Biotechnology breeding method of regenerated functional rice
A biotechnology and functional rice technology, which is applied in the field of rice breeding, can solve the problems of increasing rice yield and the difficulty of rice planting area, and achieve the effect of increasing yield, not growing high, and having strong growth ability
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[0031] Embodiment 1 of the present invention: a biotechnology breeding method for regenerative functional rice, comprising the following steps: S1: selecting barnyardgrass as the donor; S2: selecting Xiangwanxian No. 5 as the parent; S3: extracting the DNA of the donor ; S4: Introduce the DNA of the donor to select Xiangwanxian 5; S5: Collect the distant hybrid seeds of Xiangwanxian 5 in step S4; Step S6: Select and breed the distant hybrid seeds collected in step S5 for seven generations Obtain seeds of new lines with stable growth.
[0032] The method for extracting DNA comprises the following steps:
[0033] S31: Weigh 0.2 g of the first to fourth young leaves of the donor seedling, remove the DNA contamination on the surface, put them in a mortar and grind them together with liquid nitrogen to form a fine powder;
[0034] S32: Transfer the fine powder of the donor into a 2ml centrifuge tube, immediately add 1000 μl of CTAB extract preheated to 65°C, incubate at 65°C for 3...
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