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A method for enhancing denitrification and denitrification in horizontal subsurface flow wetlands

A technology of denitrification and denitrification and horizontal subsurface flow, which is applied in the field of denitrification and denitrification enhancement in horizontal subsurface flow wetlands, and can solve the problem that the number of aerobic denitrification bacteria is small, the advantages are not obvious, and the denitrification and denitrification process of horizontal subsurface flow wetlands cannot be effectively completed. To achieve the effect of realizing aerobic denitrification operation, wide application prospects, ensuring biological activity and denitrification performance

Active Publication Date: 2021-06-15
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, researchers have gradually discovered the existence of aerobic denitrifying bacteria, but people have isolated some aerobic denitrifying bacteria in various environments such as soil, ditches, ponds, activated sludge, sediments, etc. However, due to the small number of aerobic denitrifying bacteria, the advantages are not obvious, and the denitrification and denitrification process in horizontal subsurface flow wetlands cannot be effectively completed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Step 1: strain acclimatization, first prepare carbon source, nitrogen source, activated sludge after enrichment culture and culture solution, concentrate the activated sludge, obtain sludge concentrate, and set aside; then put the culture solution prepared in step A into In a container with a capacity of 15L, add sludge concentrate, adjust the pH to 6, and then add carbon source and nitrogen source, so that the carbon content in the container is 390ppm, the nitrogen content is about 19.5ppm, and the carbon-nitrogen ratio is C / N=20; Finally, the small air pump is aerated so that the dissolved oxygen in the container is DO≥5, cultured at room temperature, and the carbon source and nitrogen source are added once every 24 hours, and the dosage of carbon source and nitrogen source The dosage is the same as that in step B. In one cycle, the aeration time of the air pump is 23.5h, and the time for settling and putting in carbon source and nitrogen source is 0.5h; the domestica...

Embodiment 2

[0062] Step 1: strain acclimatization, first prepare carbon source, nitrogen source, activated sludge after enrichment culture and culture solution, concentrate the activated sludge, obtain sludge concentrate, and set aside; then put the culture solution prepared in step A into Add sludge concentrate to a container with a capacity of 15L, adjust the pH to 7, and then add carbon source and nitrogen source, so that the carbon content in the container is 400ppm, the nitrogen content is about 20ppm, and the carbon-nitrogen ratio is C / N = 20; Finally, a small air pump was used to aerate, so that the dissolved oxygen in the container was DO ≥ 5, and the culture was carried out at room temperature, and the carbon source and nitrogen source were added once every 24 hours. In step B, the dosage is the same. In one cycle, the aeration time of the air pump is 23.5h, and the time for sedimentation and carbon source and nitrogen source is 0.5h; the continuous culture is 6 days, and the dome...

Embodiment 3

[0066] Step 1: strain acclimatization, first prepare carbon source, nitrogen source, activated sludge after enrichment culture and culture solution, concentrate the activated sludge, obtain sludge concentrate, and set aside; then put the culture solution prepared in step A into In a container with a capacity of 15L, add sludge concentrate, adjust the pH to 7, and then add carbon source and nitrogen source, so that the carbon content in the container is 410ppm, the nitrogen content is about 20.5ppm, and the carbon-nitrogen ratio is C / N=20; Finally, the small air pump is aerated so that the dissolved oxygen in the container is DO≥5, cultured at room temperature, and the carbon source and nitrogen source are added once every 24 hours, and the dosage of carbon source and nitrogen source The dosage is the same as that in step B. In one cycle, the aeration time of the air pump is 23.5h, and the time for sedimentation and carbon source and nitrogen source is 0.5h; the continuous cult...

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Abstract

The invention discloses a method for enhancing denitrification and denitrification in horizontal subsurface flow wetlands. The invention includes three processing steps of strain domestication, strain screening, and strain release. Firstly, the enrichment of aerobic denitrifying bacteria is achieved through domestication and cultivation. And improve the denitrification advantages of the strains to obtain domesticated strains; at the same time, after obtaining the domesticated strains, in this technical plan, through multiple screenings of the domesticated strains, the aging strains in the sludge and some bacteria that cannot be denitrified can be removed. The bacterial strains that have a good denitrification effect can be obtained as the strains that have a good denitrification effect; finally, the strains that have been screened are combined with the biological carrier to obtain immobilized fillers; the loss of strains is prevented; the process of the present invention The operation is simple, which not only effectively realizes the aerobic denitrification operation of the horizontal subsurface flow wetland, but also ensures the biological activity and denitrification performance of the aerobic denitrification bacteria, and enhances the denitrification of the horizontal subsurface flow wetland.

Description

technical field [0001] The invention relates to the technical field of wetland treatment, in particular to a method for enhancing denitrification and denitrification in horizontal subsurface flow wetlands. Background technique [0002] In the process of wetland management and operation, sewage needs to pass through the micro-aeration vertical flow wetland and then enter the horizontal subsurface flow wetland. During this process, the dissolved oxygen in the sewage is relatively high, so it is in an aerobic state in the horizontal subsurface flow wetland. Nitrification is a strictly anaerobic process, oxygen inhibits denitrification enzymes, and oxygen is the preferred electron acceptor during the oxidation of organic matter, thus preventing nitrate and nitrite nitrogen from acting as electron acceptors. [0003] In recent years, researchers have gradually discovered the existence of aerobic denitrifying bacteria, but people have isolated some aerobic denitrifying bacteria in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/34C02F2305/06C02F2209/14C02F2209/16C02F2101/16C12N1/20C02F3/327C02F3/341C02F3/348C02F3/305C12N15/01C12N1/36Y02W10/10Y02E50/30C02F3/1205C02F2209/02C02F2209/06C12Q1/02C02F2209/20C02F2103/007C02F3/28C12N11/08C12N11/02C12Q1/04C12Q1/12C12Q1/24
Inventor 柯凡刁飞李文朝潘继征
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
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