Aflatoxin surface imprinted polymer based on HKUST-1 and application of polymer

Abstract

The invention belongs to the field of molecularly imprinted polymers and discloses an aflatoxin surface imprinted polymer and application thereof in crop detection. The surface imprinted polymer HKUST-1@MIPs is synthesized by using a metal organic framework material HKUST-1 as a carrier and 7-acetoxy-4-methylcoumarin as a substitute template of aflatoxin. The synthesized polymer is used as an adsorbent to prepare a surface imprinting solid-phase extraction column to extract and separate aflatoxin in food, and correspondingly a rapid, efficient and cheap aflatoxin sample pretreatment method isobtained; a detection method of aflatoxin in food, which is low in price, simple in operation and low in detection limit, is developed in combination with high performance liquid chromatography. The pretreatment development of crop toxin detection samples is facilitated, and the problems of high cost and large time consumption of current pretreatment methods are solved.

Description

technical field [0001] The invention belongs to the field of molecularly imprinted polymers, and relates to an aflatoxin surface imprinted polymer and its application in the detection of crops. Background technique [0002] During the process of growth, harvesting, storage and processing, crops are susceptible to fungal infection and produce aflatoxins (Aflatoxins, AFs). Some biological systems and organs in the human body have serious toxic and carcinogenic effects. The AFs produced by the metabolism of Aspergillus flavus will remain in the agricultural products. If the agricultural products are processed into food, they will cause damage to the human body after human consumption; if the agricultural products are processed into feed to feed livestock, AFs will accumulate in the livestock. When people use meat Such products are still at risk from AFs. There are many types of AFs, and there are some differences in the structures of different types of AFs. Among them, aflato...

AI Technical Summary

Problems solved by technology

[0013] Although the combination of molecular imprinting and solid phase extraction can effectively extract target molecules, traditional molecular imprinting polymers also have some disadvantages: it is not easy to completely elute the template molecules; the mass transfer rate is low; the shape of the polymer is irregular; Lower adsorption capacity, etc. (Wang Y, Yang Y, Lan X, et al.Bisphenol A sensing based on surface molecularly imprinted, ordered mesoporous silica[J], ElectrochimicaActa,2011,56(5):2105-2109.; Hongliang H, Xiaoli G, Liying S, et al. Molecularly imprinted polymers based on SBA-15 for selective solid-phase extraction of baicalein from plasma samples[J], Analytical and Bioanalytical Chemistry, 2015, 407(2):509-519.), while surface imprinted Technology can well make up for the above shortcomings

At present, there is no use of metal-organic framework materials as carriers to prepare surface molecularly imprinted polymers for the detection of wheat toxins and other crops

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