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Sericin/nano-hydroxyapatite tissue engineering bone graft as well as preparation method and application thereof

A nano-hydroxyapatite and sericin technology, applied in bone implants, tissue regeneration, prostheses, etc., can solve problems such as brittleness constraints, nHAP refractory degradation, and difficulty in meeting requirements, achieving high porosity, Good biocompatibility, overcome the effect of direct application

Active Publication Date: 2020-01-10
赣南医学院第一附属医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, a single artificial material is generally difficult to meet the requirements of extracellular scaffold materials for bone tissue engineering, and the refractory and brittle nature of nHAP restricts its clinical use.

Method used

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  • Sericin/nano-hydroxyapatite tissue engineering bone graft as well as preparation method and application thereof
  • Sericin/nano-hydroxyapatite tissue engineering bone graft as well as preparation method and application thereof
  • Sericin/nano-hydroxyapatite tissue engineering bone graft as well as preparation method and application thereof

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Embodiment approach

[0058] According to a preferred embodiment of the present invention, the pore diameter of the nano-hydroxyapatite is 100-250 μm, the porosity is above 90%, and the pores are interconnected pores.

[0059] According to a preferred embodiment of the present invention, the molecular weight of the sericin is 50kDa-250kDa.

[0060] According to a preferred embodiment of the present invention, the sericin protein is sericin protein with undamaged natural structure extracted from silkworm lacking mutant silkworm 185N-ds silkworm eggs.

[0061] According to a preferred embodiment of the present invention, the cross-linking agent is glutaraldehyde, genipin, horseradish peroxidase and H 2 o 2 A kind of the mixture of, preferably described cross-linking agent is the mixture of horseradish peroxidase and H2O2.

[0062] According to a preferred embodiment of the present invention, the growth factor may be conventionally selected in the field, for example, the growth factor is BMP2, prefe...

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Abstract

The invention belongs to the technical field of tissue engineering, and discloses a construction (preparation) method of a sericin / nano-hydroxyapatite tissue engineering bone graft containing bone morphogenetic protein-2 and an application thereof. The graft comprises growth factors, a scaffold material and optional seed cells, wherein the seed cells are adhered to the scaffold material, a cell carrier compound with a space structure and biological activity is formed, the scaffold material is a composite scaffold material composed of sericin (SS) and nano-hydroxyapatite (nHAP), the sericin wraps a growth factor containing human recombinant bone morphogenetic protein-2, and bone marrow mesenchymal stem cells are adhered to the composite scaffold material. The pore diameter, degradation speed and biomechanical strength of the scaffold can be adjusted according to the ratio of SS to nHAP. The constructed bionic artificial tissue engineering bone can be used for repairing bone grafts withlarge-section bone defects, and animal experiments prove that the bionic artificial tissue engineering bone can well repair the large-section bone defects.

Description

technical field [0001] The invention relates to a biodegradable active material for repairing bone defects, belonging to the technical field of tissue engineering, in particular to the construction of a bionic artificial bone and its application in orthopedics, more specifically to sericin / nano hydroxyapatite Stone tissue engineered bone graft and its preparation method and application. Background technique [0002] Bone tissue defect caused by trauma, tumor, congenital deformity, infection, pathology and other factors is one of the clinical problems, and bone grafting is the main method to solve this problem. Bone grafting is mainly divided into autologous bone grafting, allogeneic or heterogeneous bone grafting. The drawbacks or limitations of these two methods mainly include insufficient supply sources, donor site damage, complications after bone harvesting, and graft rejection. In recent years, with the development of tissue engineering, tissue engineering bone grafts ...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/22A61L27/50A61L27/56A61L27/58A61F2/28
CPCA61L27/56A61L27/12A61L27/227A61L27/58A61L27/50A61F2/28A61L2300/252A61L2300/414A61L2300/602A61L2430/02A61F2002/2835A61F2310/00371A61F2310/00293
Inventor 何春耒张立兵张业顺
Owner 赣南医学院第一附属医院
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