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Dry artificial biological heart valve containing polypeptide for promoting endothelialization and preparation method of dry artificial biological heart valve

A heart valve and endothelialization technology, applied in the field of medical materials, can solve the problems of difficult endothelialization, inability to quickly absorb water and flatten, and inability to preload, and achieve the effect of reducing additional risks, good performance of water immersion and flattening, and reducing residues

Active Publication Date: 2019-08-09
JILIN VENUS HAOYUE MEDICAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a dry artificial biological heart valve containing endothelialization-promoting polypeptide and its preparation method, which can effectively solve the problem that existing biological heart valves need to be stored in glutaraldehyde solution, cannot It is pre-loaded in the delivery system, requires temporary cleaning, presses the assembly, cannot quickly absorb water and flatten, and is difficult to endothelialize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A dry artificial heart valve containing endothelialization-promoting polypeptides, the preparation method of which comprises the following steps:

[0028] (1) cross-linking the cleaned porcine pericardium with 1% glutaraldehyde aqueous solution at room temperature and pH=7.4 for 24 hours;

[0029] (2) The porcine pericardium membrane after cross-linking in step (1) is cleaned with deionized water, then placed in poly(ethylene glycol) methacrylate aqueous solution (100mL) containing 5mg / mL RGD polypeptide, in Soaking at 25°C for 24 hours; wherein, the volume concentration of poly(ethylene glycol) methacrylate in the aqueous solution of poly(ethylene glycol) methacrylate is 10%;

[0030] (3) After cleaning the product of step (2) with deionized water, add 1wt% ammonium persulfate and 0.1wt% tetramethylethylenediamine and 0.1wt% N, N'-methylenebisacrylamide soaking in an aqueous solution (100 mL), the soaking temperature is 25° C., and the soaking time is 24 hours, then c...

Embodiment 2

[0032] A dry artificial heart valve containing endothelialization-promoting polypeptides, the preparation method of which comprises the following steps:

[0033] (1) cross-linking the cleaned porcine pericardium with 1% glutaraldehyde aqueous solution at room temperature and pH=7.4 for 24 hours;

[0034] (2) The porcine pericardium membrane after the cross-linking of step (1) was cleaned with deionized water, then placed in poly(ethylene glycol) methacrylate aqueous solution (100mL) containing 1mg / mLYIGSR polypeptide, at 25 Soak for 24 hours at ℃; wherein, the volume concentration of poly(ethylene glycol) methacrylate in the aqueous solution of poly(ethylene glycol) methacrylate is 20%;

[0035] (3) After cleaning the product of step (2) with deionized water, add 1wt% ammonium persulfate and 0.1wt% tetramethylethylenediamine and 0.1wt% N, N'-methylenebisacrylamide soaking in an aqueous solution (100 mL), the soaking temperature is 25° C., and the soaking time is 24 hours, the...

Embodiment 3

[0037] A dry artificial heart valve containing endothelialization-promoting polypeptides, the preparation method of which comprises the following steps:

[0038] (1) Cross-linking the cleaned porcine pericardium with 0.5% glutaraldehyde aqueous solution at room temperature and pH=7.4 for 48 hours;

[0039] (2) The porcine pericardium membrane after step (1) cross-linking is cleaned with deionized water, then placed in poly(ethylene glycol) methacrylate aqueous solution (100mL) containing 1mg / mLREDV polypeptide, at 25 Soak for 24 hours at ℃; wherein, the volume concentration of poly(ethylene glycol) methacrylate in the aqueous solution of poly(ethylene glycol) methacrylate is 20%;

[0040] (3) After cleaning the product of step (2) with deionized water, add 1wt% ammonium persulfate and 0.1wt% tetramethylethylenediamine and 0.1wt% N, N'-methylenebisacrylamide soaking in an aqueous solution (100 mL), the soaking temperature is 25° C., and the soaking time is 24 hours, then clean...

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Abstract

The invention discloses a dry artificial biological heart valve containing polypeptide for promoting endothelialization and a preparation method of the dry artificial biological heart valve. The method comprises the following steps that cross-linking is performed on amino groups and carboxyl groups of an animal pericardium, and endothelialization treatment, hydrogel polymerization treatment and dewatering and drying treatment are performed on the animal pericardium, wherein the endothelialization treatment involves that the polypeptide for promoting the endothelialization is adopted to treat the animal pericardium. The preparation method has the advantages that the prepared dry biological heart valve has good water immersion flattening performance, and can be quickly immersed in water andflattened at a higher temperature and higher humidity, thereby reducing residues of glutaraldehyde and the like, and reducing the problems of calcification and toxicity which are caused by the glutaraldehyde and the like, therefore the preoperative installation of a valve system can be simplified, and the additional risks of surgeries are reduced; the polypeptide capable of promoting the endothelialization is also added in the preparation process, so that the prepared biological valve has the property of promoting the endothelialization.

Description

technical field [0001] The invention belongs to the technical field of medical materials, in particular to a dry artificial biological heart valve containing endothelialization-promoting polypeptide and a preparation method thereof. Background technique [0002] Heart valve disease is a common disease of valve degeneration, which is anatomically manifested by narrowing of blood pathways or valve insufficiency. Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. [0003] Biological heart valves refer to a class of biomedical materials used to replace human diseased heart valves. Biological heart valves are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/22A61L27/54A61L27/16A61L27/52C08J3/24C08F283/06C08F222/38C08L89/00
CPCA61L27/16A61L27/227A61L27/3604A61L27/3687A61L27/52A61L27/54A61L2300/252A61L2300/414A61L2430/20A61L2430/40C08F283/065C08J3/24C08J2389/00C08L71/02C08L89/00C08F222/385
Inventor 雷洋王云兵
Owner JILIN VENUS HAOYUE MEDICAL LTD
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