CAD gene sequence related to synthesis of oenanthe javanica lignin and its application

A gene, cress technology, applied to a CAD gene of a plant and its application field, can solve problems affecting taste and so on

Inactive Publication Date: 2019-08-06
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But in vegetables, after the lignin content increases, the plant will become lignified, which will greatly affect the taste

Method used

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  • CAD gene sequence related to synthesis of oenanthe javanica lignin and its application
  • CAD gene sequence related to synthesis of oenanthe javanica lignin and its application
  • CAD gene sequence related to synthesis of oenanthe javanica lignin and its application

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Embodiment Construction

[0011] 1. Extraction of cress total RNA and synthesis of cDNA: the cress sample was extracted with RNA Simple Total RNA Kit total RNA extraction kit (Beijing Tiangen Company), and extracted according to Prime Script RT reagent Kit instructions Cress total RNA was reverse transcribed into cDNA.

[0012] 2. Cloning of the cress OjCAD gene: According to the cress transcriptome data of our research group (Jiang Q et al., Molecular Genetics & Genomics, 2015, 290(2):671-683.), the cress OjCAD gene was retrieved sequence, and designed a pair of cloning primers, forward primer sequence: 5'-ATGACGCAAACAACTCCCCAA-3', reverse primer sequence: 5'-CTATGGTTTTCCAGCAATGT-3'. PCR amplification was carried out using watercress cDNA as a template. The total volume of the amplification system is 10.0 μL, including 0.30 ng·μL-1cDNA template 0.5 μL, 2×TaKaRa Ex Taq Mix (product number RR001A) 5.0 μL, ddH2O 3.5 μL, 0.10 μmol L -1 0.5 μL each of forward primer and reverse primer. The amplification...

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Abstract

The CAD gene is a key gene involved in lignin synthesis. The present invention clones a new CAD gene OjCAD from oenanthe javanica. An open reading frame of the gene is 1074 bp in length, and the geneencodes 357 amino acids. The OjCAD-encoded protein is hydrophobin with a typical CAD1 conserved domain, which belongs to the MDR family. The protein has the closest relationship with the evolution ofApiaceae carrot CAD, and is highly conservative. Fluorescence quantitative expression analysis indicates that the expression level of the OjCAD gene in Baguazhoushuiqin is higher than that of Nanxuanbaguazhouzishuiqin, and the expression level in the leaves is significantly higher than the petiole. The invention uses a polymerase amplification technology to clone to obtain the oenanthe javanica OjCAD gene from the oenanthe javanica, and the oenanthe javanica OjCAD gene has an effect on the synthesis of lignin in the oenanthe javanica.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a plant CAD gene and its application. The present invention obtains a gene OjCAD related to lignin synthesis from the cress variety 'Baguazhou cress', and this gene can be applied to the research and application of the lignin synthesis pathway of cress. Background technique [0002] Cress [Oenanthe javanica (Blume) DC.] is a perennial herb belonging to the genus Cress of Umbelliferae, and is an important aquatic vegetable. Cress is both edible and medicinal. In addition to being rich in essential nutrients for the human body, cress is also rich in anti-vitamins, dietary fiber, apigenin, ascorbic acid, flavonoids and other ingredients, which can lower blood pressure and calm the nerves, and enhance the body's immunity to the external environment , prevention of cardiovascular and cerebrovascular diseases, has high medicinal value and health care function. [0003] Lignin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/04C12N15/53C12N15/10
CPCC12N9/0006C12Q1/6895C12Q2600/158C12Y101/01195
Inventor 熊爱生仇亮王枫
Owner NANJING AGRICULTURAL UNIVERSITY
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