Transmembrane anti-inflammatory peptide modified tumor-targeting multi-drug co-loaded liposome and preparation method thereof

Abstract

The invention belongs to the technical fields of biomedicine technology and nanomedicine, and discloses a tumor-targeted liposome drug delivery system with three drugs co-loaded. A multifunctional drug delivery system which actively targets tumors is prepared by the steps that cationic liposome is used as a basic carrier, anti-inflammatory drugs and anti-tumor drugs are loaded in the liposome at the same time, a transmembrane anti-inflammatory peptide is covalently linked to the surface of the liposome, and then the outer layer of the cationic liposome is coated with hyaluronic acid. The liposome simultaneously has the functions such as targeted accumulation and deep penetration of tumor tissue, specific recognition of tumor cells and efficient uptake of the tumor cells and interstitial cells thereof. The multifunctional liposome carrier can co-deliver the transmembrane anti-inflammatory peptide, the anti-inflammatory drugs and the anti-tumor drugs to the tumors, fully exerts the synergistic therapeutic effect of the combined drugs, blocks the key signaling pathways of excessive activation of the tumor cells and the interstitial cells, can reduce the level of inflammation and immunosuppression in the tumors, destroy tumor microenvironment, cuts off tumor cell migration pathways, and finally clears the tumor cells and tumor stem cells.

Description

technical field [0001] The invention belongs to the fields of biomedicine technology and nanomedicine technology, and relates to a membrane-penetrating anti-inflammatory peptide-modified multi-drug co-carrying liposome, a preparation method thereof and an application in anti-inflammatory synergistic anti-tumor targeted therapy. Background technique [0002] Studies have shown that chronic "uncontrollable inflammation" plays an important role in the occurrence and development of malignant tumors; chronic inflammation can promote the occurrence of different types of cancer, not only patients with specific inflammatory diseases are prone to cancer, but also tumors that have nothing to do with inflammation (such as Inflammatory microenvironment also exists in breast cancer). In the process of chronic inflammation-mediated tumorigenesis, inflammatory factors can weave tumor cells and their microenvironment into an inflammatory network, and NF-κB and STAT3 are the two key transcri...

AI Technical Summary

Problems solved by technology

At present, there have been many reports on multi-drug combined DDS, and some results have been achieved. However, these multi-drug combined DDS designed for tumor cells or for the tumor microenvironment have limitations, and most of them lack the ability to control tumor cells and tumor microenvironment. In the overall consideration of the overall complex system of the environment, the effect of blocking certain pathways or killing certain cells is local and temporary. For a powerful and meticulous tissue like a tumor, other pathways will be activated and activated to promote its more malignant development

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