Cell size measurement device of lensless imaging system and measurement method of measurement device

Abstract

The invention discloses a cell size measurement device of a lensless imaging system. The device comprises a light support, wherein a light source and a convex lens are fixed to the light support in the vertical direction, the light source is located over the convex lens, a CMOS sensor is arranged under the convex lens, and the CMOS sensor is connected with a PCB. Through the device, sampling is clear, and leading disturbance of diffraction on cell size measurement is avoided. The invention further discloses a measurement method of the cell size measurement device of the lensless imaging system. According to the method, the cell size measurement device of the lensless imaging system is adopted for measurement, imaging data of a to-be-detected cell sample is exported, cells and diffraction rings thereof in the to-be-detected cell sample are located and labeled, intact radius data of the labeled cells is obtained through measurement and calculation, and size measurement of the cells is completed. Through the measurement device and the measurement method, the cell size in the to-be-detected sample can be subject to real-time measurement and statistical analysis.

Description

technical field [0001] The invention belongs to the technical field of cell size measurement, relates to a cell size measurement device of a lensless imaging system, and also relates to a measurement method of the cell size measurement device of the lensless imaging system. Background technique [0002] Traditional cell detection is mainly done by light microscope. This method has a low degree of automation, large differences in results, and cannot complete cell count statistics under high-throughput conditions, which is not conducive to portable applications. The use of flow cytometry has improved the automation of cell detection to a certain extent. But its equipment is huge, the operation is complicated, and the price is expensive. In 2006, Yang changhuei's research group at California Institute of Technology first proposed the concept of a lensless optofluidic microchip. The advantage of the lensless imaging device is that it does not require lens imaging, and the sam...

AI Technical Summary

Problems solved by technology

[0003] Due to the use of lens-free shadow imaging, diffraction phenomenon inevitably occurs, and the diffraction halo formed by diffraction makes it impossible to determine the real cell size

The existing diffraction recovery method adopts the holographic recovery method. Due to many interference factors, it cannot remove the interference caused by diffraction to the cell size measurement in engineering implementation, and the holographic imaging method is complicated, costly, and time-consuming.

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