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Method for separating and purifying mixed-bean pectic polysaccharides

A pectin polysaccharide, separation and purification technology can solve the problems of low separation efficiency, low extraction efficiency, low efficiency, etc.

Active Publication Date: 2020-01-03
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, comparing the current research on miscellaneous polysaccharides, it is not difficult to find that: (1) miscellaneous polysaccharides are rich in acidic polysaccharides, and their biological activities, especially hypoglycemic activity, have been widely concerned; (2) there are few studies on the extraction process of miscellaneous polysaccharides, The extraction efficiency is low; (3) the separation and purification of miscellaneous bean polysaccharides, such as phase bean polysaccharides, chickpea polysaccharides and pea polysaccharides, etc. are mainly based on the negative filler DEAE Sepharose Fast Flow, which has cumbersome elution steps, high cost and low efficiency. It is unfavorable for industrialized production; (4) the inventors have found that there is no special research on the extraction and separation and purification technology of multiple miscellaneous bean polysaccharides at the same time, and the establishment of a separation and purification model for polysaccharides in this class of miscellaneous beans
In summary, the problems concerning the extraction and separation and purification of polysaccharides from miscellaneous beans are concentrated on low separation efficiency, complex separation methods, and difficulty in industrial production, and there is no relevant research on effective separation and purification methods for polysaccharides such as miscellaneous beans.

Method used

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  • Method for separating and purifying mixed-bean pectic polysaccharides
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  • Method for separating and purifying mixed-bean pectic polysaccharides

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Red bean polysaccharide extraction:

[0040] Process 1000g red bean raw material by ultrafine grinding, collect the fine powder passed through a 250 mesh sieve, add 10L volume concentration ratio of 95% ethanol to soak for 24 hours to degrease, filter and dry, and extract the dried red bean powder with hot water at 95°C for 2 hours twice , combine the extracts, add α-amylase, protease, and glucoamylase in turn for enzymolysis, the enzymolysis conditions are α-amylase at a temperature of 95°C, time 1.5h, protease at a temperature of 60°C, time 0.5h, pH 4.5 , glucoamylase at 60°C, time 0.5h, amylase hydrolyzes starch into dextrin, glucoamylase hydrolyzes dextrin into glucose, protease hydrolyzes protein into polypeptide, inactivates the enzyme at 100°C for 10 minutes, and then Centrifuge at a speed of 8000r / min for 10min, take the supernatant and concentrate it in vacuum, then add 95% ethanol 5.33 times the volume of the concentrated solution, let it stand at 4°C, red...

Embodiment 2

[0050] (1) White lentil polysaccharide extraction:

[0051] Process 1000g of white lentil raw material by ultrafine grinding, collect the fine powder passed through a 250-mesh sieve, add 10 L of ethanol with a volume concentration ratio of 95% to soak for 24 hours to degrease, filter and dry, and extract the dried white lentil powder with hot water at 95°C for 2 hours twice , combine the extracts, add α-amylase, protease, and glucoamylase in turn for enzymolysis, the enzymolysis conditions are α-amylase at a temperature of 95°C, time 1.5h, protease at a temperature of 60°C, time 0.5h, pH 4.5 , glucoamylase at 60°C, time 0.5h, amylase hydrolyzes starch into dextrin, glucoamylase hydrolyzes dextrin into glucose, protease hydrolyzes protein into polypeptide, inactivates the enzyme at 100°C for 10 minutes, and then Centrifuge at a speed of 8000r / min for 10min, take the supernatant and concentrate it in a vacuum, then add 95% ethanol 5.33 times the volume of the concentrated soluti...

Embodiment 3

[0061] (1) Red kidney bean polysaccharide extraction:

[0062] Process 1000g of red kidney bean raw material by ultrafine grinding, collect the fine powder passed through a 250-mesh sieve, add 10 L of ethanol with a volume concentration ratio of 95% to soak for 24 hours to degrease, filter and dry, and extract the dried red kidney bean powder with hot water at 95°C for 2 hours twice , combine the extracts, add α-amylase, protease, and glucoamylase in turn for enzymolysis, the enzymolysis conditions are α-amylase at a temperature of 95°C, time 1.5h, protease at a temperature of 60°C, time 0.5h, pH 4.5 , glucoamylase at 60°C, time 0.5h, amylase hydrolyzes starch into dextrin, glucoamylase hydrolyzes dextrin into glucose, protease hydrolyzes protein into polypeptide, inactivates the enzyme at 100°C for 10 minutes, and then Centrifuge at 8000r / min for 10min, take the supernatant and concentrate it in vacuum, then add 95% ethanol 5.33 times the volume of the concentrated solution, ...

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Abstract

The invention discloses a method for separating and purifying mixed-bean pectic polysaccharides. According to the method, three different species of mixed beans comprising small red beans, white hyacinth beans and red kidney beans are used as raw materials. Through superfine grinding, hot water extraction, homogeneous-pore strongly-acidic styrene filler column adsorption and ethanol precipitation,mixed-bean refined polysaccharides with a pectic polysaccharide content of about 90% are quickly and efficiently prepared from the mixed beans, and gel column separation and purification are carriedout on the mixed-bean refined polysaccharides to finally prepare the mixed-bean pectic polysaccharides with good uniformity. The whole method is rapid and is good in stability from extraction to separation, and the obtained mixed-bean pectic polysaccharides are high in purity and good in quality.

Description

technical field [0001] The invention relates to the technical field of separation and purification of plant polysaccharides, in particular to a method for separation and purification of pectin polysaccharides from miscellaneous beans. Background technique [0002] Miscellaneous beans (Pulses) refers to the general term for small starchy beans other than soybeans, mainly including peas, broad beans, red beans, lentils, cowpeas, kidney beans, chickpeas, etc., which have high protein, low fat, low Allergenicity and other characteristics, and the price is low. The "Dietary Guidelines for Chinese Residents (2016)" advocates whole grains, miscellaneous beans and potatoes as an important part of the diet, and recommends a daily intake of 50-150g of whole grains and miscellaneous beans. China is a big producer of miscellaneous beans, which are rich in miscellaneous bean resources. However, the low utilization rate causes serious waste of miscellaneous bean resources. In addition, t...

Claims

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Application Information

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IPC IPC(8): C08B37/06
CPCC08B37/0048
Inventor 聂少平刘丹唐炜白周亚李安琪杨靖睿辛玥黄晓君殷军艺
Owner NANCHANG UNIV
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