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Application of Juniper plant polysaccharide in preparing medicine for preventing and treating viral acute lung injury

A technology of acute lung injury and plant polysaccharides, applied in the preparation of drugs for the prevention and treatment of viral acute lung injury, new drug application field of plant polysaccharides of Cupressaceae Juniper

Active Publication Date: 2022-01-18
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Throughout the research at home and abroad, there is no research report on the treatment of viral acute lung injury with polysaccharides from Juniper genus

Method used

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  • Application of Juniper plant polysaccharide in preparing medicine for preventing and treating viral acute lung injury
  • Application of Juniper plant polysaccharide in preparing medicine for preventing and treating viral acute lung injury
  • Application of Juniper plant polysaccharide in preparing medicine for preventing and treating viral acute lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Cedar take medicine 100g, pulverized, cold soak in 95% ethanol extracted three times, dried dregs home ventilation at room temperature and then extracted 3 times with hot water, filtered, and the combined extracts were concentrated and ethanol was added to a solution of alcohol an amount of 80%, the precipitate reconstituted in order to free protein trichloroacetic acid, centrifuged, the supernatant was dialyzed against water for 3 days, the dialysate was concentrated and freeze-dried to obtain the total polysaccharide. Sulfuric acid - phenol as measured cedar Polysaccharide content 81.2%; inter-hydroxybiphenyl as measured using the Cedar polysaccharides uronic acid content of 11.1%; protein using Coomassie brilliant blue as measured cedar polysaccharide content 1.1 %.

Embodiment 2

[0039] BALB / C 36 mice (14-16g), were randomly divided into 6 groups (N, M, A, B, C, P): N is the normal group, M is a group H1N1 virus model group, A is a group 7.5mg / kg, group B polysaccharide cedar cedar polysaccharide 15mg / kg, group C polysaccharide cedar 30mg / kg, P group, positive drug ribavirin 100mg / kg, n = 6, all animals prop tail vein injection of propofol anesthesia, N intranasally 1640 30μL group as a control; the other group intranasal infection 5LD 50 H1N1 virus solution 30 L, two hours after the oral administration H1N1 infection, N group and M group given 0.5% CMC oral administration, and as a normal viral control, administered once a day for consecutive four days, H1N1 viruses 96h after weighing the weight, eyeball blood carefully cut the whole lung, blotted dry blood filter paper, weighing records; carefully cut on the lobe in 10% formalin; middle right lung, the left lung and the lower lobe rinse with saline, was placed stored at -80 ℃. Lung tissue after...

Embodiment 3

[0046] BALB / C mice (14-16 g), which were randomly divided into 6 groups (N, M, A, B, C, P): N group as a normal control group, and the M group was H1N1 viral model group, Group A For the cedar polysaccharide group 7.5mg / kg, Group B is 15mg / kg of Cyber ​​polysaccharide group, c group is 30mg / kg / kg of Cyber ​​polysaccharide group, p group is 100mg / kg of Libelin, 10 in each group; Animal is anesthetized by propofol, and the N group drops of 1640 medium was 30 μl as a control; dripped infection 10LD 50 H1N1 virus 30 μL, A, and P group infected H1N1 after 2 hours, the N group was administered 0.5% CMC gas in the N group, as normal and viral control, administration once a day, seven days of continuous administration, stop The drug was observed in 14 days, and the amount of animals and the number of deaths were recorded daily, and the drug protection rate of the drug on severe flu infection with pneumonia mice was calculated;

[0047] The results show that after oral cork pol...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and in particular relates to the use of polysaccharides from Juniper genus plants in the preparation of medicines for preventing and treating viral acute lung injury. The present invention separates and extracts the total polysaccharides from juniper plants Cedarwood and Dianzi cypress, and the sugar content exceeds 60%. It has been confirmed by overall experiments that the polysaccharide has a significant therapeutic effect on the acute lung injury induced by influenza A virus H1N1 in mice, can inhibit the replication of influenza virus in the mouse lung, reduce the capillary permeability of the lung, relieve Pulmonary hemorrhage and pulmonary edema, inhibition of systemic and local inflammatory responses in the lungs, and significantly improving the survival rate of mice infected with severe influenza virus; the polysaccharides of the Juniper plant can be further used to prepare drugs for preventing and treating viral acute lung injury.

Description

Technical field [0001] The present invention belongs to the technical field of Chinese medicine, relates to new pharmaceutical uses Cupressaceae Sabina plant polysaccharides. Sabina particularly to the use of plant polysaccharides in preventing and treating acute lung injury viral medicament. Background technique [0002] Studies have reported that viral lung damage from a variety of acute lung injury is caused by a viral infection, usually caused by viral upper respiratory tract infection caused by the spread down. Studies have shown that acute lung injury caused by a virus influenza virus is common, also it includes parainfluenza virus, cytomegalovirus, adenovirus, rhinovirus, coronavirus and so on. Studies have reported that influenza A virus is an important pathogen causing human and avian respiratory diseases, viruses cause bronchiolitis bronchial epithelium, infection spread among pulmonary interstitial and alveolar pneumonia caused; studies have shown that, on the one hand...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P11/00A61P31/16A61P31/14A61P31/22A61K36/14
CPCA61K31/715A61K36/14A61K2236/331A61K2236/39A61K2236/53A61K2236/51A61K2236/333
Inventor 卢燕陈道峰傅泽龙力弘
Owner FUDAN UNIV
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