Magnetic heat triggered cascaded enzyme reaction supermolecular gel and preparing method and application thereof
A technology of supramolecular gel and triggering cascade, which is applied in the field of biological and medical nanomaterials, can solve the problems of short treatment time and damage to surrounding normal tissues, and achieve the effect of simple operation
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Embodiment 1
[0036] Preparation of Glucose-loaded PLGA Nanocapsules by Double Emulsion Solvent Evaporation Method
[0037] 3 mg of glucose was added to 250 microliters of ultrapure water, ultrasonically dissolved and fully mixed, and used as the internal water phase; 300 mg of PLGA11K-MPEG5K powder was added to 5 ml of chloroform, ultrasonically dissolved, and used as the oil phase; prepare 1.0% (w / v, g / mL) PVA solution 15mL as the external aqueous phase. Add the inner water phase to the oil phase, and ultrasonicate for 30 s in an ice bath at 80W to obtain colostrum. Add colostrum into 15ml of 1% PVA, and ultrasonicate for 2 minutes in an 80W ice bath to form a water-in-oil-in-water double emulsion system. The mixed solution was added into 250ml of 0.3% PVA, stirred at 500r / min for 3h, and the chloroform was slowly volatilized. Collect the reaction solution, centrifuge and wash twice at 10,000 r / min to remove excess PVA, dilute to 1.5 mL with pure water and store at 4°C.
[0038] fig...
Embodiment 2
[0040] Preparation of oxygen-carrying PFOB nanoemulsion by ultrasonic dispersion
[0041] Add 20ml of ultrapure water into a 100mL three-necked bottle as the water phase, add 300 mg of DSPE-MPEG2000 as a surfactant into the water phase, heat the water phase with an oil bath to raise the temperature to 100°C, and heat it at a rate of 600r / min Stir, then add 0.9mL PFOB dropwise as the oil phase, turn off the heat source, continue stirring for 30min to obtain nanoemulsion, condense to 1.5mL volume by ultrafiltration with a 30K ultrafiltration tube, and store at 4°C.
[0042] figure 2 b is negative staining image of PFOB oxygen-carrying nano-emulsion in transmission electron microscope, the nano-emulsion is spherical, the size is between 30-50 nm, and the dispersion is good.
Embodiment 3
[0044] Preparation of Iron Tetroxide Nanoparticles by High Temperature Pyrolysis and Their Surface PEI Modification
[0045] The experimental device is a 100 ml inclined three-necked flask and a temperature program control device. Add 2 mmol of iron precursor iron acetylacetonate, 20 mL of reaction solvent dibenzyl ether, and a total of 12 mmol of surfactants oleic acid and oleylamine into the three-necked flask. The programmed temperature raised the temperature of the reaction system to 220°C at a heating rate of 3.3°C / min, and the nucleation of nanoparticles lasted for one hour. After that, the temperature was still raised to 290°C at a heating rate of 3.3°C / min for 30 minutes. During the experiment, nitrogen gas was continuously fed to remove oxygen in the system and play a stirring role, while condensing and refluxing. After the reaction is completed, remove the heat source, transfer the reaction system to a beaker after it is naturally cooled to room temperature, and wash...
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