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A kind of preparation method of ratiometric broad-spectrum photoelectric immunosensor

An immunosensor and broad-spectrum technology, which is applied in the preparation of ratiometric broad-spectrum photoelectric immunosensors, can solve problems such as food safety accidents and underestimation of total ochratoxin intake.

Active Publication Date: 2021-04-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, if OTA is only detected, the total intake of ochratoxin will be underestimated, which will easily cause food safety accidents

Method used

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  • A kind of preparation method of ratiometric broad-spectrum photoelectric immunosensor
  • A kind of preparation method of ratiometric broad-spectrum photoelectric immunosensor
  • A kind of preparation method of ratiometric broad-spectrum photoelectric immunosensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing a ratiometric broad-spectrum photoelectric immunosensor, comprising the following steps:

[0039] S1. Synthesis of CdS / MoS on FTO 2 Nanocomposites: synthesis of sheet-like MoS on FTO conductive glass 2 Nanosheets, get MoS 2 Nanosheet-modified FTO, followed by MoS 2 CdS nanoparticles loaded on nanosheets to obtain CdS / MoS 2 Nanocomposite modified FTO;

[0040] The synthesis of flake MoS on FTO conductive glass 2 The specific operation of the nanosheet is: the Na 2 MoO 4 2H 2 O and L-cysteine ​​were added to the mixed solution of water and ammonia water to obtain mixed solution A; FTO and mixed solution A were added to the reactor, after hydrothermal reaction, washed and dried to obtain modified MoS 2 FTO of nanosheets.

[0041] described in MoS 2 The specific operation of loading CdS nanoparticles on the nanosheets is as follows: the CdCl 2 , NH 4 Add Cl, thiourea, and ammonia water to distilled water and heat to obtain solution B; then...

Embodiment 2

[0050] Example 2 CdS / MoS 2 Nanocomposite Characterization

[0051] CdS / MoS 2 Synthesis of Nanocomposites:

[0052] (1) Synthesis of flake MoS on FTO conductive glass 2 . 45 mg of Na 2 MoO 4 2H 2 The L-cysteine ​​of 0 and 100mg joins in the mixed solution of the water of 15ml and ammoniacal liquor, and the volume ratio of control middle water and ammoniacal liquor is 2:1. Then, put the FTO into the reactor, and add the above solution into the reactor, and conduct a hydrothermal reaction at 200° C. for 24 hours. After the reaction, remove the MoS 2 Nanosheet-modified FTO, washed and dried.

[0053] (2) in MoS 2 CdS nanoparticles loaded on the nanosheets. 0.0013mol CdCl 2 , 0.0076mol NH 4 Add Cl, 0.18mol thiourea, and 25.84mL ammonia water to 500ml distilled water and heat to 80°C, then add MoS 2 -FTO was added and reacted for 5 minutes. Remove CdS / MoS after completion 2 Nanocomposite modified FTO, washed and dried.

[0054] MoS 2 The results of the SEM images o...

Embodiment 3

[0056] Example 3 ZnS-Ag 2 S@Ab 2 Nanomaterial Characterization

[0057] ZnS-Ag 2 S@Ab 2 multi-target synthesis

[0058] (1) Synthesis of ZnS-Ag 2 S nanomaterials. 0.023g Zn(Ac) 2 and 0.03g of L-cysteine ​​were added to 15ml of ethylene glycol / water mixed solution with a volume ratio of 1:4, and heated at 150°C for 12 hours. Wash by centrifugation and disperse in 20ml of methanol solution. 20 μL concentration of 5g / LAgNO 3 The solution was added dropwise to the methanol solution of ZnS, stirred, washed by centrifugation and dispersed in 5ml of water.

[0059] (2) Secondary antibody in ZnS-Ag 2 Modifications on S. In 2ml ZnS-Ag 2 To the S solution, add 0.2 mL of thioglycolic acid and stir at room temperature for 12 hours. After washing by centrifugation, disperse in 1 mL of EDC / NHS solution (20 mg·mL -1 / 10mg·mL -1 ) for 1 hour. After washing by centrifugation again, disperse in 1ml of phosphate buffer solution (PBS, pH 7.4, 0.01M), add 10 microliters of 1mg / ml se...

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Abstract

The invention discloses a method for preparing a ratiometric broad-spectrum photoelectric immunosensor. Firstly, flake MoS is prepared. 2 material, increase the surface area of ​​the substrate, and then control the time in MoS by chemical deposition 2 CdS nanoparticles were deposited on it as a photoelectrode to achieve a stable photocurrent under visible light. Second, a three-dimensional ZnS‑Ag 2 S nanoflowers to immobilize secondary antibodies, not only Ag 2 Good energy level pairing between S and CdS can reduce the change signal of photocurrent, and HCl can release Zn after material treatment 2+ , can generate electrochemical differential pulse signal. The method of the present invention overcomes the uncertainty of single-signal detection, and creatively constructs a dual-signal immunosensor, that is, the photoelectric signal response of the photoelectric material and the electrical signal response of the electrochemical probe, which can realize simultaneous detection of three ochratoxins. Inspection, and can effectively reduce background noise and improve detection accuracy.

Description

technical field [0001] The invention relates to the technical field of sensors, in particular to a preparation method of a ratiometric broad-spectrum photoelectric immunosensor. Background technique [0002] The signal detection of immunosensors is an important guarantee of sensitivity, and the common method is single signal amplification based on light, electricity, photoelectricity, mass, etc. However, whether "signal off" or "signal on" single-signal detection has uncertainties, such as batch-to-batch differences in substrate material morphology and bioburden; chemical reactions involved in the detection itself have false positives, etc. [0003] Food safety is related to public health and life safety. my country is a country dominated by agricultural production, and crops may be polluted by mycotoxins during growth, storage and transportation, causing huge economic losses. Ochratoxins (Ochratoxins) are another mycotoxin that has attracted worldwide attention after afla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48G01N33/543
CPCG01N27/305G01N27/3278G01N27/48G01N33/54346
Inventor 刘英菊梁弘志敖日其冷谢玄张悦
Owner SOUTH CHINA AGRI UNIV
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