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Method of promoting colonization of delftia tsuruhatensis at plant rhizospheres

A technology of Crane Haneda Delft and plant roots, which is applied in the field of promoting the colonization of Crane Haneda Delftia in the plant rhizosphere, can solve the problems that have not been solved, and achieve the goal of increased gene abundance, good application value, and enhanced growth-promoting effect Effect

Active Publication Date: 2019-11-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no research report on the use of mucic acid as a signaling molecule to induce the colonization of Delftia chinensis in the plant rhizosphere

Method used

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  • Method of promoting colonization of delftia tsuruhatensis at plant rhizospheres
  • Method of promoting colonization of delftia tsuruhatensis at plant rhizospheres
  • Method of promoting colonization of delftia tsuruhatensis at plant rhizospheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Chemotaxis of organic acids in different root exudates to Delftia 10492

[0039]The present invention selects 10 kinds of organic acids (L-quinic acid, D-fructose, D-malic acid, L-alanine, D-galacturonic acid, L-lactic acid, propionic acid, mucic acid, 3-hydroxybutyric acid and L-glutamic acid), to study its chemotaxis to Delftia crascens, the specific experimental methods and experimental results are as follows:

[0040] 1. Experimental method

[0041] Use a pipette to draw 100 μL of Delftia 10492 bacteria liquid, use a syringe to draw 200 μL of an organic acid solution with a concentration of 30 μm / L, and set sterile water as a blank control. After inserting the syringe into the tip of the pipette, let it stand in the ultra-clean bench for 2 hours, take out the organic acid solution in the syringe and dilute it with sterile water for 10, 10 2 、10 3 、10 4 、10 5 、10 6 times, spread to LB medium, set up 3 parallels, place them in a biochemical incubator, ...

Embodiment 2

[0044] Example 2 The group swimming effect of organic acids in different root exudates on Delftia 10492

[0045] The present invention selects 12 kinds of organic acids (L-malic acid, acetic acid, L-glutamic acid, L-serine, D-gluconic acid, propionic acid, D-quinic acid, L-arginine, tartaric acid, α-ketone glutaric acid, L-pyroglutamic acid and mucic acid), to study its group swimming effect on Delftia Crane Haneda, the specific experimental methods and experimental results are as follows:

[0046] 1. Experimental method

[0047] Prepare LB solid medium containing 30 μm / L organic acid (the blank control is sterile water), place an 8 mm sterile filter paper in the center of the solid culture, and suck 5 μL of the bacterial suspension of Delftia tsuruhanata 10492 on the filter paper central. Set up 3 parallels, place them in a biochemical incubator and culture them at 28°C for 4 days, measure the diameter from 3 different directions, calculate the average value of 3 parallels,...

Embodiment 3

[0050] Example 3 Effect of Mucic Acid on the Colonization of Delftia Tsuruhanata 10492 in the Roots of Whole Red Amaranth

[0051] According to the results of Examples 1 and 2, mucic acid, which has the strongest chemotaxis and group-swimming effect on Delftia tsuruhanata 10492, and malic acid, which is second to the chemotaxis and group-swimming effect, were subjected to in vitro induction experiments, specifically The experimental method and experimental results are as follows:

[0052] 1. Experimental method

[0053] Prepare whole Hoagland nutrient solution with a concentration of 1 / 4, add 50mL Hoagland nutrient solution and 1% agar to each tissue culture bottle to make a solid medium. The whole red amaranth seeds were soaked in 2% sodium hypochlorite solution for 30 minutes, placed in the center of the tissue culture bottle, and cultivated for 2 days. After the tender leaves grow, suck 10 μL of the bacterial solution of Delftia tsuruhanata 10492 at a place 2 mm away from...

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Abstract

The invention discloses a method of promoting colonization of delftia tsuruhatensis at plant rhizospheres, and provides application of mucic acid in the aspects of promoting colonization of delftia tsuruhatensis at the plant rhizospheres and preparing preparations for promoting colonization of delftia tsuruhatensis at the plant rhizospheres. By adopting the method, a signal molecule, namely mucicacid capable of promoting colonization of delftia tsuruhatensis at the plant rhizospheres is screened out from secretions of plant root systems, research shows that mucic acid has significant chemotactic and swarming effects on delftia tsuruhatensis and can significantly promote colonization of delftia tsuruhatensis at the plant rhizospheres; besides, when mucic acid and delftia tsuruhatensis comeinto play together, reproduction of delftia tsuruhatensis at the plant rhizospheres can be significantly promoted; the total fresh weight, the total root weight, the plant height, the chlorophyll content and the root system nifH gene abundance of plants are significantly increased; a growth-promoting effect on the plants is further improved significantly, and thus the method has excellent application value and broad development space.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to a method for promoting the colonization of Delftia tsuruhanata in the rhizosphere of plants. Background technique [0002] Plant diseases are the great enemy of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the average annual loss of production due to plant diseases is 10% to 15% of the total output. In addition to the loss of plant diseases leading to reduced yields and lower crop quality, the pesticides used to prevent and control diseases will also increase agricultural costs and cause environmental pollution and public nuisance. Therefore, the scientific control of plant diseases is always an important task in agricultural production. In recent years, people have paid more and more attention to the use of plant rhizosphere growth-promoting bacteria (PGPR), because the functional strains...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06A01G13/00C12R1/01
CPCC12N1/20A01G7/06A01G13/00C12R2001/01C12N1/205
Inventor 李取生杨俊卿王燚凡臧朦朦
Owner JINAN UNIVERSITY
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