Method for automatically implementing pairing of multiple sets of cells

A technology for grouping cells and cells, which is applied in the field of automatic realization of multi-group cell pairing, and can solve problems such as irrelevant cell particles and collisions

Pending Publication Date: 2019-10-15
NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] In the actual cell operation, there are the following challenging problems: first, cells are randomly distributed in the operation space; second, it is necessary to solve the collision between cells and obstacles and deal with irrelevant cell particles entering the work space; third, Establish automated operations to achieve mass cell movement and pairing operations. At present, there are few research reports on automation to achieve mass cell movement and pairing

Method used

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  • Method for automatically implementing pairing of multiple sets of cells
  • Method for automatically implementing pairing of multiple sets of cells
  • Method for automatically implementing pairing of multiple sets of cells

Examples

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Embodiment Construction

[0019] The invention will be described in further detail below in conjunction with the accompanying drawings.

[0020] 1) Establish a kinetic model for optical tweezers to capture cell movement and pairing. Consider M groups of cells, each group containing N cells. The kinetic equation for capturing the i-th cell (i=1,2...N) in the k-th group (k=1,2...M) by optical tweezers is as follows:

[0021]

[0022] where m ik and q ik are the mass and position of the i-th captured cell in the k-th group, respectively; and are the velocity and acceleration of the cell, respectively; F trap and F drag are the light-harvesting force and viscous resistance, respectively; a ik and b ik are the stiffness coefficient and viscous drag coefficient of the optical tweezers, respectively; u ik are the position coordinates of the focal point of the optical tweezers.

[0023] The multigroup cell movement and pairing control problem is formulated as follows:

[0024] Consider M groups...

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Abstract

The invention discloses a method for automatically implementing pairing of multiple sets of cells, and belongs to the field of operation and control of micro-nano robots. The method comprises the following steps: (1) identifying a target cell, and moving the non-target cell out of a work area; (2) generating optical tweezers on the target cell; (3) redistributing the position of the optical tweezers according to the pairing method; and (4) determining whether the cell moves to the desired position; if so, ending the pairing operation; otherwise, returning to the third step to continue execution. The method disclosed by the invention can automatically implement moving and pairing operations of multiple sets of cells, and implement high-volume, high-precision and high-throughput cell pairingoperations.

Description

technical field [0001] The invention relates to a method for automatically realizing multiple groups of cell pairing, which belongs to the field of micro-nano robot operation and control. Background technique [0002] Moving and matching biological cells or particles from different groups has a wide range of biomedical applications, such as studying the interaction between antigens and CD+8 T cells, studying the reaction between cancer cells and immune cells, cellular immunology, Research on signal transduction mechanism in cell communication, cell fusion, and exploration of organ formation mechanism, etc. All of the above studies involve cell pairing operations. Therefore, pairing biological cells from different groups into desired arrays has a broad clinical application background and has attracted extensive attention. [0003] In the actual cell operation, there are the following challenging problems: first, cells are randomly distributed in the operation space; second,...

Claims

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Application Information

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IPC IPC(8): C12N5/00G16B5/00
CPCC12N5/00G16B5/00Y02D30/70
Inventor 解明扬张瀚
Owner NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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