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A kind of culture medium and fermentation method for improving the content of functional red yeast open-loop lovastatin

A technology of functional red yeast rice and fermentation method, which is applied in the field of culture medium and fermentation to increase the content of functional red yeast rice open-loop lovastatin, can solve the problems of complex components, allergens, and high cost, and achieve a simple and efficient fermentation method. The effect of simple ingredients and high content

Active Publication Date: 2022-07-26
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing medium and fermentation methods of functional red yeast rice mostly use rice as a single substrate, and the components of the fermented product are simple and do not require post-treatment, but there is a problem of low lovastatin yield; while rice is compounded with other grains as a Fermented products with complex substrates may have a relatively high lovastatin content, but the open-loop lovastatin content is low, and its composition is complex, the cost is high, and there are safety hazards such as unpleasant taste and allergens. Hidden problems

Method used

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  • A kind of culture medium and fermentation method for improving the content of functional red yeast open-loop lovastatin
  • A kind of culture medium and fermentation method for improving the content of functional red yeast open-loop lovastatin
  • A kind of culture medium and fermentation method for improving the content of functional red yeast open-loop lovastatin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The medium components are: rice, soybean meal and L-glutamic acid, and the amount of soybean meal is 1.5% and the amount of L-glutamic acid is 2.5% based on 100 parts of the weight of rice.

[0028] 1) Preparation of strain activation solution

[0029] The Monascus purpureus CGMCC No.18110 strain was inoculated on PDA medium, and it was cultured at 32°C for 7 days for activation. Elute the spores on the medium with sterile water, shake them up, and make a concentration of 10 6 cfu / mL of spore suspension. The spore suspension was inoculated into the liquid seed medium at 5% of the inoculum amount, and cultured with shaking at 32° C. and 180 r / min for 48 hours to obtain the Monascus purpureus strain activation solution.

[0030] The components of liquid seed medium are: glucose 60g / L, peptone 25g / L, corn meal 10g / L, NaNO 3 2g / L, K 2 HPO 4 1g / L, MgSO 4 .7H 2 O1g / L, natural pH, sterilized at 121℃ for 20min.

[0031] 2) Preparation of fermentation medium

[0032] So...

Embodiment 2

[0039] The medium components are: rice, soybean meal and L-glutamic acid, and the amount of soybean meal is 2% and the amount of L-glutamic acid is 1.5% based on 100 parts of the weight of rice.

[0040] 1) Preparation of strain activation solution

[0041] The Monascus purpureus CGMCC No.18110 strain was inoculated on PDA medium, and it was cultured at 32°C for 7 days for activation. Elute the spores on the medium with sterile water, shake them up, and make a concentration of 10 6 cfu / mL of spore suspension. The spore suspension was inoculated into the liquid seed medium at 5% of the inoculum amount, and cultured with shaking at 32° C. and 180 r / min for 48 hours to obtain the Monascus purpureus strain activation solution.

[0042] The components of liquid seed medium are: glucose 60g / L, peptone 25g / L, corn meal 10g / L, NaNO 3 2g / L, K 2 HPO 4 1g / L, MgSO 4 .7H 2 O1g / L, natural pH, sterilized at 121℃ for 20min.

[0043] 2) Preparation of fermentation medium

[0044] Soak...

Embodiment 3

[0051] The medium components are: rice, soybean meal and L-glutamic acid, the amount of soybean meal is 1.75% and the amount of L-glutamic acid is 1.75% based on the weight of 100 parts of rice.

[0052] 1) Preparation of strain activation solution

[0053] The Monascus purpureus CGMCC No.18110 strain was inoculated on PDA medium, and it was cultured at 32°C for 7 days for activation. Elute the spores on the medium with sterile water, shake them up, and make a concentration of 10 6 cfu / mL of spore suspension. The spore suspension was inoculated into the liquid seed medium at 5% of the inoculum amount, and cultured with shaking at 32° C. and 180 r / min for 48 hours to obtain the Monascus purpureus strain activation solution.

[0054] The components of liquid seed medium are: glucose 60g / L, peptone 25g / L, corn meal 10g / L, NaNO 3 2g / L, K 2 HPO 4 1g / L, MgSO 4 .7H 2 O1g / L, natural pH, sterilized at 121℃ for 20min.

[0055] 2) Preparation of fermentation medium

[0056] Soak...

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Abstract

The invention discloses a medium and a fermentation method for increasing the content of functional red yeast open-loop lovastatin. The medium has simple components, low cost, and a simple fermentation method. Cyclovastatin is high. In the medium for improving the content of functional red yeast open-loop lovastatin of the present invention, the medium components are: rice, soybean meal and L-glutamic acid, and the amount of soybean meal based on 100 parts by weight of rice is 1.5 ~2%, and the dosage of L-glutamic acid is 1.5~2.5%.

Description

[0001] Field of Invention [0002] The invention relates to a culture medium and a fermentation method, in particular to a culture medium and a fermentation method for increasing the content of functional red yeast open-loop lovastatin. Background technique [0003] Red yeast rice is made from non-sticky rice and solid-state fermentation of red yeast rice. Mainly produced in Fujian, it is also called Fuqu and Danqu. Red yeast rice has been used in medicine and food for at least two thousand years in China. Monascus is mainly used in brewing, vinegar, fermented bean curd, food coloring and as traditional Chinese medicine in China. In 1979, Japanese scholar Endo Akira discovered and extracted the active substance lovastatin (Monacolin K), which inhibits cholesterol synthesis, from the culture solution of Monascus. is the main direction. [0004] In recent years, with the continuous improvement of living standards, coronary heart disease, hyperlipidemia, cerebral thrombosis, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12N1/14A23L7/104A23L33/135C12R1/645
CPCC12P1/02C12N1/14A23L7/104A23L33/135A23V2002/00C12R2001/645C12N1/145A23V2200/3262A23V2200/30
Inventor 洪厚胜于卓然陈程鹏
Owner NANJING TECH UNIV
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