Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof

A technology of inhibitory concentration and detection method, applied in the field of microbial detection, can solve the problems of high price, insufficient reproducibility and quantification, cumbersome experimental process, etc. Accurate experimental results

Pending Publication Date: 2019-12-31
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The agar dilution method is the standard method of WHO, which has a long cultivation period and cumbersome experimental process
Disc diffusion method is expensive and lacks reproducibility and quantitation
The drug resistance gene detection probe has strong specificity, high sensitivity, good accuracy but expensive
The above method requires specialized technical personnel to operate relatively cumbersome and difficult to popularize

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof
  • Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof
  • Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A device for rapidly detecting the minimum inhibitory concentration of gonorrhoeae, including a test platform, such as image 3 As shown, the test platform includes a base layer 1, a test layer 2 and a feed layer 3, the test layer 2 is compounded between the base layer 1 and the feed layer 3, and the feed hole 4 is opened on the feed layer 3, and the test layer 2 corresponds to each feeding layer. The position of the hole 4 is provided with a test area, the test area is isolated from the blank position on the test layer by the first partition structure 5, and the second partition structure 6 is arranged between each test area and other adjacent test areas, such as figure 2 Shown; The first partition structure 5 and the second partition structure 6 are provided with partitions, such as image 3 shown.

[0044] The setting of the partition structure can isolate each part into a separate area without interfering with each other, so as to ensure the accuracy and reliabili...

Embodiment 2

[0056] The present invention also provides a preparation method of a device for rapidly detecting the minimum inhibitory concentration of gonorrhoeae, the preparation method is used to prepare the above-mentioned device, and includes the following steps:

[0057] (1) Use CorelDRAW to draw the shape and pattern of the design base layer, test layer and feeding layer, and print out the designed shape and pattern with a printer, cut, and cut off the first partition structure, the second partition structure and the third partition structure. The slots are filled with partitions;

[0058] (2) Wrap the test platform with tinfoil, place it on a heating plate, heat at 160°C for 3 minutes, put it under a UV lamp after the wax melts, and irradiate the front and back sides for 30 minutes each;

[0059] (3) Configure ceftriaxone standard solutions with different concentration gradients; the concentrations of ceftriaxone standard solutions are: 1 μg / mL, 0.5 μg / mL, 0.25 μg / mL, 0.125 μg / mL, 0...

Embodiment 3

[0066] The present invention also provides a detection method for rapidly detecting the minimum inhibitory concentration of gonorrhoeae, the detection method adopts the device prepared by the above preparation method, and includes the following steps:

[0067] 1) configure the Neisseria gonorrhoeae suspension;

[0068] ①Scrape an appropriate amount of Neisseria gonorrhoeae with the fungus collection ring, which was purchased from the American Type Culture Collection ATCC, and the preservation number is ATCC49226;

[0069] ② Put Neisseria gonorrhoeae in a 1.5mL centrifuge tube, add 1mL PBS buffer and mix well for testing.

[0070] ③Use a pipette gun to take 200ul of the Neisseria gonorrhoeae suspension and PBS buffer obtained in step ② into a 96-well plate, measure the optical density values ​​of the two with a spectrophotometer, and dilute the Neisseria gonorrhoeae suspension to a concentration of 10 -8 cfu / mL;

[0071] 2) Place the test platform in a petri dish filled with ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae. The device comprises a test platform, the test platform comprises a base layer, a test layer and a feed layer, the test layer is composited between that base layer and the feed layer, a feeding hole is arranged on the feed layer, a testing area is arranged on the testing layercorresponding to the position of each feeding hole, the testing area is isolated from the blank position on the testing layer through a first partition structure, a second partition structure is arranged between each testing area and other adjacent testing areas, and partitions are arranged in the first partition structure and the second partition structure. The invention also discloses a preparation method of the device, and also discloses a detection method for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae. The detection device of the invention has simple structural design and convenient carrying; the preparation method of the detection device is simple and the cost is low; the detection method of the invention can simply test the minimum inhibitoryconcentration of antibiotics, and the result is accurate.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, and in particular relates to a device, preparation and detection method for rapidly detecting the minimum inhibitory concentration of gonorrhoeae. Background technique [0002] Neisseria gonorrhoeae is a relatively common sexually transmitted pathogenic microorganism in clinical practice, which is highly infectious to humans. Neisseria gonorrhoeae mainly causes purulent infection of the genitourinary system, and can also cause combined or disseminated infections such as gonococcal ophthalmia, pharyngitis, proctitis, and pelvic inflammatory disease. An estimated 78 million new cases of N. gonorrhoeae occur each year in people aged 15-49 years. Up to now, gonorrhea caused by Neisseria gonorrhoeae has neither effective vaccine nor long-lasting immunity after being cured. As an infectious disease, it has been endangering human health. In the treatment of gonorrhea, the drug resistance o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/34C12M1/00C12Q1/18
CPCC12Q1/18
Inventor 王书崎王怡敏梁利国
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com