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Preparation and application method of material for selective separation and enrichment of pterin compounds

A compound and chloride technology is applied in the field of preparation and application of materials for selective separation and enrichment of pterin compounds. The process is simple, the adsorption and extraction efficiency is improved, and the energy consumption is saved.

Active Publication Date: 2022-03-11
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption of these stationary phases is based on the dense pores on the surface of the particles or the large specific surface area. The adsorption / desorption process of the target involves mass transfer and diffusion inside and outside the micropores. The adsorption efficiency has limiting factors. Adsorption and desorption The speed is slow, and usually several column bed volumes of eluent are required to elute the target site from the column, and further steps such as heating and nitrogen blowing are required to further concentrate the target substance. The operation process is very cumbersome and time-consuming, heating, nitrogen blowing, etc. The operation will also destroy the structure of the target object, and it is not the best choice for the pretreatment of unstable pterin polymers

Method used

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  • Preparation and application method of material for selective separation and enrichment of pterin compounds
  • Preparation and application method of material for selective separation and enrichment of pterin compounds
  • Preparation and application method of material for selective separation and enrichment of pterin compounds

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preparation example Construction

[0047] A method for preparing a functionalized composite nanofiber material capable of selectively separating and enriching pterin compounds in complex samples according to the present invention comprises the following steps:

[0048] Step 1, adding the high molecular polymer into solvent A, after it dissolves, put it on a constant temperature magnetic stirrer and mix evenly, then place the solution at room temperature and continue to stir for 12-20h to obtain a high molecular polymer solution ; adding the solution to the injection needle of the electrospinning device, and injecting the solution onto the placed receiving screen through the injection device for electrospinning to obtain the polymer nanofiber core layer;

[0049] Step 2, performing injection electrospinning of the above-mentioned high molecular polymer solution to obtain a high molecular polymer nanofiber core layer;

[0050] Step 3, after diluting 30wt% polyetherimide high polymer aqueous solution to 0.83wt% wi...

Embodiment 1

[0068] Methotrexate (MTX) and its glutamic acid polymer (MTTXPG) in the urine sample of embodiment 1 n ) rapid extraction and separation of compounds

[0069] The collection of most biological samples is very difficult and precious and rare. Traditional methods generally require a large sample volume, at least at the milliliter level, and the operation process is very complicated, involving a series of steps such as nitrogen blowing and evaporation to dryness. These time-consuming and labor-intensive operations are very unfavorable for the detection of biological samples. Because the amount of biological samples is scarce, and the pterin compounds in them are very small, the multi-step and long-term operation will easily cause the loss of samples and the damage and loss of target objects. In this example, a composite electrospun nanofiber material functionalized with Pd(II) was prepared and applied to solid phase extraction technology as an adsorbent. The high specific surfa...

Embodiment 2

[0081] Glutamic acid polymer (MTTPG) of methotrexate in the red blood cell sample of embodiment 2 n ) rapid extraction and separation of compounds

[0082] Step 1: Obtaining blood red blood cell sample processing solution: take 0.5 mL of blood, centrifuge it at 3000 r / min for 5 min as soon as possible, discard the supernatant, leave the precipitate, and obtain a blood red blood cell sample, take 100 μl blood red blood cell sample, add 300 μl aqueous solution or the standard solution, shake and mix well, then add 400 μl of acetonitrile to shake for 30 seconds, centrifuge at 3000 r / min for 5 minutes, take the supernatant, and remove the precipitate. Add 1ml of chloroform solution to the supernatant again, shake for 30s, centrifuge at 3000r / min for 5min, take the supernatant, and obtain the red blood cell sample processing solution.

[0083] Step 3: Use solid-phase extraction materials to process the standard solution or treatment solution: a total of 400 μl of the treatment sol...

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Abstract

The invention discloses a composite nanofiber material capable of selectively separating and enriching pterin targets in samples with complex backgrounds, a preparation method and an application method thereof. The composite nanofiber material consists of an electrospun nanofiber core layer, a modification layer I and The composition of the modification layer II; at the same time, it also discloses the preparation method of the composite nanofiber material, and also discloses the method for selectively separating and enriching pterin compounds in complex samples by using the above composite nanofiber material. The functionalized nanofibers successively modified by the modification solution I and the modification solution II have selective adsorption for pterin substances in complex samples, and can be applied in the extraction and detection of pterin compounds.

Description

technical field [0001] The invention relates to an extraction method based on functionalized composite nanofibers, in particular to a composite nanofiber material capable of selectively separating and enriching pterin compounds, its preparation method, and the application of the composite nanofiber material to complex samples. A method for selective separation and enrichment of pterin compounds. Background technique [0002] Pterin refers to the general name of pteridine derivative 2-amino-4-carbonylpteridine or its derivatives with two nitrogen rings. Pterin substances are biologically active substances in the human body, and some pterin substances are used as drugs in clinical practice. This results in the possibility of pterins being present in both body fluids and the environment. Studies have shown that in the human body, there are relatively large differences in blood table and urine pterin levels between healthy people and patients with various diseases. The resear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/286B01J20/30G01N1/34G01N1/40B01D15/20B01D15/42
CPCB01J20/286B01D15/20B01D15/426G01N1/34G01N1/405
Inventor 康学军谢丽
Owner SOUTHEAST UNIV
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