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Preparation method and application of a degradable material capable of inducing osteogenic differentiation and bone regeneration

A technology of osteogenic differentiation and degradable materials, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problems of osteoinductive effect accompanied by infection, limited sources, etc., achieve good osteogenic differentiation function, high biological activity, promote The effect of cell adhesion

Active Publication Date: 2022-02-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although autologous bone transplantation does not produce immune rejection and has good osteoinductive and osteoconductive properties, its source is extremely limited; although the source of allograft bone and xenograft bone is abundant, it will cause immune rejection and is accompanied by infection and other complications and poor osteoinductive effect

Method used

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  • Preparation method and application of a degradable material capable of inducing osteogenic differentiation and bone regeneration
  • Preparation method and application of a degradable material capable of inducing osteogenic differentiation and bone regeneration
  • Preparation method and application of a degradable material capable of inducing osteogenic differentiation and bone regeneration

Examples

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Effect test

Embodiment 1

[0036] Example 1 The preparation method of the degradable material loaded with micro RNA-214 inhibitor of the present invention comprises the following steps:

[0037] Step 1: Synthesis of Graphene Oxide-Polyethyleneimine (GO-PEI)

[0038] Graphene oxide (GO) was prepared into 1 mg / mL ultrasonic treatment for later use, and then polyethyleneimine (25k) (PEI) was prepared into 1 mg / mL solution and slowly added to the GO solution within 10 min. The GO-PEI complex solution was obtained by mixing the GO solution with the diluted PEI solution at a mass ratio of 1:2, then sonicating for 15 min, stirring overnight for 12 hours, and then removing free PEI by centrifugation and washing.

[0039] Step 2: Synthesis of graphene oxide-polyethyleneimine-loaded micro-RNA-214 inhibitor complex (GO-PEI-microRNA-214)

[0040]The GO-PEI complex solution and the micro RNA-214 inhibitor were compounded at a mass ratio of 30:1 in phosphate buffer, vortexed for 60 s at room temperature, and incubat...

Embodiment 2

[0045] Example 2 The preparation method of the degradable material loaded with micro RNA-214 inhibitors of the present invention comprises the following steps:

[0046] Step 1: Synthesis of Graphene Oxide-Polyethyleneimine (GO-PEI)

[0047] Graphene oxide (GO) was prepared into 0.1 mg / mL ultrasonic treatment for later use, and then polyethyleneimine (25k) (PEI) was prepared into 0.1 mg / mL solution and slowly added to the GO solution within 10 min. The GO-PEI complex solution was obtained by mixing the GO solution with the diluted PEI solution at a mass ratio of 1:1, then sonicating for 15 min, stirring overnight for 12 hours, and then removing free PEI by centrifugation and washing.

[0048] Step 2: Synthesis of graphene oxide-polyethyleneimine-loaded micro-RNA-214 inhibitor complex (GO-PEI-microRNA-214)

[0049] The GO-PEI complex solution and the micro RNA-214 inhibitor were compounded at a mass ratio of 100:1 in phosphate buffer, vortexed for 60 seconds at room temperature...

Embodiment 3

[0054] Example 3 The preparation method of the degradable material loaded with micro RNA-214 inhibitors of the present invention comprises the following steps:

[0055] Step 1: Synthesis of Graphene Oxide-Polyethyleneimine (GO-PEI)

[0056] Graphene oxide (GO) was prepared at 6 mg / mL and ultrasonically treated for later use, and then polyethyleneimine (25k) (PEI) was prepared at 6 mg / mL and slowly added to the GO solution within 10 min. The GO-PEI complex solution was obtained by mixing the GO solution with the diluted PEI solution at a mass ratio of 1:4, then sonicating for 15 min, stirring overnight for 12 hours, and then removing free PEI by centrifugation and washing.

[0057] Step 2: Synthesis of graphene oxide-polyethyleneimine-loaded micro-RNA-214 inhibitor complex (GO-PEI-microRNA-214)

[0058] The GO-PEI complex solution and the micro RNA-214 inhibitor were compounded at a mass ratio of 60:1 in phosphate buffer, vortexed for 60 seconds at room temperature, and incuba...

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Abstract

The invention discloses a micro RNA-214 inhibitor-loaded degradable material capable of inducing osteogenic differentiation and bone regeneration, which is composed of the following raw materials: graphene oxide, polyethyleneimine, micro RNA-214 inhibitor, silk fibroin , Nano-hydroxyapatite. The micro RNA-214 inhibitor-loaded degradable material with the function of inducing osteogenic differentiation and bone regeneration of the present invention can be degraded in vivo, has better osteogenic differentiation function and higher biological activity, and can promote cell proliferation and differentiation, promote It has obvious advantages in cell adhesion and other aspects, and the degradation products have no obvious impact on the surrounding environment. Therefore, the microRNA‑214 inhibitor-loaded degradable material capable of inducing osteogenic differentiation and bone regeneration of the present invention is suitable for clinical minimally invasive bone repair applications.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method and application of a degradable material capable of inducing osteogenic differentiation and bone regeneration. Background technique [0002] At present, bone defect has become the most common clinical disease. For patients with large bone defects, it is often difficult to achieve self-healing effects by relying on the regeneration and repair ability of the bone itself due to factors such as damage or repair environment. Substitute materials are a common method for repairing large area bone defects and have broad application prospects. Bone tissue engineering is a promising approach for the design of bone substitutes / regenerated new bone tissue for the repair of fractures caused by traffic accidents, traumatic injuries, skeletal diseases, tumor resection, osteonecrosis or rheumatic diseases Appropriate technology is provided. However, bone subs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/54A61L27/22A61L27/12A61L27/08A61L27/18
CPCA61L27/50A61L27/54A61L27/227A61L27/12A61L27/08A61L27/18A61L2430/02A61L2300/432A61L2300/412C08L89/00C08L79/02
Inventor 欧玲伶郭瑞孙挺张晔周志迎刘敏义
Owner JINAN UNIVERSITY
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