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Biological film type preserving fluid and preparation method thereof

A preservation solution and biofilm technology, which can be used in the preservation, application, and animal husbandry of human or animal bodies, and can solve problems such as limited effects, damage to cells and biomolecules, etc.

Inactive Publication Date: 2019-07-12
广州锐澄医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ionizing radiation is gamma rays or high-energy electron beams, which can cause damage to cells and biomolecules at the same time
Conventional means to reduce adverse effects is to lower the temperature, or even irradiate in a frozen state, but the effect is still limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In a class 100 clean environment, add 1 g of low molecular weight dextran to 500 ml of commercially available MEM cell culture medium, add 100 mg of penicillin and 100 mg of streptomycin, add 0.5 g of vitamin C, and fully dissolve the above substances evenly at room temperature. Then add 500ml of glycerol and mix well. Cover the mouth of the container with filter paper, transfer to an autoclave, and autoclave at 140°C for 30 minutes. After cooling down to room temperature, seal and refrigerate for future use.

Embodiment 2

[0027] In a class 100 clean environment, take a pack of MEM cell culture medium dry powder (configure 500ml), pour it into a sterilized 500ml container, add 400ml sterile water, wash the inner surface of the packaging bag twice with sterile water and pour it into the container middle. Stir to completely dissolve the dry powder evenly. Add 1.1 g of sodium bicarbonate, dissolve evenly, and adjust the pH to 7.2 with HCL or NaOH. The volume of the solution was then adjusted to 500 ml with sterile water. Subsequently, 0.2 g of hyaluronic acid with a molecular weight of 300w was added and fully dissolved. Add 150 mg of penicillin and 150 mg of streptomycin, and add 0.2 g of proanthocyanidins to fully dissolve them evenly. Then add 500ml of 1,3-propanediol and mix well. Cover the mouth of the container with filter paper, transfer to an autoclave, and autoclave at 140°C for 30 minutes. After cooling down to room temperature, seal and refrigerate for future use.

Embodiment 3

[0029] In a class 100 clean environment, add 0.4g of hyaluronic acid with a molecular weight of 150w to 500ml of commercially available MEM cell culture medium, add 100mg of penicillin and 100mg of streptomycin, add 0.4g of glutathione, and make the above substances fully dissolved evenly. Add 200ml of glycerol and 300ml of 1,3-propanediol and mix well. Cover the mouth of the container with filter paper, transfer to an autoclave, and autoclave at 140°C for 30 minutes. After cooling down to room temperature, seal and refrigerate for future use.

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Abstract

The invention provides a biological film type preserving fluid. Every 1 L of the preserving fluid is prepared from the following components: 300-700 ml of an MEM cell culture liquid, 0-5 g of low molecular weight dextran, 0-2 g of hyaluronic acid with the molecular weight of 5-300 w and a double-antibody containing 20-200 mg / L of penicillin and streptomycin. In addition, every 1 L of the preserving fluid contains 300-700l ml of glycerine and / or 1,3-propylene glycol and or one or more of 0-10 g / L cysteine, 0-10 g / L vitamin C, 0-1g / L procyanidine and 0-3 g / L glutathione. The preserving fluid hasthree functions of protecting active ingredients in a biological film in an irradiation sterilization deactivating process, protecting the structure of the biological film in freezing and preservingperiods and protecting the biological function of the biological film in the preserving period. The invention also provides a preparation method of the biological film type preserving fluid which is convenient to prepare, good in economical benefit and clear in function, covers a production process and a preserving stage of the biological film and is suitable for long-term preservation of a wet biological film in a frozen state.

Description

technical field [0001] The invention relates to the technical field of biological agent preparations, in particular to a biofilm preservation solution and a preparation method thereof. Background technique [0002] Biofilms usually contain many active components and even living cells, so they have good efficacy in clinical use. In order to maintain good biological efficacy, certain preservation methods and preservation reagents are necessary. At present, most active preservation methods for biofilms are wet preservation. The collection and processing of animal-derived biofilms involves multiple processes. Usually, the place and time of implementation of these processes are not consistent, and the purpose of process treatment is also different. It is necessary to consider the role of the protective solution in multiple situations: such as protection during product handling and transfer, protection during freezing treatment, and protection during irradiation treatment. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 王涛杨习锋曾晨光王晓然王柏文
Owner 广州锐澄医疗技术有限公司
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