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Method for extracting ergosterol from distillers'grains

A technology of ergosterol and extraction method, which is applied in the field of herbal medicine, can solve the problems that the output of ergosterol cannot keep up with the demand, and achieve the effect of saving cost and benefiting the environment

Active Publication Date: 2020-01-10
宿迁医美科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention extracts ergosterol from distiller's grains, and the method can solve the problem of disposing of waste distiller's grains, and alleviate the current situation that the production of ergosterol cannot keep up with the demand

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  • Method for extracting ergosterol from distillers'grains
  • Method for extracting ergosterol from distillers'grains
  • Method for extracting ergosterol from distillers'grains

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Embodiment 1

[0029] A method for extracting ergosterol in distiller's grains, said extraction method comprising the steps of:

[0030] Saponification: 100g of dried distiller's grains was heated and extracted twice with 800mL of 95% ethanol and 80mL of saturated potassium hydroxide solution under reflux, each time for 2h, and the extracts were combined;

[0031] Extraction: first concentrate the extract 4 times under reduced pressure, then extract 3 times with 660mL n-hexane, combine the n-hexane layers, and concentrate the n-hexane layer under reduced pressure to obtain 2.5125g of the extraction product of a yellow solid, and reclaim the n-hexane, the extraction product TLC And HPLC analysis, wherein: TLC condition is: developing agent is that the volume ratio of sherwood oil and ethyl acetate is 3:1; HPLC condition is: chromatographic column is normal phase chromatographic column Inertsil, mobile phase is the mixture of normal hexane and isopropanol The volume ratio is 100:1, the flow ra...

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Abstract

The invention relates to a method for extracting ergosterol from distillers'grains, and belongs to the field of botanical drugs. The extraction method comprises the following steps: extracting distillers'grains by using an ethanol-water solution and a potassium hydroxide solution, extracting the obtained extract solution with n-hexane, and separating the obtained extract product by using a silicagel column to obtain ergosterol. Existing industrially extracted ergosterol is directly obtained by extraction from related fungi, leaching and recrystallization. The ergosterol is extracted from distillers' grains, so that the method is beneficial to the environment and saves the cost.

Description

technical field [0001] The invention relates to a method for extracting ergosterol in distiller's grains, which belongs to the field of herbal medicines. Background technique [0002] "Ergosterol", also known as "ergosterol", is one of the steroid compounds. This compound can be used in biochemical research, and some of its effects are the same as vitamin D2. Ergosterol is an important part of the microbial cell membrane, which plays an important role in ensuring the integrity of the cell membrane, the activity of membrane-bound enzymes, the fluidity of the membrane, cell viability, and the transport of cellular substances. It is the precursor for the production of vitamin D2 and is also It is an intermediate in the production of hormone drugs and can be used to produce cortisone. Ergosterol is a plant sterol found in the fungi yeast and ergot, which can be converted into vitamin D2 under ultraviolet light. Studies have shown that ergosterol may have anti-tumor properties,...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 胡晓进彭缨
Owner 宿迁医美科技有限公司
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