Lactobacillus fermentum strain and its application

A technology of Lactobacillus fermentum and strains, applied in Lactobacillus, application, bacteria and other directions, can solve the problem of less Lactobacillus fermentum products, achieve the ability to repair colonic mucosal damage, down-regulate the level of pro-inflammatory cytokines, and achieve excellent results. Effect

Active Publication Date: 2021-10-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the application of Lactobacillus fermentum and its products has been involved in many fields such as medical care, food industry, and animal husbandry, there are relatively few Lactobacillus fermentum products that are really high-quality and meet market demand.

Method used

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  • Lactobacillus fermentum strain and its application
  • Lactobacillus fermentum strain and its application
  • Lactobacillus fermentum strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 strain screening and identification

[0029] 1. Strain screening and identification

[0030] The stool samples of healthy elderly aged between 100 and 128 years old who had not taken any antibiotics or Lactobacillus preparations within 2 months in Bama County, Guangxi, and lived in scattered locations were collected and stored frozen. After thawing, the stool samples were dissolved with sterile phosphate buffer solution and diluted to gradient concentrations, inoculated on the modified MRS solid medium with 2% inoculum, and cultured at 37°C. The "improved MRS solid medium" refers to the MRS solid medium containing calcium carbonate (CaCO 3 ) and 5-bromo-4-chloro-3-indole-β-D-galactoside (X-gal), CaCO 3 The mass percent concentration of X-gal is 0.8%, and the concentration of X-gal is 200 μl of 2% X-gal per 200 mL of culture medium.

[0031] Pick a single colony with a calcium-dissolving circle or a light blue color and streak for multiple generations of pur...

Embodiment 2

[0050] The characteristic sequence of embodiment 2 LTP1332

[0051] The LTP1332 genome consists of 1 chromosome and 2 plasmids. The chromosomal genome is circular. The average content of the sum of guanine (G) and thymine (C) is 51.3%. The length is 2,149,768bp and contains 2,218 independent coding regions (CDSs), the average length is 835bp, and the total length of the coding region accounts for 86.18% of the whole genome. The whole gene of LTP1332 is shown in the picture Figure 4 .

[0052] Comparing the LTP1332 nucleotide sequence with the NCBI database, there are 16 nucleotide sequences in the LTP1332 genomic DNA, which reflect the unique characteristics of the LTP1332 genome. These 16 characteristic sequences are shown as SEQ ID NO: 1 to SEQ ID NO: 16 shown.

Embodiment 3

[0053] Example 3 LTP1332 growth curve and passage cycle

[0054] Inoculate the LTP1332 seed liquid in the MRS liquid medium with 1% inoculation amount, measure the number of viable bacteria and reducing sugar content in the fermentation broth every 3h, take the fermentation time (h) as the abscissa, and take the logarithmic value of the number of viable bacteria (Log CFU / mL) and reducing sugar content (g / L) as the ordinate, drawn as Figure 5 The growth curve of Lactobacillus fermentum LTP1332 is shown. Depend on Figure 5 It was concluded that LTP1332 entered the logarithmic phase of growth after 6 hours of cultivation, entered the stable phase at about 24 hours, and the bacterial concentration reached the maximum, 24 to 39 hours, the bacterial concentration did not change significantly, and the bacterial concentration began to decline at 39 hours, entering the decline period.

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Abstract

The present invention relates to Lactobacillus fermentum strain LTP1332 and application thereof. The preservation number of the strain is CCTCCM 2019029, which can exist in a living form or in a non-living form. The living form is preferably in the form of freeze-dried powder, which can be used in food, health care products, medicines, food supplements, and pharmaceuticals. Application, preferably for anti-inflammatory antibacterial and probiotics. LTP1332 has a strong tolerance to the gastrointestinal environment, has a strong gastrointestinal tolerance, can pass through the gastrointestinal tract of the body smoothly, exerts a probiotic function, and has a good ability to repair colonic mucosal damage. DSS-induced mice exhibit anti-inflammatory effects and down-regulate the levels of pro-inflammatory cytokines, and the effect is better than that of the model strain CCIC22537.

Description

technical field [0001] The invention belongs to the field of biotechnology; in particular, it relates to strains of Lactobacillus fermentum and applications thereof. Background technique [0002] Lactobacillus fermentum (Lactobacillus fermentum) belongs to the Lactobacillus genus in the Lactobacillus family. It is a normal flora in the intestinal tract, oral cavity and vagina of humans and animals. It has the ability to maintain the stability of the intestinal microflora and improve the internal environment of the host body. One of the most important species of Lactobacillus for human health. [0003] The main ways for Lactobacillus fermentum to exert antibacterial effect are: the organic acid produced by fermentation reduces the pH value of the microenvironment of the body, inhibits acid-resistant pathogenic bacteria and spoilage bacteria; competes with pathogenic bacteria for gastrointestinal binding sites and nutrients, Prevents adherent colonization of pathogenic bacter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P29/00A61K8/99A61Q17/00A23K10/16A23K10/18A23L33/135C12R1/225
CPCA61K35/747A61P31/04A61P29/00A61K8/99A61Q17/005A23K10/16A23K10/18A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/143A23V2200/30A23V2200/3204
Inventor 李全阳吴治邦郝丹
Owner GUANGXI UNIV
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