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Total ginsenoside in stem-leaf content detection method of compound traditional Chinese medicine

A technology of ginseng stems and leaves and compound traditional Chinese medicine, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of increased testing costs, high production costs, waste of resources, etc., to reduce testing costs, increase service life, and improve accuracy Effect

Active Publication Date: 2019-12-06
INNER MONGOLIA CONBA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Shexiang Tongxin Dropping Pills use precious medicinal materials, and its production cost is relatively high. The Pharmacopoeia method has already used 40 pills. Continuing to increase the amount of medicine for enrichment and extraction will increase the cost of testing and waste resources.
Therefore, there is still a lack of an accurate, rapid and low-cost method for detecting the total saponins of ginseng stems and leaves in Musk Tongxin Dripping Pills.

Method used

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  • Total ginsenoside in stem-leaf content detection method of compound traditional Chinese medicine
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  • Total ginsenoside in stem-leaf content detection method of compound traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the accelerated test of Musk Tongxin Dropping Pills

[0049] Store the Musk Tongxin Dropping Pills in a temperature: 40°C ± 2°C, relative humidity: 75% ± 5% in a water-proof electric heating constant temperature incubator for accelerated testing, and store for the 0th month, the first month, and the second month , 3 months, and 6 months were sampled respectively, and the dripping pills were dried at the end of 6 as a control group, and the total saponin content of ginseng stems and leaves was determined according to the Pharmacopoeia method. The results are shown in Table 4 below:

[0050] Table 4 Musk Tongxin Dropping Pills Accelerated Test

[0051]

[0052] Note: a. The content of 0.060 mg / pill of ginseng stem and leaf total saponins required by the Pharmacopoeia is 100%. More than 100% means that the content is higher than the requirements of the Pharmacopoeia, and less than 100% means that the content is lower than the requirements of the Pharmacop...

Embodiment 2

[0054] Example 2: Investigation on the Impurity Removal Method of Ginseng Stem and Leaf Total Saponins

[0055] 1. Method category

[0056] 1.1 Direct extraction

[0057] Preparation of the test solution Take about 1.0 g of Musk Tongxin Dropping Pill powder in a 100 ml conical flask with a stopper, add 20 ml of pure water, and ultrasonicate for 1 h. Filter through a 0.45 μm microporous membrane, and take the subsequent filtrate to obtain the obtained product.

[0058] 1.2 Column purification

[0059] (1) Take about 1.0 g of Shexiang Tongxin Dropping Pills powder in a 150ml Erlenmeyer flask with a stopper, 2 parts, accurately weighed, add 50ml of pure water, and ultrasound for 1h. Centrifuge at 10,000rpm for 10min, pipette 25ml of the supernatant and load it on D-101 macroporous adsorption resin, elute with 3BV methanol, collect the eluate and evaporate to dryness in a water bath, dissolve the residue with methanol and dilute it to a 5ml volumetric flask . Filter through a...

Embodiment 3

[0079] Example 3: Optimizing the pretreatment process of total saponins from ginseng stems and leaves

[0080] 1. Investigate the process content

[0081] 1.1. Investigation of extraction solvent

[0082] (1) Take 40 pills of Shexiang Tongxin Dropping Pills, put them in a stoppered Erlenmeyer flask, accurately pipette 50ml of methanol, ultrasonicate for 10min, fully dissolve, centrifuge at 10000rpm for 10min, take the supernatant and evaporate to dryness, and reconstitute the residue with 40ml of water. Wash twice with ethyl acetate, 40ml each time, separate the water layer and dilute to 50ml with water, accurately pipette 10ml and evaporate to dryness, dissolve the residue with water and dilute to 5ml volumetric flask. Centrifuge at 13000rpm for 10min, and take the filtrate to obtain the final product.

[0083] (2) Take 40 pills of Shexiang Tongxin Dropping Pills, put them in a stoppered Erlenmeyer flask, accurately pipette 50ml of pure water, ultrasonicate for 10min, fully...

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Abstract

The invention provides a total ginsenoside in stem-leaf content detection method of compound traditional Chinese medicine. The detection method comprises the following steps: (1) preparation of a sample for test: ultrasonically centrifuging to obtain supernatant after adding water to dissolve, washing by using ethyl acetate, separating a water-taking layer and drying by distillation, and re-dissolving by using water to metered volume; (2) preparation of a reference substance; (3) gradient elution; and (4) determination. Through the method provided by the invention, the problem that the total ginsenoside in stem-leaf detected content in the pharmacopeia method is lower than the actual content is solved, and the operation is safe and environment-friendly; and the test period is effectively shortened, and the cost is lowered.

Description

technical field [0001] The invention relates to a method for detecting the content of effective components of a compound traditional Chinese medicine, in particular to a method for detecting the content of total saponins in stems and leaves of ginseng. Background technique [0002] Shexiang Tongxin Dropping Pills are made according to the ancient prescriptions "Zhibaodan" and "Liushen Pills". The king medicine of the prescription is artificial musk and toad venom, which are fragrant and clears the veins, relieves spasms and relieves pain; the ministerial medicine, total saponins of ginseng stems and leaves, salvia miltiorrhiza, nourishes the heart, activates blood and removes blood stasis; the adjuvant medicine, artificial bezoar and bear bile powder, clears the pulse, cools the pulse, resolves phlegm and expels blood stasis; Make the medicine borneol resuscitate and refresh the mind, and directly reach the lesion. The combination of various medicines is aromatic, invigorat...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/14G01N30/54G01N30/74G01N2030/027G01N2030/062G01N2030/146
Inventor 周祥吴华铃王如伟金辉辉杨千千叶剑锋宋永标麻懿文刘玮峰李学臻
Owner INNER MONGOLIA CONBA PHARMA CO LTD
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