Extraction technology of moringa flavonoid glycosides compound and application thereof in neck mask
A technology for the extraction of flavonol glycosides and flavonoids from Moringa oleifera, which is applied in the extraction process of flavonol glycosides from Moringa oleifera and its application in cervical membranes, can solve the problems of single function, weak cell metabolism, and easy elastic fatigue. Achieve and inhibit melanocytes, anti-inflammatory melanocytes, and improve neck skin quality
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Embodiment 1
[0034] A kind of extraction process of flavonol glycoside compound of Moringa oleifera flower, comprises the following steps:
[0035] Step (1), crush the dried Moringa oleifera flower, use 70% ethanol aqueous solution as the extraction solvent, extract at 60°C, filter to obtain the filtrate, and concentrate the filtrate under reduced pressure to obtain the Moringa oleifera flower ethanol extract ;
[0036] Step (2), dissolving the ethanol extract of Moringa oleifera flower obtained in step (1) in water at 40°C, extracting the obtained liquid with ethyl acetate, and taking the organic phase;
[0037] The mass ratio of the volume of water to the ethanol extract of Moringa oleifera flower is 2L: 1Kg;
[0038] In step (3), the organic phase obtained in step (2) is dehydrated and then concentrated to obtain extract A; after that, extract A is subjected to RP-18 medium-pressure reversed-phase silica gel column chromatography, and the organic solvent is mixed with petroleum ether a...
Embodiment 2
[0042] A kind of extraction process of flavonol glycoside compound of Moringa oleifera flower, comprises the following steps:
[0043] Step (1), crush the dried Moringa oleifera flowers to 30-50 meshes, use 72% ethanol aqueous solution as the extraction solvent, extract twice at 55°C, filter to obtain the filtrate, and concentrate the filtrate under reduced pressure. Obtain Moringa flower ethanol extract;
[0044]Step (2), dissolving the ethanol extract of Moringa oleifera flower obtained in step (1) in water at 60°C, extracting the obtained liquid with ethyl acetate, and taking the organic phase;
[0045] The mass ratio of the volume of water to the ethanol extract of Moringa oleifera flower is 3L: 1Kg;
[0046] In step (3), the organic phase obtained in step (2) is dehydrated and then concentrated to obtain extract A; after that, extract A is subjected to RP-18 medium-pressure reversed-phase silica gel column chromatography, and the organic solvent is mixed with petroleum e...
Embodiment 3
[0051] A kind of extraction process of flavonol glycoside compound of Moringa oleifera flower, comprises the following steps:
[0052] Step (1), after crushing the dried Moringa oleifera flowers to 30-50 meshes, using 75% ethanol aqueous solution as the extraction solvent, leaching three times at 50°C, filtering to obtain the filtrate, and concentrating the filtrate under reduced pressure to obtain Moringa flower ethanol extract; the mass ratio of the volume of ethanol aqueous solution to the dried Moringa flower is 20L: 1Kg during each extraction;
[0053] Step (2), dissolving the ethanol extract of Moringa oleifera flower obtained in step (1) in water at 50°C, extracting the obtained liquid with ethyl acetate, and taking the organic phase;
[0054] The mass ratio of the volume of water to the ethanol extract of Moringa oleifera flower is 2.5L: 1Kg;
[0055] In step (3), the organic phase obtained in step (2) is dehydrated and then concentrated to obtain extract A; after tha...
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