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Method for measuring contents of alkaloids, lignans and nucleosides in isatis root or preparation thereof by using quantitative analysis single-maker method

A technology of lignans and alkaloids, which is applied in the direction of measuring devices, material separation, and analysis of materials, to achieve the effects of saving detection costs and time, improving work efficiency, and high sensitivity

Active Publication Date: 2019-08-02
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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  • Claims
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Problems solved by technology

[0006] At present, there is no research report on the simultaneous determination of the content of alkaloids, nucleosides and lignans in Radix isatidis or its preparations by the one-test and multiple-evaluation method

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  • Method for measuring contents of alkaloids, lignans and nucleosides in isatis root or preparation thereof by using quantitative analysis single-maker method
  • Method for measuring contents of alkaloids, lignans and nucleosides in isatis root or preparation thereof by using quantitative analysis single-maker method
  • Method for measuring contents of alkaloids, lignans and nucleosides in isatis root or preparation thereof by using quantitative analysis single-maker method

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Embodiment Construction

[0040] The following is a further description of the present invention, rather than a limitation of the present invention.

[0041] Instruments and reagents

[0042]Instrument: Agilent 1260 high-performance liquid chromatography (Agilent, USA); Thermo UltiMate 3000 high-performance liquid chromatography (Thermo Fisher, USA); Waters2695-2996 high-performance liquid chromatography (Waters, USA); column Agilent ZORBAX-C 18 (250mm×4.6mm, 5μm); Column Waters Xbridge-C 18 (250mm×4.6mm, 5μm); Chromatographic column Phenomenex Kinetex-C 18 (250mm×4.6mm, 5μm); CPA 225D one-hundred-thousandth balance (Shanghai Precision Instrument Co., Ltd.), KQ-600VDB dual-frequency CNC ultrasonic cleaner (Shanghai Shengke Instrument Equipment Co., Ltd.).

[0043] Reagent: (R,S)-Gaoyichun reference substance (batch number: 111753-201706, for content determination, with a purity of 100wt%, provided by China Institute for Food and Drug Control), cytidine (batch number: B20073, for content determinatio...

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Abstract

The invention discloses a method for measuring the contents of alkaloids, lignans and nucleosides in the isatis root or preparation thereof by using a quantitative analysis single-maker method. With low-cost stable-structure avaible alkaloids compound (R,S)-epigoitrin as a reference, relative correction factors with lignans compound clemastanin B and nucleoside compounds cytidine, uridine, vernine, and adenosine are established. Therefore, the contents of another five kinds of lignan and nucleoside contents with high content rates are calculated by using the correction factors only by measuring the content of the(R,S)-epigoitrin of the isatis root or preparation, thereby carrying out multi-indicator quality evaluation on the isatis root or preparation simply, conveniently, comprehensivelyand accurately. The quality controllability and curative effect stability of the product can be guaranteed; and the detection cost and time can be saved.

Description

[0001] Technical field: [0002] The invention relates to the technical field of isatidis root detection, in particular to a method for determining the contents of alkaloids, lignans and nucleosides in isatidis root or its preparations by using a one-test and multiple-evaluation method. [0003] Background technique: [0004] Radix Isatidis is the dried root of the cruciferous plant Jianglan. Its functions and indications are clearing heat and detoxifying, cooling blood and relieving sore throat. It is used for febrile epidemic disease, fever, sore throat, febrile toxin rash, mumps, rotten throat Dansha, big head plague, erysipelas, and carbuncle. Radix isatidis mainly contains lignans, alkaloids, nucleosides and other active ingredients. Lignan is a kind of plant secondary metabolite formed by oxidative polymerization of 2 molecules of phenylpropanoid derivatives. It has various pharmacological activities and important application values ​​such as anti-virus, anti-inflammati...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 黄远董福越王德勤李淑如李楚源
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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