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Method for rapidly predicting fruiting capacity of lentinula edodes strains

A technology for mushrooms and strains, applied in the field of fungal enzymes, can solve problems such as unreported fruiting ability, and achieve the effects of reducing economic loss and time loss, saving time and reducing economic cost.

Active Publication Date: 2019-11-08
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

However, there is no report on the experimental research on the relationship between the change of enzyme activity in the early hyphal stage of the strain and the fruiting ability.

Method used

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  • Method for rapidly predicting fruiting capacity of lentinula edodes strains
  • Method for rapidly predicting fruiting capacity of lentinula edodes strains

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Embodiment 1

[0030] A method for rapidly predicting fruiting ability of mushroom strains, the specific steps are as follows:

[0031] (1) Pre-cultivation of shiitake mushroom mycelia. Inoculate 36 kinds of common shiitake mushroom hyphae on PDA plates for activation and culture for 10 days, punch holes at the edge of the mycelia of the activated strains (perforation aperture d=5mm), and take another PDA plate with a medium thickness of 3-4 mm on the PDA plate. And inoculated in the center of the plate, repeated the above operation 5 times, cultured the inoculated PDA plate in the incubator, the culture temperature was 23°C, and the culture time was 13 days, until obvious new hyphae could be seen around the inoculated block and the edges of the colonies were clear. Use the cross method to draw a line every 24 hours, measure the diameter of the bacterial strain, record the growth rate of the bacterial strain, repeat the above operation, and stop drawing the line when the diameter of the bact...

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Abstract

The invention relates to a method for rapidly predicting fruiting capacity of lentinula edodes strains. Low melting-point agarose containing guaiacol is used as a chromogenic substrate, lentinula edodes hypha obtained by preculture is inoculated to the chromogenic substrate, after an enzymatic reaction, an OD value of the chromogenic substrate is read by a spectrophotometric method, concentrationof the low melting-point agarose and the guaiacol in the chromogenic substrate are separately 1% and 0.02%, and a melting point of the low melting-point agarose is 65 DEG C. According to the method for rapidly predicting the fruiting capacity of the lentinula edodes strains, a chromogenic qualitative method is combined with the spectrophotometric method, continuous change of enzyme activity of themass strains can be rapidly detected, the fruiting condition can be predicted according to an obtained enzyme activity result, the maximum time is no more than 13 days from hypha preculture to enzymeactivity detection, and the prediction accuracy reaches up to 85% or above.

Description

technical field [0001] The invention belongs to the technical field of fungal enzymes, and in particular relates to a method for rapidly predicting the fruiting ability of mushroom strains. Background technique [0002] At present, there are many methods for measuring fungal enzyme activity, and there is no unified standard. These methods can be divided into two categories, qualitative analysis methods and quantitative analysis methods, which are based on the analysis of compounds produced by extracellular enzymes and substrates. For example, the substrates of laccase are generally guaiacol, ABTS, ortho-toluene, etc.; the three hydrolases of cellulase generally use filter paper as substrates; , also need to add catechol as a substrate. The enzyme qualitative analysis method is generally the flat color circle method, and the enzyme activity is simply judged according to the appearance of the color circle and the color depth. This type of method is simple and quick to operat...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCC12Q1/02
Inventor 尚晓冬章炉军张美彦谭琦宋春艳李良敏李玉周峰
Owner SHANGHAI ACAD OF AGRI SCI
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