Method for improving photosynthetic efficiency of plants

A photosynthetic efficiency, plant technology, applied in botany equipment and methods, biochemical equipment and methods, plant peptides, etc., can solve the problem of drought tolerance reduction and other issues

Active Publication Date: 2019-12-31
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, our study found that plants overexpressing GDH and MS genes in chloroplasts, while inhibiting the expression of PLGG1 gene, had significantly reduced drought tolerance compared with the control

Method used

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  • Method for improving photosynthetic efficiency of plants
  • Method for improving photosynthetic efficiency of plants
  • Method for improving photosynthetic efficiency of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the construction of carrier

[0036] The GDH genes of the present invention can be derived from prokaryotes or eukaryotes. The GDH genes provided by the present invention include but are not limited to the genes shown in Table 2. In order to construct the transformation vector, the E. coli-derived GDH gene and the corresponding terminator sequence were artificially synthesized, including the chloroplast signal peptide, the GDH coding gene and the terminator. The nucleotide sequence is shown in SEQID NO. ' ends are provided with BamHI and KpnI sites, respectively. The artificially synthesized GCL gene includes a chloroplast signal peptide, a GCL coding gene and a terminator, the nucleotide sequence is shown in SEQ ID NO. 8, and the 5' end and the 3' end are respectively provided with BamHI and HindIII sites. The artificially synthesized TSR gene includes a chloroplast signal peptide, a TSR coding gene and a terminator, the nucleotide sequence is shown in S...

Embodiment 2

[0043] Example 2, rice transformation

[0044] The method of obtaining transgenic rice is to use the existing technology (Lu Xiongbin, Gong Zuxun (1998) Life Science 10: 125-131; Liu Fan et al. (2003) Molecular Plant Breeding 1: 108-115). The mature and plump "Xiushui-134" seeds were selected and shelled to induce callus as the transformation material. Take the Agrobacterium plates constructed in Example 1 containing the plasmids pCambia1300-bar-GDH-GCL-TSR-OsBASS-RNAi (GGTOsBi) and pCambia1300-bar-GDH-GCL-TSR-OsPGGL1-RNAi (GGTOsPi) respectively. Pick a single colony to inoculate and prepare for transformation with Agrobacterium. The callus to be transformed is put into the Agrobacterium liquid with an OD of about 0.6 (the preparation of the Agrobacterium liquid: the Agrobacterium is inoculated into the medium, and cultivated to an OD of about 0.6; the medium consists of 3 g / L K 2 HPO 4 , 1g / L NaH 2 PO 4 , 1g / L NH 4 Cl, 0.3g / L MgSO 4 ·7H 2 O, 0.15g / L KCl, 0.01g / L CaCl ...

Embodiment 3

[0045] Example 3. Soybean Transformation

[0046] The steps used here to obtain transgenic soybeans come from existing technologies (Deng et al., 1998, PlantPhysiology Communications 34:381-387; Ma et al., 2008, Scientia Agriculture Sinica 41:661-668; Zhou et al., 2001 , Journal of Northeast Agricultural University 32:313-319). Select healthy, plump, and mature "Tianlong No. 1" soybeans, disinfect them with 80% ethanol for 2 minutes, wash them with sterile water, and place them in a desiccator filled with chlorine gas (generated by the reaction of 50ml NaClO and 2ml concentrated HCl) Sterilize for 4-6 hours. The sterilized soybeans were sowed into the B5 medium in the ultra-clean workbench, cultured at 25°C for 5 days, and the optical density was at 90-150μmol photons / m 2 · s level. When the cotyledons turn green and break through the seed coat, sterile bean sprouts will grow. The bean sprouts from which the hypocotyls were removed were cut 50-50 in length so that both exp...

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Abstract

The invention discloses a method for improving photosynthetic efficiency of plants, which comprises the following steps: inhibiting or knocking out bile acid sodium cotransporter genes in the plants,and simultaneously over-expressing glycollate dehydrogenase genes, glyoxylate carboxylase genes and tartronic semialdehyde reductase genes. The method creatively discovers that the expression of the BASS6 gene in the plant is inhibited, the overexpression of the glycollate dehydrogenase gene, the glyoxylate carboxylase gene and the tartronic semialdehyde reductase gene is combined in chloroplast,the photorespiration can be significantly reduced, the photosynthetic efficiency of the plant can be improved, the biomass or the yield of the plant can be increased, more importantly, the drought tolerance of the transgenic plant is significantly higher than the drought tolerance of the plant inhibiting the PLGG1 gene expression, and the biomass or the yield under the drought condition is increased by 3-45% compared to the non-transgenic control.

Description

[0001] (1) Technical field [0002] The invention relates to a method for improving the photosynthetic efficiency of plants, which improves the photosynthetic efficiency, biomass or yield of plants through transgenic methods and improves plant planting resources. [0003] (2) Background technology [0004] The increase in the number of human beings and the improvement of living standards require more food and fodder to be consumed, which requires more food to be harvested on limited land. Therefore, it is very important to breed new high-yielding plant varieties. [0005] The photosynthetic products of the whole plant are all derived from the enzymatic catalysis of CO 2 into organic carbon compounds. Ribulose 1,5-bisphosphate carboxylase / oxygenase (RubisCO) is a carboxylase in the Calvin-Benson (CB) cycle. Since RubisCO with CO 2 or O 2 can react, RubisCO and O 2 The reaction produces phosphoglycolic acid, which enters the photorespiratory cycle, which leads to the waste ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/84C12N15/29C12N15/53C12N15/60A01H5/00A01H6/46A01H6/54
CPCC12N15/8218C12N15/8273C12N15/8269C12N9/0006C12N9/88C07K14/415C12Y101/99014C12Y401/01047C12Y101/0106
Inventor 张先文王东芳向雅琴沈志成
Owner ZHEJIANG UNIV
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