Method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography

Abstract

The invention discloses a method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography. According to the method, two bitterants in a sample of the finger painting inkpad of children are extracted in an ultrasonic extracting mode by taking methyl alcohol-0.02 mol / L KH2PO4 (30:70, v / v) as a solvent; and the two bitterants are quantified through an external standard method and are analyzed through the UPLC. The linear ranges of the naringin and the denatonium benzoate are both 0.1 to 5 mg / L (R2is greater than or equal to 0.99), and the detection limits (S / N>3) are respectively 0.75 and 1.15 mg / kg. The mark recovery rate ranges of the naringin and the denatonium benzoate in the finger painting inkpad for the children at three concentration levels are 86.7 to 108.4 percent; and the relative standard deviation (RSD, n=6) is less than 5.0 percent.

Description

Technical field [0001] The invention relates to a detection method, in particular to a method for determining the bitter agents naringin and denatonium benzoate in children's finger painting ink pad by ultra-high performance liquid chromatography. Background technique [0002] Aversive agent (Aversive agent), also known as aversive agent, anti-feeding agent, is added to products to make the taste or smell bitter to prevent people, especially children, from inhaling or ingesting toxic and harmful substances, rodents and birds Chemical agents widely used to destroy facilities. Denatonium benzoate, also known as Bitrex, whose structure is composed of benzoic acid groups and denatonium groups, is the most bitter compound currently known and a typical representative of bittering agents. Bitter essence is chemically stable, inexpensive, easy to obtain, and easily soluble in water. In addition, its Median lethal dose (LD50) of mammals is 841 mg / kg body weight, and only a very small amo...

AI Technical Summary

Problems solved by technology

[0005] The matrix of children’s finger painting ink pads is complex. In order to avoid interference, it is necessary to add an extraction solvent with a high dilution factor for extraction. The standard recommended concentration of denatonium benzoate is 4 mg / kg, which is a challenge for the sensitivity of the instrument and method. The sensitivity of the instrument and assay method of above-mentioned denatonium benzoate can not meet the requirement

[0006] Children's finger painting inkpad is one of the finger painting pigments. Children use their fingers to dip the paint on the inkpad for creation. Children's consciousness is weak. If children's inkpad products do not add bittering agents or the amount of addition does not meet the standard requirements, it is easy to lick children's body and mind. serious health hazard

Ultra-high performance liquid chromatography has the advantages of fast analysis speed and high separation efficiency. At present, there is no report on the analysis of bittering agents naringin and denatonium benzoate in children's finger painting ink pads by ultra-high performance liquid chromatography.

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