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Bifidobacterium animalis capable of promoting proliferation of bone marrow mesenchymal stem cells and application thereof

A technology of animal bifidobacteria and bone marrow mesenchyme, applied in the fields of biotechnology, food and medicine, to achieve good production performance and acid production performance

Active Publication Date: 2019-12-13
泰安大凡神农生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the inventors know, there is no report about probiotics, especially Bifidobacterium animalis, having a proliferative effect on bone marrow mesenchymal stem cells.

Method used

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  • Bifidobacterium animalis capable of promoting proliferation of bone marrow mesenchymal stem cells and application thereof
  • Bifidobacterium animalis capable of promoting proliferation of bone marrow mesenchymal stem cells and application thereof
  • Bifidobacterium animalis capable of promoting proliferation of bone marrow mesenchymal stem cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the isolation culture of lactic acid bacteria

[0039] 1 material

[0040] TPY medium; 2×Taq PCR MasterMix, DNA extraction kit (Tiangen Biochemical Technology Co., Ltd.);

[0041] 2. Method

[0042] 2.1 Isolation of Bifidobacteria

[0043] Add 5g of infant feces samples to 45mL of sterile saline and mix well, then gradiently dilute with sterile saline, select an appropriate concentration, and culture overnight on TPY medium in an anaerobic environment at 37°C by plate streaking method. The next day, typical colonies were taken for purification and culture, and a single colony was purified on TPY solid medium for 3-4 times until the microscopic bacteria were single.

[0044] 2.2 Identification of strains

[0045] Gram-stained smears of individual colonies were observed under the microscope. Inoculate TPY liquid medium for anaerobic culture at 37°C overnight, and use a DNA extraction kit to extract genomic DNA. The target fragment was amplified by PCR ...

Embodiment 2

[0069] Example 2: Isolation and culture of bone marrow mesenchymal stem cells

[0070] 1 material

[0071] High-sugar DMEM, 1% double antibody (penicillin / streptomycin): purchased from Hyclone Company; PBS buffer: NaCl 8‰, KCl 0.2‰, NaCl 2 HPO 4 12H 2 O 3.58‰, KH 2 PO 4 0.27‰; trypsin: containing 0.25% trypsin and 0.02% EDTA; fetal bovine serum: purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; Kunming mice: purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0072] 2 methods

[0073] The mice were killed by neck dislocation, the tibia and femur were taken on the ultra-clean table, the cancellous bone at both ends was cut open, the bone marrow mesenchymal stem cells were washed down with normal saline, centrifuged at 1500rpm for 10min, the supernatant was discarded, and the cells were washed with normal saline 2 After that, pipette and mix with 10% fetal bovine serum in high-sugar DMEM, spread on a 6-well plate and store in 37°C CO 2 Incubator...

Embodiment 3

[0076] Example 3: Proliferation of probiotics on MSCs

[0077] 1 material

[0078] cck8 was purchased from Beijing Suo Laibao Technology Co., Ltd.; trypsin + EDTA, high-glucose DMEM were purchased from hyclone company; 96-well plate and 6-well plate were purchased from star company.

[0079] 2 methods

[0080] 2.1 Subculture of cells

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PUM

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Abstract

The invention provides bifidobacterium animalis capable of promoting proliferation of bone marrow mesenchymal stem cells and an application thereof, which belong to the technical field of biotechnology and food and medicine. According to the invention, a strain of Bifidobacterium animalis NFTY9 with a good effect of promoting the proliferation of bone marrow mesenchymal stem cells is obtained by separation and screening for the first time, the strain has good production performance and acid production performance at the same time, and the characteristics show that the strain is a new probioticstrain with potential application prospects in the field of bone marrow mesenchymal stem cell research and treatment.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology, food and medicine, and specifically relates to an animal bifidobacterium strain capable of promoting the proliferation of bone marrow mesenchymal stem cells and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Probiotics are the general term for microorganisms that can have beneficial effects on the human body. At present, there are mainly Lactobacillus, Bifidobacterium, Saccharomyces, Clostridium butyricum, etc., which are more and more widely used in food, medicine, agriculture, animal husbandry, bioengineering, etc. Among them, bifidobacteria are one of the most impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N5/0775A23L33/135A23L29/00A61K35/745C12R1/01
CPCC12N1/20C12N5/0663A23L29/065A23L33/135A61K35/745A23V2002/00C12R2001/01C12N1/205A23V2400/515A23V2200/30
Inventor 单宝龙张建梅陈甜甜樊梅娜孙尹双白冬红刘红程秀芳党兰庆
Owner 泰安大凡神农生物有限公司
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