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Simple urine sugar quantitative detection method and urine sugar quantitative detection kit

A quantitative detection method and urine sugar technology, which are applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of low data accuracy and inability to obtain urine sugar content, big error

Active Publication Date: 2019-08-02
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the determination of urine sugar content is mainly qualitative analysis methods represented by Benedictine reagent method and urine sugar test paper method. Due to its complicated operation and low data accuracy, Benedictine reagent method has been gradually eliminated and its usage rate is getting lower and lower. ; and although the commercial test paper method is simple to operate, it is still based on the naked eye qualitative determination of the color card, which has a large error and is easily affected by factors such as the test paper brand, the time the test paper is immersed in urine, and the colorimetric time. A rough qualitative result without being able to get a precise urine sugar content
In view of this, scientists have carried out some studies on the quantitative analysis of urine sugar, but these methods not only require superb technology to prepare complex analytical materials, but also require specific analytical equipment such as fluorospectrophotometer, chemiluminescence apparatus, etc., which are not suitable for routine analysis, especially patient self-monitoring

Method used

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  • Simple urine sugar quantitative detection method and urine sugar quantitative detection kit
  • Simple urine sugar quantitative detection method and urine sugar quantitative detection kit
  • Simple urine sugar quantitative detection method and urine sugar quantitative detection kit

Examples

Experimental program
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Effect test

Embodiment

[0047] This embodiment provides a simple quantitative detection method for urine sugar, and uses this method to detect the glucose content in the urine of diabetic patients.

[0048] a. The urine of 4 diabetic patients (half male and half female) was collected, and all samples were used fresh without pretreatment and dilution.

[0049] b. Add 30uL TMB (2mg / mL), 30uL HRP (100ng / mL), 10uL H 2 O 2 (0.078%), 130uL substrate buffer solution, 50ul GOD (10mg / mL, GOD≥300U / g), to obtain four blue test solutions.

[0050] c. Mix the 4 parts of the urine to be tested collected in step a with the four parts of the blue test solution obtained in step b, shake gently for 10 seconds to mix well, and take pictures after standing for 10 minutes, and read the blue color of each colorimetric solution image. Color intensity value (that is, B value in RGB color).

[0051] Since there is a linear relationship between the logarithm value (i.e. lnC) of the glucose concentration (C) in the solution...

experiment example

[0053] The method provided by the present invention will be described below in conjunction with experimental data.

[0054] 1. Methods and materials:

[0055] 1. Instruments and test methods

[0056] Place the microtiter plate flat on a white background, place the Xiaomi 6X smartphone vertically 20cm above the microtiter plate, turn off the flash, aim the camera at each well of the microtiter plate, take pictures and collect images in the automatic mode, and finally Analyze the RGB value of the image through the software package Color Picker 1.5.2 in the mobile phone

[0057] 2. Materials

[0058] Glucose Oxidase (Glucose Oxidase, GOD≥300U / g), horseradish peroxidase (horseradishperoxidsae, HRP), 3,3'5,5'-tetramethylbenzidine (3,3'5,5'- tetramethylbenzidine, TMB) was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai). Substrate buffer solution (prepared as 1.84g Na 2 HPO 4 , 1.84g of citric acid added with water to 100mL), hydrogen peroxide (H 2 O 2 ), p...

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Abstract

The invention provides a simple urine sugar quantitative detection method and a urine sugar quantitative detection kit and belongs to the field of biological detection. The urine sugar quantitative detection method comprises the following steps: mixing 3, 3', 5, 5'-tetramethyl benzidine, horse radish peroxidase, hydrogen peroxide solution and glucose oxidase to obtain a blue detection solution; uniformly mixing the blue detection solution and urine to be tested to obtain a colorimetric solution; measuring intensity value of blue in the colorimetric solution; and calculating blood sugar value in the urine to be tested. The method is low in detection cost, high in accuracy and convenient and fast, does not depend on specific analytical instruments and is especially suitable for daily detection of diabetes patients at home.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a simple urine sugar quantitative detection method and a urine sugar quantitative detection kit. Background technique [0002] Diabetes is called "emerging global epidemic" by WHO, and its incidence rate is increasing year by year, which has become a serious global public health problem. The content of glucose in blood or urine is an important index for clinical diagnosis of diabetes doctors and daily self-monitoring of patients. Since the blood glucose test needs to draw blood from a vein or fingertip after skin disinfection, the troublesome disinfection process and the stinging pain when taking blood make the patient's compliance very poor. Therefore, urine glucose measurement with non-invasive detection features is receiving more and more attention in patients' daily self-monitoring. [0003] At present, the determination of urine sugar content is mainly qualitative analy...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 青琳森王甜甜张志锋罗培梁健
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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