: put anaerobic enhanced bacteria with a volume concentration of 0.01% into municipal wastewater, and perform anaerobic reaction for 6 hours;

[0049] S2. Aeration treatment: anaerobic effluent enters the aeration device, and the aeration device adds aerobic enhanced bacteria with a volume concentration of 0.01% and autotrophic nitrifying bacteria with a volume concentration of 0.01% to degrade wastewater COD and ammonia nitrogen. The reaction time of aeration treatment is 24 hours, to ensure that the effluent COD is less than 100mg / L, and the ratio of ammonia nitrogen to nitrite is about 1:1;

[0050] S3. Enrichment treatment: the effluent of the aeration device enters the enrichment device, and the influent water is left to react for 4 hours, and the water is ingested 6 times a day; the long-term operation until the appearan...

Embodiment 2

[0052] The method for enriching anammox bacteria in aquaculture wastewater specifically includes the following steps:

[0053] S1. Wastewater pretreatment: Put the anaerobic enhanced bacteria with a volume concentration of 0.02% into the culture wastewater that has undergone solid-liquid separation, and perform anaerobic reaction for 12 hours; Azotobacter, react for 12h;

[0054] S2. Aeration treatment: anaerobic effluent enters the aeration device, and the aeration device adds aerobic enhanced bacteria with a volume concentration of 0.02% and autotrophic nitrifying bacteria with a volume concentration of 0.02% to degrade wastewater COD and ammonia nitrogen. The reaction time of aeration treatment is 12 hours, to ensure that the effluent COD is less than 100mg / L, and the ratio of ammonia nitrogen to nitrite is 1:1;

[0055] S3. Enrichment treatment: the effluent of the aeration device enters the enrichment device, and the influent water is left to react for 4 hours, and the w...

Embodiment 3

[0057] The method for enriching anammox bacteria in landfill leachate specifically includes the following steps:

[0058] S1. Wastewater pretreatment: put anaerobic enhanced bacteria with a volume concentration of 0.04% into the landfill leachate, and perform anaerobic reaction for 36 hours;

[0059] S2. Aeration treatment: anaerobic effluent enters the aeration device, and the aeration device adds aerobic enhanced bacteria with a volume concentration of 0.04% and autotrophic nitrifying bacteria with a volume concentration of 0.04% to degrade wastewater COD and ammonia nitrogen. The reaction time of aeration treatment is 72 hours, to ensure that the effluent COD is less than 100mg / L, and the ratio of ammonia nitrogen to nitrite is 1:1;

[0060] S3. Enrichment treatment: the effluent of the aeration device enters the enrichment device, and the influent water is left to react for 4 hours, and the water is ingested 6 times a day; long-term operation until the appearance of red ba...

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Abstract

The invention provides an enrichment method of anaerobic ammonia-oxidizing bacteria. According to the method, wastewater is used as an enrichment substrate and a nutrient source, and a microbial agentis added to treat the wastewater in different stages. On the one hand, the required enrichment culture conditions for anaerobic ammonia-oxidizing bacteria can be provided, so that outlet water COD and ammonia nitrogen nitrites reach requirements for enrichment of an anaerobic ammonia-oxidizing strain; and on the other hand, the synergistic domestication action of the target strain is performed during the enrichment process, the growth rate and tolerance performance of the anaerobic ammonia-oxidizing bacteria can be improved, and the enrichment effect and the total nitrogen removal rate of theanaerobic ammonia-oxidizing bacteria are improved. Quick start of the strain and an apparatus can be ensured in a subsequent application process, and thereby a process of biological nitrogen removalby an anaerobic ammonia-oxidizing process is accelerated.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a method for enriching anammox bacteria. Background technique [0002] Anammox bacteria is a kind of chemoautotrophic bacteria, which can denitrify without aeration and without external carbon source. It is of great significance to the global nitrogen cycle and is also an important bacterium in sewage treatment. Anammox reaction refers to the biological process in which anammox bacteria use NO2--N as electron acceptor to oxidize NH4+-N to nitrogen under anaerobic or anoxic conditions. Compared with traditional nitrification-reaction Nitrification process, which can reduce 50% aeration, 100% organic carbon source and 90% operating cost, and the sludge yield is low. Anammox process has become a research hotspot in the field of wastewater biological denitrification because of its outstanding advantages. [0003] However, there are many difficulties...

Claims

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Application Information

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IPC IPC(8): C02F3/30C02F3/34C02F101/16C02F101/30
CPCC02F3/30C02F3/34C02F2101/16C02F2101/30
Inventor 廖东栋王璐琛虞滨荆峰洋陈维
Owner WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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