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A method for separating heparan sulfate and dermatan sulfate in heparin

A technology of heparan sulfate and dermatan sulfate, which is applied in the field of medicine, can solve problems such as the inability to separate heparan sulfate and dermatan sulfate in heparan or precise quantitative control, and achieve the effect of easy amplification, avoiding loss, and simple process

Active Publication Date: 2021-05-07
SUZHOU WISMED PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the content of chondroitin sulfate and dermatan sulfate can be precisely and quantitatively controlled in the above invention, the separation or precise quantitative control of heparan sulfate and dermatan sulfate in heparan cannot be realized.

Method used

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  • A method for separating heparan sulfate and dermatan sulfate in heparin

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Embodiment 1

[0021] This embodiment provides a method for separating heparan sulfate and dermatan sulfate in heparan, which comprises the following steps:

[0022] (a) 40 g of heparinoid (specific optical rotation +5°, anticoagulant factor Xa activity 15 IU / mg) was dissolved in 400 ml of deionized water to prepare a 10% heparinoid solution;

[0023] (b) Inject the heparinoid solution into a polyacrylate anion exchange column with a column volume of 4 L for adsorption;

[0024] (c) Use 0~1mol / L sodium chloride solution for gradient elution (that is, linear gradient elution), the elution time is 100 minutes, and collect the eluent of each component; track with an ultraviolet detector (210nm ) to get as figure 1 The spectrogram shown;

[0025] (d) Concentrate the eluent of each component respectively (concentrate the eluent of each component to a sodium chloride concentration of 3~4wt%), add ethanol precipitation (so that each component elutes the ethanol in the mixed solution The concentr...

Embodiment 2

[0028] This example provides a method for separating heparan sulfate and dermatan sulfate in heparan, which is basically the same as the method in Example 1, except that: in step (a), a 5% heparan solution is prepared; finally 17 g of heparan sulfate and 17.5 g of dermatan sulfate were obtained.

Embodiment 3

[0030] This example provides a method for separating heparan sulfate and dermatan sulfate in heparan, which is basically the same as the method in Example 1, except that: in step (a), an 8% heparan solution is prepared; finally 17.2 g of heparan sulfate and 18.1 g of dermatan sulfate were obtained.

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Abstract

The present invention relates to a separation method of heparan sulfate and dermatan sulfate in heparin, which comprises the following steps: (a) dissolving heparin in water to prepare heparin solution; (b) passing the heparin solution through anion resin Adsorption; (c) Gradient elution using 0~1mol / L sodium chloride solution, and collecting the eluent of each component; (d) Concentrating the eluent of each component respectively, adding ethanol for precipitation to obtain sulfuric acid Heparan and dermatan sulfate. In this way, heparin-like by-products produced in the production of crude heparin to high-quality heparin can be used as raw materials, which avoids the loss of active ingredients in the prior art, does not introduce impurities, has simple process, low separation cost, good effect, and is easy to scale up.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a separation method, in particular to a separation method of heparan sulfate and dermatan sulfate in heparinoids. Background technique [0002] Heparin was first discovered in the liver and got its name. It is a mucopolysaccharide sulfate extracted from animals. It has anticoagulant and antithrombotic effects. It has been used to treat thrombosis since the 1930s. However, due to the different animal sources of heparin, there will be the possibility of species virus contamination, causing some problems when using heparin or low molecular weight heparin; at the same time, due to animal breeding and biochemical pollution during the extraction process of heparin, the heparin Applications are restricted to varying degrees. More than 40 years ago, when some European and American scientists headed by Lindahl and Choay were studying the anticoagulant and antithrombotic effects of heparin, they esta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10C08B37/08
CPCC08B37/0069C08B37/0075
Inventor 王芃
Owner SUZHOU WISMED PHARMA CO LTD
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