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A kind of bone repair material and its application

A technology for bone repair and chondroitin sulfate, applied in the fields of medical biomaterials and medical devices, can solve problems such as poor mechanical properties and strong immunogenicity, and achieve the effects of low storage conditions, convenient storage, and significant bone defect repair effects.

Active Publication Date: 2021-09-21
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of strong immunogenicity and poor mechanical properties in the prior art, the present invention provides a bone repair material

Method used

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  • A kind of bone repair material and its application
  • A kind of bone repair material and its application
  • A kind of bone repair material and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Evaluation of the composition and composition of the bone powder prepared as a raw material

[0049] Choose bone sample (ionic skeleton) via a strong base, strong oxidizing agent, hot water soaked, degreasing, deprotein, decell, dehydrated, calcined by high temperature, inactivating possible viruses, abrasive, sieve, regulate pH To neutral, get natural bone powder.

[0050] 1) The varying skeleton is derived from a healthy cattle under the age of 3 years old, cutting the bone block with no more than 2 cm, rinsing the blood on the bone block.

[0051] 2) The alkalization purpose is to degreasing, decellular, formulated with an alkali: 1M NaOH solution.

[0052] One alkalization: the ratio of the mass volume of the bone block and the alkali solution is 1: 2, soaked for 24 h.

[0053] Induction: peeling the impurities on the bone block.

[0054] Secondary alkalization: the ratio of the mass volume of the bone block and the alkali solution is 1: 2, soaked for 24 h.

...

Embodiment 2

[0067] Example 2: Preparation of Bone Repair Materials

[0068] 1. Preparation of natural hydroxyapatite: Natural hydroxyapatite (natural bone powder) was prepared according to the method of Example 1;

[0069] 2. Preparation of collagen gel:

[0070] 1) Slowly add 500 ml of chondroitin acetic acid solution to 500 ml of collagen acetic acid swelling liquid, the stirring speed is 15,000-20000 rpm, the stirring time is 1-2 hours, the solution is evacuated, resulting in collagen-sulfate chondroitin Slurry;

[0071] Substitridic acid solution configuration:

[0072] 2-4 grams of sulfuric acid chondroitin was added to a 500 ml concentration of 0.4 to 0.6% acetic acid solution, dissolved under temperature conditions at 0-10 ° C, and filtered.

[0073] Collagen acetic acid swelling liquid configuration:

[0074] 3-5 g of type I collagen was added to a 500 ml concentration of 0.3 0.6% acetic acid solution, mixed with stirring, the stirring speed was 15,000-200,000 rpm, the stirring temper...

Embodiment 3

[0087] Example 3: Analysis of the morphology and ingredients of bone repair materials and bone flour

[0088] Example 2 was prepared by a scanning electron microscope (SEM, S4800, HATICHI), and elements distribution analysis were performed by an incidental EDS.

[0089] image 3 For the SEM photo of the bone repair material, it is possible to see that the phosphate particles in the bone repair material are less than 250 μm, and the collagen adhesion is formed, and the collagen is distributed between the collagen and the phosphate particles, the collagen is a smooth film, while the phosphate particles are further The enlargement can be observed that a large amount of 500 nm of hydroxyapatite crystals, and the nanocrystals form a micro-nano-stage hole structure. This structure retains from natural bone microstructure, so that the bone repair material has water absorption and the adsorption of proteins such as growth factor. effect. Further analysis of the bone repair materials such ...

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Abstract

The invention provides a bone repair material. The molecular structure and calcium-phosphorus content ratio of the bone repair material are very similar to those of natural bone, and have excellent biocompatibility. When the bone repair material is implanted into a bone defect, there is no fibrous tissue interface between the bone tissue and the hydroxyapatite in the repair material, and the bone-like apatite mineral material can be formed on the surface after implantation in the body, and dynamically participate in bone tissue absorption And reconstruction and calcium ion, phosphorus ion metabolic process. At the same time, the bone repair material of the present invention has excellent osteoconductivity and osteoinductivity, and the three-dimensional structure allows blood vessel growth, cell penetration and attachment, new bone formation, tissue deposition and calcification, and the bionic microstructure is beneficial to bone morphology Aggregation of protein (BMP) occurs and osteoinductivity occurs.

Description

Technical field [0001] The present invention belongs to medical biomaterials, medical devices, particularly, to a bone repair material, preparation method and application. Background technique [0002] For a long time, mainly bone defect repair method of bone allograft binding autogenous bone graft. Autologous bone osteoinductive higher, non-immunogenic, but limited sources of autologous bone. There are problems caused allograft immune response, and may cause cross-infection. These materials are of biological origin inevitable defects, synthetic or natural materials has become a promising alternative to bone defect repair material. [0003] Inducible kind of good bone substitute should have the following characteristics: ① good biocompatibility and biodegradability; ② bone conduction and together with other active molecular complexes induce osteogenesis; ③ can load a large number of cells, supports the growth of bone cells and differentiation; ④ with a suitable shaping of the mec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/58A61L27/56
CPCA61L27/46A61L27/56A61L27/58A61L2430/02C08L89/00
Inventor 陈刚郭晓霖
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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