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Broiler chicken double toxin detoxicant and preparation method thereof

An antidote and toxin technology, which is applied in the field of broiler double toxin antidote and its preparation, can solve problems such as economic loss, endangering animal health, endangering human health, etc., and achieve the effects of reducing accumulation, maintaining stability, and alleviating damage

Inactive Publication Date: 2019-10-11
河南广安生物科技股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, the problem of mycotoxins has plagued most animal husbandry industries. From polluting feed, endangering animal health, and even endangering human health through food, it has brought many adverse effects and caused huge economic losses.
Due to current concerns about single AFB 1 Or there are many studies on the absorption, metabolism and harm of ZEA, but there are few studies on the interaction, superimposed toxicity and harm mechanism of the two toxins at the same time, so it is necessary to do in-depth research

Method used

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  • Broiler chicken double toxin detoxicant and preparation method thereof
  • Broiler chicken double toxin detoxicant and preparation method thereof
  • Broiler chicken double toxin detoxicant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of broiler double toxin antidote, the steps are as follows:

[0022] Cultivation of strains: Inoculate the Bacillus subtilis preserved in the laboratory on LB medium, cultivate at 37°C and 200r / min, and insert into fresh medium at 2% inoculum amount after 24 hours, and then measure the live bacteria after 24 hours of cultivation number. Lactobacillus casei was inoculated on the MRS medium, and cultured at 37°C in a static state. After 24 hours, 2% of the inoculum was added to fresh medium, and the number of viable bacteria was measured after 24 hours of culture. Candida utilis was inoculated on YPD medium, cultivated at 30°C and 200r / min, and after 24 hours, 2% of the inoculum was added to fresh medium, and after another 24 hours of cultivation, the number of viable bacteria was measured, and the bacterial liquid was freeze-dried and stored , the number of live bacteria of Bacillus subtilis in the frozen bacteria powder is 3.0×10 11 CFU / g, the via...

Embodiment 2

[0033] A preparation method of broiler double toxin antidote, the steps are as follows:

[0034] The cultivation of bacterial classification: with reference to embodiment 1

[0035] Preparation of toxin standard: the test system is 5mL, and the blank medium is MRS medium. Control group: 1.975mL normal saline + 3mL MRS medium + 0.025mL AFB 1 Standard product (10mg / L); test group: three kinds of probiotics in different volumes, add 0.025mL AFB 1 Standard product (10mg / L), add 3mL MRS medium, add normal saline to make up 5mL. The design has a total of 18 test points, each test point has 3 repetitions, and each repetition sets AFB 1 The content is 50μg / L.

[0036] Feeding experiment:

[0037] A total of 400 Ross 308 broiler chickens, which were healthy at 1 day old and had no difference in body weight, were selected and divided into 8 treatment groups, with 5 replicates in each treatment group and 10 broilers in each replicate. The experiment was divided into two stages: the...

Embodiment 3

[0040] A preparation method of broiler double toxin antidote, the steps are as follows:

[0041] The cultivation of bacterial classification: with reference to embodiment 1

[0042] Preparation of toxin standard: the test system is 5mL, and the blank medium is MRS medium. Control group: 1.825mL normal saline + 3mL MRS medium + 0.125mL AFB 1 Standard product (2mg / L) + 0.05mL ZEA standard product (50mg / L); test group: three kinds of probiotics in different volumes, adding 0.125mLAFB 1 Standard product (2mg / L) and 0.05mL ZEA standard product (50mg / L), add 3mL MRS medium, add normal saline to make up 5mL. The design has a total of 18 test points, each test point has 3 repetitions, and each repetition sets AFB 1 50μg / L, ZEA 500μg / L.

[0043] Feeding experiment:

[0044] A total of 400 Ross 308 broiler chickens, which were healthy at 1 day old and had no difference in body weight, were selected and divided into 8 treatment groups, with 5 replicates in each treatment group and ...

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Abstract

The invention relates to the field of mycotoxin biological detoxicants, in particular to a broiler chicken double toxin (including aflatoxin B1 and zearalenone) detoxicant and preparation method thereof. after being cultured by a liquid medium, bacillus subtilis, lactobacillus casei and candida utilis are prepared into three kinds of freeze-dried fungus powder through a freeze drying technology. After the three kinds of freeze-dried fungus powder are mixed evenly according to a proportion, the broiler chicken double toxin detoxicant is obtained and stored at the temperature of 4 DEG C. Throughthe researched broiler chicken double toxin detoxicant, the stability of microflora of broiler chicken gastrointestinal tracts can be maintained, mycotoxin is degraded, accumulation of mycotoxin in tissue organs is reduced, damage of mycotoxin to the tissue organs is relieved, the productivity of the broiler chickens and the metabolic rate of nutrient substances are increased, the diarrhea rate and death rate are decreased, and foundations are laid for elimination of mycotoxin harm.

Description

technical field [0001] The invention relates to the field of biological antidotes, in particular to a broiler double toxin antidote and a preparation method thereof. Background technique [0002] In recent years, the problem of mycotoxins has plagued most animal husbandry industries. From polluting feed, endangering animal health, and even endangering human health through food, it has brought many adverse effects and caused huge economic losses. Due to current concerns about single AFB 1 Or there are many studies on the absorption, metabolism and harm of ZEA, but there are few studies on the interaction, superimposed toxicity and harm mechanism of the two toxins at the same time, so it is necessary to do in-depth research. The detoxification methods of mycotoxins are divided into physical methods, chemical methods and biological methods. Now the most suitable method is the biodegradation method, which avoids many shortcomings of the physical and chemical methods. And the B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A61K36/064A61P39/02A61K35/742A61K35/747C12R1/125C12R1/245C12R1/72
CPCA61K35/742A61K35/747A61K36/064A61P39/02C12N1/16C12N1/20A61K2300/00
Inventor 尹清强王涛高天增常娟王平党晓伟宋安东刘超齐李茂龙白献晓周璞
Owner 河南广安生物科技股份有限公司
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