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The performance of dragging silk and its silk protein gene sequence

A protein and nucleotide sequence technology, applied in genetic engineering, plant gene improvement, peptide source, etc., can solve the problems of sporadic, limited spider silk protein gene resources, etc.

Active Publication Date: 2021-01-26
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently available spidroin gene resources are very limited, especially long sequence resources with more in-depth analysis significance
In addition, the existing studies on domain functions are mainly concentrated on a few species, and the research is relatively sporadic. It is urgent to continuously explore new excellent spider and spider silk protein resources.

Method used

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  • The performance of dragging silk and its silk protein gene sequence
  • The performance of dragging silk and its silk protein gene sequence
  • The performance of dragging silk and its silk protein gene sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Protein Sequence and Gene Sequence of Towed Silk MaSp

[0025] MaSp (Major ampullate silk, main ampullate silk), also known as traction silk, is composed of MaSp1 and MaSp2. MaSp1 does not contain proline, and its main function is to escape, weave the net, weave the net frame, and form the frame silk of the net skeleton. Has high strength. At present, only one kind of masp1 (439 aa) has been published in the genus Arachnid (Orbidae), and there are also masp1 (945 aa) and masp2 (3360) of the golden bead of the genus Aurora. , other relative species such as Masp2 (2466 aa), MaSp1 (3123 aa) and MaSp2 (3779 aa) of the black widow spider, the other sequences were too short to evaluate their genes feature.

[0026] Through sequencing, we discovered the amino acid sequence of MaSp1 in the towed silk of P. arachnid as shown in SEQ ID NO: 1, a total of 938 amino acids, and the amino acid sequence of MaSp2 is shown in SEQ ID NO: 2, a total of 642 amino acids. From th...

Embodiment 2

[0039] The mechanical property analysis of embodiment 2 cloud spot spider pulling silk

[0040] Method: The instron tensile machine was used to test the mechanical properties of the traction wires of various spiders (Golden Bead, New Woman, Cloud Spotted Spider, Acanthus spinosa). Test conditions: temperature: 24-26°C, humidity: 75-90% , The spinning speed is 10.57cm / s. At the same time, use a scanning electron microscope (S4800 1.0kV 6.6mm×9.00k SE(U)) to measure the diameter of the spider silk, according to stress=P / A, strain=L-L0 / L0 (P is the load, A is the initial cross-sectional area of ​​the spider silk ), each spider silk sample was tested multiple times, and the statistical processing of the data was mainly completed using origin2018 software, and finally the mechanical data of spider silk under different tensile strengths were obtained.

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Abstract

The present invention discloses the drag silk performance of the cloud spider and its silk protein gene sequence. The silk protein amino acid sequence is shown in SEQ ID NO: 1 or / and SEQ ID NO: 2, or its amino acid sequence is shown in SEQ ID NO: 1 Or / and the amino acid sequence obtained by the polypeptide described in SEQ ID NO: 2 through substitution, deletion or addition of one or several amino acid residues and having equivalent mechanical properties. The present invention discovers the protein sequence and gene sequence of the drag silk proteins MaSp1 and MaSp2 of the cloud spider for the first time, and finds that the drag silk of the cloud spider has excellent mechanical properties and very strong toughness, which is significantly better than that of other spider species. The present invention lays the foundation for further understanding the evolution pattern of spidroin proteins of different species and different properties, and lays an important theory for the development of super spidroin proteins.

Description

technical field [0001] The present invention relates to cloud spot spider pulling silk and its silk protein gene sequence. Background technique [0002] Spider silk is a kind of natural silk fiber with excellent strength, good damping property and outstanding elasticity in nature. It is known as "biological steel" and is a kind of biomass material with great application potential. Especially in military industry, medicine and other fields. However, the research and development of new silk protein materials is still relatively backward, lacking independent intellectual property resources. Spider silk is a kind of biomass material with great application potential in nature. There are many types and quantities of spider silk, and their structural characteristics are different. However, the current research mainly focuses on individual species, and the understanding of the structure and properties of spider silk proteins is still unclear. deep. The current dilemma is that the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C12N15/12
CPCC07K14/43518
Inventor 朱克森相辉陈新董庆林周世怡高雷叶杰辉
Owner SOUTH CHINA NORMAL UNIVERSITY
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