Kit for detecting staphylococcus capitis

Abstract

The invention provides a kit for detecting staphylococcus capitis. The kit comprises guide RNA specifically targeting staphylococcus capitis rpoB gene, an amplification primer pair, hydration TwistAmpbasic kit reaction drying balls, LbCas12a protein, a single-chain DNA probe (ssDNA), a Ribonuclease Inhibitor and a buffer solution. When the kit is used for detecting the staphylococcus capitis, high detection specificity is achieved, and the staphylococcus capitis can be distinguished from other staphylococcus such as staphylococcus aureus, staphylococcus hominis, staphylococcus warneri, staphylococcus epidermidis and staphylococcus haemolyticus. Meanwhile, when the RNA sequence is used to perform detection, detection time is about 1 hour, a PCR instrument is not needed, equipment requirements are evidently lower than those of a traditional bacterial culture method or a PCR sequencing method, and a high clinical practical value is achieved.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for the detection of Staphylococcus capitus, comprising a guide RNA specifically targeting the rpoB gene of Staphylococcus capitis, a pair of RPA amplification primers, and a hydrated TwistAmp basickit Reaction dry balls, LbCas12a protein, single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor, and buffer. This kit can be used for rapid detection of Staphylococcus capitis. Background technique [0002] Staphylococcus capitis is a common bacterial group among Gram-positive bacteria that infects humans, which brings a huge burden to patients and society. The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus capitis from other staphylococci due to the lack of specific primers, so joint generation sequencing is required It can only be identified, t...

AI Technical Summary

Problems solved by technology

The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus capitis from other staphylococci due to the lack of specific primers, so joint generation sequencing is required Can only be identified, the cost is high, and it takes several days to get the result

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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