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Dry artificial biological heart valve containing growth factor for promoting endothelialization and preparation method of dry artificial biological heart valve

A technology of growth factors and heart valves, applied in the field of dry artificial heart valves and its preparation, can solve problems such as difficult endothelialization, inability to quickly absorb water to flatten, and inability to preload, etc., to reduce additional risks and achieve good water immersion flattening performance, residue reduction effect

Active Publication Date: 2019-08-09
JILIN VENUS HAOYUE MEDICAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a dry artificial biological heart valve containing endothelialization-promoting growth factors and a preparation method thereof, which can effectively solve the problem that existing biological heart valves need to be stored in glutaraldehyde solution, It cannot be pre-loaded in the delivery system, and it needs to be temporarily cleaned during use. The pressure grip assembly cannot quickly absorb water and flatten, and it is difficult to endothelialize

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] A dry artificial biological heart valve containing endothelialization-promoting growth factors, the preparation method of which comprises the following steps:

[0027] (1) cross-linking the cleaned porcine pericardium with 1% glutaraldehyde aqueous solution at room temperature and pH=7.4 for 24 hours;

[0028] (2) The porcine pericardium membrane cross-linked in step (1) was cleaned with deionized water, and then placed in 2-methacryloyloxyethyl phosphorylcholine aqueous solution (100 mL) containing 5 μg / mL VEGF, in Soaking at 25°C for 24 hours; wherein, the volume concentration of 2-methacryloyloxyethylphosphorylcholine in the aqueous solution of 2-methacryloyloxyethylphosphorylcholine is 10%;

[0029] (3) After cleaning the resultant of step (2) with deionized water, add 1wt% ammonium persulfate and 0.1wt% tetramethylethylenediamine and 0.1wt% N, N'-methylenebisacrylamide soaking in an aqueous solution (100 mL), the soaking temperature is 25° C., and the soaking time...

Embodiment 2

[0031] A dry artificial biological heart valve containing endothelialization-promoting growth factors, the preparation method of which comprises the following steps:

[0032] (1) cross-linking the cleaned porcine pericardium with 1% glutaraldehyde aqueous solution at room temperature and pH=7.4 for 24 hours;

[0033] (2) Wash the porcine pericardium cross-linked in step (1) with deionized water, and then place it in an aqueous solution of 2-methacryloyloxyethyl phosphorylcholine (100 mL) containing 1 μg / mL SDF-1α , soaking at 25°C for 24 hours; wherein, the volume concentration of 2-methacryloyloxyethylphosphorylcholine in the aqueous solution of 2-methacryloyloxyethylphosphorylcholine is 20%;

[0034] (3) After cleaning the resultant of step (2) with deionized water, add 1wt% ammonium persulfate and 0.1wt% tetramethylethylenediamine and 0.1wt% N, N'-methylenebisacrylamide Soak in an aqueous solution (100 mL), the soaking temperature is 25° C., and the soaking time is 24 hour...

Embodiment 3

[0036] A dry artificial biological heart valve containing endothelialization-promoting growth factors, the preparation method of which comprises the following steps:

[0037] (1) Cross-linking the cleaned porcine pericardium with 0.5% glutaraldehyde aqueous solution at room temperature and pH=7.4 for 48 hours;

[0038] (2) Wash the porcine pericardium cross-linked in step (1) with deionized water, and then place it in an aqueous solution of 2-methacryloyloxyethyl phosphorylcholine (100 mL) containing 1 μg / mL LTGF-β1 , soaking at 25°C for 24 hours; wherein, the volume concentration of 2-methacryloyloxyethylphosphorylcholine in the aqueous solution of 2-methacryloyloxyethylphosphorylcholine is 30%;

[0039](3) After cleaning the resultant of step (2) with deionized water, add 1wt% ammonium persulfate and 0.1wt% tetramethylethylenediamine and 0.1wt% N, N'-methylenebisacrylamide Soak in an aqueous solution (100 mL), the soaking temperature is 25° C., and the soaking time is 24 ho...

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Abstract

The invention discloses a dry artificial biological heart valve containing a growth factor for promoting endothelialization and a preparation method of the dry artificial biological heart valve. The method comprises the following steps that cross-linking is performed on amino groups and carboxyl groups of an animal pericardium, and endothelialization treatment, hydrogel polymerization treatment and dewatering and drying treatment are performed on the animal pericardium, wherein the endothelialization treatment involves that the growth factor for promoting the endothelialization is adopted to treat the animal pericardium. The preparation method has the advantages that the prepared dry biological heart valve has good water immersion flattening performance, and can be quickly immersed in water and flattened at a higher temperature and higher humidity, thereby reducing residues of glutaraldehyde and the like, and reducing the problems of calcification and toxicity which are caused by the glutaraldehyde and the like, therefore the preoperative installation of a valve system can be simplified, and the additional risks of surgeries are reduced; the growth factor capable of promoting the endothelialization is also added in the preparation process, so that the prepared biological valve has the property of promoting the endothelialization.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a dry artificial biological heart valve containing endothelialization-promoting growth factors and a preparation method thereof. Background technique [0002] Heart valve disease is a common disease of valve degeneration, which is anatomically manifested by narrowing of blood pathways or valve insufficiency. Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. [0003] Biological heart valves refer to a class of biomedical materials used to replace human diseased heart valves. Biological h...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/54A61L27/16C08F230/02C08F222/38C08F128/02C08J3/075C08L43/02C08L41/00
CPCA61L27/16A61L27/227A61L27/3604A61L27/3687A61L27/54A61L2300/252A61L2300/414A61L2430/20A61L2430/40C08F128/02C08F230/02C08J3/075C08J2341/00C08J2343/02C08L43/02C08L41/00C08L89/00C08F222/385
Inventor 王云兵雷洋
Owner JILIN VENUS HAOYUE MEDICAL LTD
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