Method for detecting adenosine triphosphate by paper-based dual-mode electrochemical sensor

A nucleoside triphosphate and detection zone technology is applied in the detection field of adenosine nucleoside triphosphate, which can solve the problems of insufficient sensitivity, limited application and complexity, and achieves the effects of improving sensitivity, reducing experimental cost and light weight.

Inactive Publication Date: 2019-11-12
UNIV OF JINAN
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Problems solved by technology

[0003] In recent years, many methods have been developed to detect ATP, such as photoelectrochemical aptasensor, surface-enhanced Raman scattering, photomagnetic aptasensor, electrochemiluminescence, fluorescence spectroscopy, and chemiluminescence resonance energy transfer, These methods show clear advantages for adenosine triphosphate detection, however, usually involve complicated manipulations, insufficient sensitivity, expensive equipment and large background interferences, which limit their application

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  • Method for detecting adenosine triphosphate by paper-based dual-mode electrochemical sensor
  • Method for detecting adenosine triphosphate by paper-based dual-mode electrochemical sensor
  • Method for detecting adenosine triphosphate by paper-based dual-mode electrochemical sensor

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Embodiment 1

[0023] Example 1 (from human serum)

[0024] A method for detecting adenosine triphosphate with a paper-based dual-mode electrochemical sensor with simple operation, fast detection speed and low cost, comprising the following steps:

[0025] 1. Use Adobe Illustrator CS6 software on the computer to design a cross-shaped hydrophobic wax printing pattern and use a wax jet printer to print it in batches on cut A4 size filter paper, and then heat it on a heating plate until the wax melts and penetrates the entire paper Thickness, forming a hydrophobic area, the style is as attached figure 1 As shown, 1 is the electrode label, 2 is the detection area, 3 is the hollow area, 4 is the reaction area, and 5 is the supercapacitor label.

[0026] 2. Using screen printing method, print the carbon working electrode to the detection area, print the Ag / AgCl reference electrode and carbon counter electrode to the electrode label, print the carbon electrode to the reaction area, and print the c...

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Abstract

The invention relates to a method for detecting adenosine triphosphate by a paper-based dual-mode electrochemical sensor and discloses a method for detecting adenosine triphosphate in a paper-based dual-mode manner. A hydrophobic region and a hydrophilic region are fabricated on paper by a wax printing technology, an electrode and a wire are printed by a silk-screen printing technology, and folding equipment is formed by a cutting technology. Different regions of a paper chip are functionalized by various types of methods, an instantaneous current is detected by a universal meter by specific identification effects of the adenosine triphosphate and an aptamer, specificity catalysis of glucose oxidase on glucose and an energy storage effect of a supercapacitor, so that the sensitivity is improved, and ultra-sensitive electrochemical detection of a to-be-detected object can be achieved by an electrochemical working station.

Description

technical field [0001] The invention relates to a method for detecting adenosine triphosphate by a paper-based dual-mode electrochemical sensor, and belongs to the technical field of detection of adenine triphosphate. Background technique [0002] Adenosine triphosphate, as the main fuel molecule in almost all forms of life, plays an important role in the regulation of biochemical reactions such as gene replication, cellular metabolism, biosynthesis, drug delivery, immunity and neural mediation. It has been found that the abnormal concentration and ratio of adenosine triphosphate in organisms is closely related to many diseases, including hypoxia, hypoglycemia, ischemia, Parkinson's disease and some malignant tumors. Therefore, accurate and rapid detection of ATP before reaching life-threatening levels is very important in biochemical research and clinical diagnosis. [0003] In recent years, many methods have been developed to detect ATP, such as photoelectrochemical aptas...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/308
Inventor 于京华刘悦崔康张丽娜周晨曦
Owner UNIV OF JINAN
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