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Application of dendrobium polysaccharide in preparation of drugs for prevention and/or treatment of benign prostatic hyperplasia

A technology for prostatic hyperplasia and Dendrobium polysaccharide, which is applied in the biological field to achieve the effects of wide source of raw materials, improved antioxidant capacity and good application prospects

Active Publication Date: 2019-07-09
中科众康(广东)生物科技研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the water-soluble polysaccharides of Dendrobium officinale have preventive and recovery effects on benign prostatic hyperplasia, whether its adverse effects on spermatogenesis and function caused by benign prostatic hyperplasia, and whether the polysaccharides of Dendrobium candidum can maintain the stability of the prostate tissue physiological environment and improve its antioxidant capacity. However, it is still blank whether the prevention and adjuvant treatment of benign prostatic hyperplasia have medicinal effects

Method used

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  • Application of dendrobium polysaccharide in preparation of drugs for prevention and/or treatment of benign prostatic hyperplasia
  • Application of dendrobium polysaccharide in preparation of drugs for prevention and/or treatment of benign prostatic hyperplasia
  • Application of dendrobium polysaccharide in preparation of drugs for prevention and/or treatment of benign prostatic hyperplasia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The extraction of embodiment 1 Dendrobium officinale polysaccharide

[0056] 1. Preparation of Dendrobium polysaccharide water extract

[0057] Weigh 4g of Dendrobium officinale powder (purchased from the Crop Research Institute of the Guangdong Academy of Agricultural Sciences), add 10 times the volume of distilled water, fully dissolve, and bathe in a water bath at 90°C for 100 minutes, stirring once every 30 minutes during this period, and filter with gauze to obtain filtrate. Repeat the above process 3 times, combine the filtrates, concentrate with a freeze dryer, add 4 times the volume of absolute ethanol, precipitate with alcohol at 4°C for 12 hours and centrifuge to obtain a precipitate, then wash the precipitate with 80% ethanol for 2 to 3 times, at 40°C Dried to obtain the crude polysaccharide powder of Dendrobium officinale.

[0058] 2. Determination of polysaccharide content

[0059] Weigh 100mg of anhydrous glucose, place it in a dry 100mL volumetric flas...

Embodiment 2

[0061] Embodiment 2 animal experiments

[0062] 1. Solution preparation

[0063] (1) Testosterone propionate injection (25g / L), purchased from Ningbo Second Hormone Factory (batch number 110251054).

[0064] (2) Application solution of testosterone propionate injection: use soybean oil as a solvent to dilute the testosterone propionate injection to 0.625 mg / mL, and use it as a solution for subcutaneous injection in mice.

[0065] (3) preparation of dendrobium officinale polysaccharide solution:

[0066] 1 g of the crude polysaccharide powder of Dendrobium officinale prepared in Example 1 was dissolved in 100 mL of distilled water, and shaken up to prepare a 10 mg / mL crude polysaccharide solution of Dendrobium officinale.

[0067] 2. Animal experiment steps

[0068] 5-week-old, healthy male Kunming mice weighing 25 ± 2 g were purchased from the Guangdong Provincial Experimental Animal Center, and the experiment was started after 1 week of adaptation. The mice were bred acco...

Embodiment 3

[0078] The detection of embodiment 3 mouse prostate and testis coefficient

[0079] The isolated mouse prostate and testis were weighed respectively, and the prostate coefficient and testis coefficient were calculated according to the following formula:

[0080] Organ index = organ wet weight mg / mouse body weight g

[0081] Table 1 Effect of DOP on mouse prostate coefficient (unit: mg / g)

[0082]

[0083]

[0084] Table 2 The effect of DOP on the testis coefficient of mice (unit: mg / g)

[0085]

[0086] The data is n=10. "a" means there is a significant difference compared with the blank control group at the same treatment time (P21 Compared with the testosterone propionate group, there was a significant difference (P21 Compared with the testosterone propionate+D. officinale polysaccharide group, there was a significant difference (P<0.05).

[0087] Depend on figure 1 , figure 2 As can be seen from Table 1 and Table 2, compared with the same period of control mic...

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Abstract

The invention discloses an application of dendrobium polysaccharide in the preparation of drugs for prevention and / or treatment of benign prostatic hyperplasia. The dendrobium polysaccharide has obvious therapeutic effect and no toxic and side effects, which can improve the antioxidant capacity of prostate and testes, restore normal expression of key genes of steroid synthesis in leydig cells of benign prostatic hyperplasis, inhibit the expression of 5 alpha-reductase gene in the benign prostatic hyperplasia tissue and effectively treats benign prostatic hyperplasia, especially benign prostatic hyperplasia induced by exogenous androgen. The dendrobium polysaccharide with wide source of raw materials is simple in extraction and purification method, high in sugar content and suitable for large-scale production and popularization and has a good prospect in the preparation and / or treatment of prostate hyperplasia.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the application of dendrobium water-soluble polysaccharides in the preparation of drugs for preventing and / or treating benign prostatic hyperplasia. Background technique [0002] Benign prostatic hyperplasia (BPH) is the most common degenerative disease among middle-aged and elderly men. Among them, the prevalence rate of men over 40 years old is about 50%, and almost all men over 60 years old suffer from BPH in varying degrees. There are many theories about the pathogenesis of the disease, such as the theory of dihydrotestosterone, the theory of estrogen and androgen synergy, etc., but the pathogenesis is not completely clear. It is generally accepted that androgen plays an important role in the pathogenesis of BPH. Androgen testosterone (Testosterone) is a steroid hormone, which is synthesized by male testes or female ovaries. It plays an important role in male spermatog...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P13/08
CPCA61K31/715
Inventor 芦春斌仇平乐李春梦姚炜炜朱倍倍
Owner 中科众康(广东)生物科技研究院
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