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Method for extracting collagen from silver carp skins by ultrasonic acid pretreatment assisted acid-enzyme method

A fish skin collagen and acid pretreatment technology, which is applied to the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of waste of resources and low extraction rate, and achieve convenient nutrition, simple operation and stability. low sex effect

Active Publication Date: 2019-09-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to invent a simple and efficient method for extracting collagen from the leftover fish skin of the silver carp industry, solve the problem of waste of resources in the leftovers of fish product processing and production, and overcome the traditional collagen extraction method. The shortcoming of low efficiency; the technical method of the present invention is simple and easy, and extraction efficiency is high, and about acid method, enzymatic method and ultrasonic pretreatment combine the research method of extracting collagen protein and have not yet been reported

Method used

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  • Method for extracting collagen from silver carp skins by ultrasonic acid pretreatment assisted acid-enzyme method
  • Method for extracting collagen from silver carp skins by ultrasonic acid pretreatment assisted acid-enzyme method
  • Method for extracting collagen from silver carp skins by ultrasonic acid pretreatment assisted acid-enzyme method

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Effect test

Embodiment 1

[0036] (1) Pretreatment of raw materials:

[0037] Recover the leftovers from the leftovers of the fish ball factory, pick out the fish skin, wash and cut into pieces (1cm×1cm), freeze-dried and then use it directly, or store it at -20°C for later use; take the freeze-dried fish skin and store it In 0.1mol / L NaOH solution, the mass volume ratio of freeze-dried fish skin to NaOH solution is 1:25; soak for 6 hours after homogeneous stirring for 5 minutes, replace the lye every 2 hours during the soaking period, remove impurities and pigments; use after soaking Rinse with distilled water until the pH is neutral, then soak the filtered fish skin again in isopropanol solution for degreasing, wherein the mass volume ratio of filtered fish skin to isopropanol solution is 1:10, and the degreasing time is 9 hours During the degreasing period, the isopropanol solution was replaced every 3 hours; after degreasing, it was centrifuged at 10000g for 30min at 4°C to obtain the pretreated fis...

Embodiment 2

[0042] (1) Pretreatment of raw materials:

[0043]Recover the leftovers from the leftovers of the fish ball factory, pick out the fish skin, wash and cut into pieces (1cm×1cm), freeze-dried and then use it directly, or store it at -20°C for later use; take the freeze-dried fish skin and store it In 0.1mol / L NaOH solution, the mass volume ratio of freeze-dried fish skin to NaOH solution is 1:25; soak for 6 hours after homogeneous stirring for 5 minutes, replace the lye every 2 hours during the soaking period, remove impurities and pigments; use after soaking Rinse with distilled water until the pH is neutral, then soak the filtered fish skin again in isopropanol solution for degreasing, wherein the mass volume ratio of filtered fish skin to isopropanol solution is 1:10, and the degreasing time is 9 hours During the degreasing period, the isopropanol solution was replaced every 3 hours; after degreasing, it was centrifuged at 10000g for 30min at 4°C to obtain the pretreated fish...

Embodiment 3

[0048] (1) Pretreatment of raw materials:

[0049] Recover the leftovers from the leftovers of the fish ball factory, pick out the fish skin, wash and cut into pieces (1cm×1cm), freeze-dried and then use it directly, or store it at -20°C for later use; take the freeze-dried fish skin and store it In 0.1mol / L NaOH solution, the mass volume ratio of freeze-dried fish skin to NaOH solution is 1:25; soak for 6 hours after homogeneous stirring for 5 minutes, replace the lye every 2 hours during the soaking period, remove impurities and pigments; use after soaking Rinse with distilled water until the pH is neutral, then soak the filtered fish skin again in isopropanol solution for degreasing, wherein the mass volume ratio of filtered fish skin to isopropanol solution is 1:10, and the degreasing time is 9 hours During the degreasing period, the isopropanol solution was replaced every 3 hours; after degreasing, it was centrifuged at 10000g for 30min at 4°C to obtain the pretreated fis...

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Abstract

The invention belongs to the field of food bio-utilization and relates to a method for extracting collagen from silver carp skins by an ultrasonic acid pretreatment assisted acid-enzyme method. The method comprises the following steps: reclaiming leftovers from a fish ball mill, picking out fish skins, performing washing, cutting-up and freeze-drying, sequentially soaking the fish skin in NaOH and isopropyl alcohol solutions, and removing protein, pigment and fat; and then, soaking the pretreated fish skins in lactic acid, performing ultrasonic acid-lactic acid pretreatment in an ultrasonic pond, adding pepsase into the mixed solution, performing acid-enzyme extraction, performing centrifuging to obtain a collagen crude extract; adding NaCl into the collagen crude extract to perform salting-out, performing centrifuging, dissolving the precipitate in an acetic acid solution, performing dialysis sequentially with an acetic acid solution and distilled water, and performing vacuum freeze drying to obtain collagen. The method mainly solves the problem resource waste of leftovers of fish product processing production, and overcomes the defect of low extraction rate of a traditional collagen extraction method; and the dialysis freeze-drying is represented in natural I-type collagen, and the extraction rate is 75.8 percent.

Description

technical field [0001] The invention belongs to the field of biological utilization of food, and in particular relates to a method for extracting collagen from silver carp skin with the aid of ultrasonic acid pretreatment and acid enzymatic method. Background technique [0002] At present, the main raw material for collagen preparation is the skin or bone of terrestrial mammals, such as pigs, cattle and chickens, but with the outbreak of diseases such as mad cow disease and foot-and-mouth disease and some religious restrictions, the collagen of terrestrial mammals is restricted Security Concerns and Restrictions on Use. Compared with animal processing and eating habits, aquatic product processing has more waste materials and has more resource advantages, and the collagen of aquatic animals is more likely to be affected by environmental conditions such as enzymes, temperature, and pH, that is, the stability of aquatic collagen is relatively low. Low, easy for nutrition, dige...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/14C07K1/30
CPCC07K14/78
Inventor 周存山杨璇余筱洁马海乐夏蓉陶汇源
Owner JIANGSU UNIV
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