A protective solution for transporting animal cells at room temperature, its preparation method and application

A technology of animal cells and protective fluid, which is applied in the field of biomedicine to achieve the effect of convenient use

Active Publication Date: 2021-10-29
中源和美(广州)生命医学研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of this cell protection solution is limited to mesenchymal stem cells, and low molecular weight heparin calcium is added to the protection solution, which will bring certain risks to patients with coagulation disorders in clinical use

Method used

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  • A protective solution for transporting animal cells at room temperature, its preparation method and application
  • A protective solution for transporting animal cells at room temperature, its preparation method and application
  • A protective solution for transporting animal cells at room temperature, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1 Compound amino acid mother liquor

[0114] The composition of compound amino acid mother liquor is as follows, wherein every 1000ml contains: L-proline (C 5 h 9 NO 2 ) 1.00g, L-serine (C 3 h 7 NO 3 ) 1.00g, L-alanine (C 3 h 7 NO 2 ) 2.00g, L-isoleucine (C 6 h 13 NO 2 ) 3.52g, L-leucine (C 6 h 13 NO 2 ) 4.90g, L-aspartic acid (C 4 h 7 NO 4 ) 2.50g, L-tyrosine (C 9 h 11 NO 3 ) 0.25g, L-glutamic acid (C 5 h 9 NO 4 ) 0.75g, L-phenylalanine (C 9 h 11 NO 2 )5.33g, L-arginine hydrochloride (C 6 h 14 N 4 o 2 HCl) 5.00g, L-lysine hydrochloride (C6H 14 N 2 o 2 HCl) 4.30g, L-valine (C 5 h 11 NO 2 ) 3.60g, L-threonine (C 4 h 9 NO 3 ) 2.50g, L-histidine hydrochloride (C 6 h 9 N 3 o 2 HClH 2 O) 2.50g, L-tryptophan (C 11 h 12 N 2 o 2 ) 0.90g, L-methionine (C 15 h 11 NO 2 S) 2.25g, L-cystine (C 6 h 12 N 2 O4S 2 ) 0.10g, glycine (C 2 h 5 NO 2 ) 7.60g, sorbitol (C 6 h 14 o 6 )50.00g, sodium bisulfite (NaHSO 3 )0.5g. ...

Embodiment 2

[0119] Example 2 Preparation of protective solution

[0120] Dilute the compound amino acid mother liquor prepared in Example 1 into a compound amino acid dilution solution with a total amino acid concentration of 30 mg / ml.

[0121] Animal cell protection solution A is composed of the following components, wherein the concentration of each component is: glucose 40mg / ml, human albumin injection 15mg / ml, calcium chloride 0.02mg / ml, potassium chloride 0.02mg / ml, Sodium chloride 6mg / ml, sodium bicarbonate 3mg / ml, the balance is compound amino acid dilution (compound amino acid concentration is 30mg / ml).

[0122] The preparation method of animal cell protection liquid A comprises the following steps:

[0123] Dissolve glucose, human serum albumin, calcium chloride, potassium chloride, sodium chloride, and sodium bicarbonate in the compound amino acid diluent, fill up to 100ml with the compound amino acid diluent, and make a composition with the concentration of the above compone...

Embodiment 3

[0124] Example 3 Preparation of protective solution

[0125] Animal cell protection solution B is composed of the following components, wherein the concentration of each component is: glucose 50mg / ml, human albumin injection 20mg / ml, calcium chloride 0.03mg / ml, potassium chloride 0.03mg / ml, Sodium chloride 5mg / ml, sodium bicarbonate 4mg / ml, surplus is the compound amino acid mother liquor of embodiment 1 (compound amino acid concentration is 50mg / ml).

[0126] The preparation method of animal cell protection liquid B comprises the following steps:

[0127] Glucose, human serum albumin, calcium chloride, potassium chloride, sodium chloride, sodium bicarbonate are dissolved in compound amino acid mother liquor, make up to 100ml with compound amino acid mother liquor, be made into 2 ℃~37℃ animal cell protection solution.

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Abstract

The invention relates to a protective liquid for transporting animal cells at room temperature, a preparation method and application thereof. The protective solution contains amino acids, glucose, albumin, calcium chloride, potassium chloride, sodium chloride and sodium bicarbonate. The animal cell protection solution is prepared from medical materials, without heat source and without chemical addition. It can realize routine express transportation of cells at 2℃~37℃ for 72 hours, and the cell survival rate can reach more than 85%. Using the animal cell protection solution of the present invention to transport cells does not need to be thawed, re-screened, cultured, or washed, and can be directly used in clinical or animal experiments.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a protective solution for transporting animal cells at normal temperature, its preparation method and application. Background technique [0002] In recent years, cell therapy technology has developed rapidly. Since various cell therapy techniques require appropriate treatment of target cells in vitro, these operations are required to be completed in a GMP environment, and usually have certain technical difficulties and take a certain amount of time, so the preparation of therapeutic cells is difficult to complete in the hospital, usually by The cell preparation laboratory is ready and shipped to the hospital for use. [0003] In addition to the cell preparation technology itself, the factors affecting the effect of cell therapy, the transportation link may have a greater impact on the viability and function of cells. Especially when some cell therapy products may...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/0775
CPCC12N5/0656C12N5/0668C12N2500/12C12N2500/14C12N2500/32C12N2500/34C12N2501/998
Inventor 高文勇邓鹏
Owner 中源和美(广州)生命医学研究有限公司
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