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Method for detecting lipid in skin stratum corneum and application thereof

A detection method and technology for stratum corneum, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of high requirements for operating technicians, high cost of mass spectrometers, and high use costs, and achieve simple extraction process and low cost of instrument consumables. , to meet the effect of effective detection

Inactive Publication Date: 2019-11-15
ZHUHAI EASYCARE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry) technology is mainly used. This technology can directly identify lipid compounds (components in lipids) so as to avoid the influence of ultraviolet absorption of mobile phase. However, it is very troublesome to go through a series of separation and purification operations in the quantitative analysis of organic matter. Moreover, mass spectrometers are expensive, costly to use, and have high requirements for operating technicians. Although they are suitable for scientific research institutions

Method used

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  • Method for detecting lipid in skin stratum corneum and application thereof
  • Method for detecting lipid in skin stratum corneum and application thereof
  • Method for detecting lipid in skin stratum corneum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A method for detecting lipids in the stratum corneum of the skin, comprising the following steps:

[0059] (1) Sampling: Take the tape and stick it on the face skin of the subject, then take off the tape and immerse it in methanol, then process it with an ultrasonic breaker, take the supernatant, and then filter it through a filter membrane to obtain the filtrate, which is set aside;

[0060] (2) Configuration of standard solution: configure standard solution of ceramide, standard solution of cholesterol, standard solution of linoleic acid, standard solution of squalene, standard solution of oleic acid, for standby;

[0061] (3) Build a standard curve: the standard solution prepared by step (2) is measured by HPLC method respectively, and the standard curves of ceramide, cholesterol, linoleic acid, squalene and oleic acid are obtained respectively, and the standard curve is established The functional relationship between the peak area of ​​ceramide, cholesterol, linolei...

Embodiment 2

[0082] A method for detecting lipids in the stratum corneum of the skin, comprising the following steps:

[0083] (1) Sampling: Take the tape and stick it on the subject's facial skin, then take off the tape and immerse it in a mixture of methanol and isopropanol. The volume ratio of methanol to isopropanol is 70:30, and then use an ultrasonic breaker to processing, taking the supernatant, and then filtering through a filter membrane to obtain a filtrate for subsequent use;

[0084] (2) Configuration of standard solution: configure standard solution of ceramide, standard solution of cholesterol, standard solution of linoleic acid, standard solution of squalene, standard solution of oleic acid, for standby;

[0085] (3) Build a standard curve: the standard solution prepared by step (2) is measured by HPLC method respectively, and the standard curves of ceramide, cholesterol, linoleic acid, squalene and oleic acid are obtained respectively, and the standard curve is established T...

Embodiment 3

[0119] According to the method described in Example 2, 227 subjects with oily, normal and dry skin types, aged 18-50 years, regardless of gender, were sampled from the right cheek and forehead of the subjects , finally calculated squalene, fatty acid (W fatty acid=(W linoleic acid / 0.25) / 2 in the experimenter's lipid of three skin types through HPLC detection, in addition, the content of described fatty acid also can be obtained by oil The content of acid is estimated to obtain), the average value of cholesterol, and the results are shown in Table 1.

[0120] Table 1:

[0121]

[0122] The impact of the average squalene and average fatty acid content in Table 1 on spots or acne is as follows: For the average squalene, 0.65 and below means, 0.65-0.9 means there is spot potential, 1.0-1.3 Indicates spots, 1.4-2.0 indicates acne potential, 2.0 or more indicates acne; in terms of fatty acid average, 0.076 and below indicate severe spots, 0.12-0.24 indicate potential spots, 0.24-0...

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Abstract

The invention, which belongs to the field of substance detection, discloses a method for detecting lipid in the skin stratum corneum. The method comprises: carrying out sampling; carrying out configuration of a standard solution; building a standard curve; calculating the concentration of a lipid component in the filtrate; and calculating the content of lipid in skin stratum corneum of a subject.Compared with the prior art, the disclosed method has the following advantages: the skin sampling is non-traumatic sampling and no toxic chloroform is used, so that the safety is improved; the detecting method can detect the ceramide, cholesterol, squalene, linoleic acid and oleic acid and other components efficiently and simultaneously, so that the consumable, reagent and time costs are saved; onthe basis of the detection results, the proportion of lipid components in the skin of the subject is deduced to guide the amount of lipid components in cosmetics, so that the skin's absorption of thebeneficial components in cosmetics is promoted and thus the good makeup effect is realized.

Description

technical field [0001] The invention belongs to the field of substance detection, and in particular relates to a method for detecting lipids in the stratum corneum of the skin and an application thereof. Background technique [0002] The skin is the largest organ of the human body, covering the human body surface, serving as the interface between the internal environment and the first line of defense against external stimuli. The appearance of the skin reflects the health and age of the body. [0003] The stratum corneum is located in the outermost layer of the epidermis. It is composed of about 50 layers of dead flat keratinocytes. It is responsible for the skin barrier function. It is the initial part of the action of cosmetics and the main speed-limiting part of cosmetic penetration. Therefore, improving the permeability of the stratum corneum to cosmetics can better promote the skin's absorption of beneficial components in cosmetics and maintain skin vitality and health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 粟雨桑何海鸥周立武王德友
Owner ZHUHAI EASYCARE TECH CO LTD
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