Microfluidic chip component capable of realizing digital PCR (polymerase chain reaction) quickly and application thereof

Abstract

The invention provides a microfluidic chip component capable of realizing digital PCR (polymerase chain reaction) quickly and application thereof. The microfluidic chip component comprises at least one microfluidic chip, a radiator arranged below the microfluidic chips and a heater arranged above the microfluidic chips, a semiconductor refrigerator and a heat conduction plate arranged above the same are arranged between the radiator and the microfluidic chips, and a thin film layer is bonded at the bottoms of the microfluidic chips and is in butt connection with the heat conduction plate. Liquid drop generation and PCR on a same microfluidic chip are realized, and transfer after liquid drop generation in the prior art is avoided; by bonding the thin film layer, heat capacity of a heated object is reduced, so that quick temperature increase and decrease is realized.

Description

technical field [0001] The invention belongs to the technical field of high-throughput analysis systems, and in particular relates to a rapid realization of PCR reaction, especially a microfluidic chip component applied to digital PCR and an integrated device using the component. Background technique [0002] Microfluidics is a technology for precise control and manipulation of microscale fluids, which has the characteristics of small capacity (nanoliter, picoliter, femtoliter level) and low energy consumption. People use microfluidic technology to integrate the basic operation units such as sample preparation, reaction, separation and detection in the process of biological, chemical and medical analysis into the microfluidic chip with microfluidic pipeline as the basic structure, realizing rapid sample processing and It has the advantages of less detection, reagent and sample usage. Due to its great potential in the fields of biology, chemistry, and medicine, it has develo...

AI Technical Summary

Problems solved by technology

However, this system has the following disadvantages: 1) After the system produces droplets, the user needs to manually transfer the droplets to the 96-well plate to proceed to the next PCR reaction, which will cause loss or rupture of the droplets; 2) due to The heat transfer rate is limited (the sample volume in the test tube is thicker, and the heating starts from the outside of the test tube), and the reaction speed of PCR in the test tube is relatively slow, so the entire PCR process takes a long time, generally takes 150 minutes

Because the high-throughput setting is to process many samples at the same time, the samples are usually placed in a 96- or 384-well plate, and the area that needs to be controlled is large, so the heat capacity of the sample object that needs to be heated and cooled is very high (need to store the 96 or 384 wells of the sample at the same time. orifice plate for temperature rise and fall), it is difficult to achieve rapid temperature rise and fall

At present, there is no PCR instrument specially designed for microfluidic chips on the market

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