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Detection method for polycyclic aromatic hydrocarbons in edible oil

A polycyclic aromatic hydrocarbon and detection method technology, which is applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve problems such as the limitation of detection objects, affect the detection limit, and limit the wide application, and achieve good SERS stability. Sensitivity, lower detection limit, and the effect of large SERS enhancement

Active Publication Date: 2019-06-28
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the size of the cavity can only detect PAH molecules that match its volume, the detection object has certain limitations.
However, the physical adsorption method adsorbs fewer intermediary molecules, which will lead to a corresponding decrease in target molecules, which will eventually affect its detection limit.
In addition, the modification of SERS substrates requires delicate professional operations, which limits its wide application.

Method used

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  • Detection method for polycyclic aromatic hydrocarbons in edible oil
  • Detection method for polycyclic aromatic hydrocarbons in edible oil
  • Detection method for polycyclic aromatic hydrocarbons in edible oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] In this embodiment, the Raman parameters are set as: excitation wavelength 785 nm, laser power 8%, integration time 5 s, and accumulation times 1 time.

[0045] Add benzopyrene to chloroform, ultrasonically shake for 5 minutes to completely dissolve benzopyrene in chloroform, and prepare a sample with a concentration of benzopyrene of 0.1 ppm. Take 1mL of this sample and 4mL of gold nano sol, mix and shake vigorously for 30s to form a three-dimensional plasma liquid ball. Test results such as image 3 shown.

Embodiment 2

[0051] In this embodiment, the Raman parameters are set as: excitation wavelength 785 nm, laser power 8%, integration time 5 s, and accumulation times 1 time.

[0052] Take 1mL of chloroform containing 80ppb, 60ppb, 40ppb, 20ppb, 10ppb and 0ppb of benzopyrene in the container vial of hydrophilic treatment, respectively, and 4mL of gold nanosol with a diameter of 100nm (at this time, the enhancement of SERS The effect is the best), shake vigorously to assemble into a three-dimensional plasma liquid ball, and then transfer it to a cuvette for Raman detection, such as Figure 4 shown. The Raman characteristic peaks of benzopyrene are: 609cm -1 、1233cm -1 and 1379cm -1 . Choose 609cm -1 The Raman peak at 663cm -1 The chloroform peak at is used as an internal standard, and the result is R 2 = 0.94.

Embodiment 3

[0054] In this embodiment, the Raman parameters are set as: excitation wavelength 785 nm, laser power 8%, integration time 5 s, and accumulation times 1 time.

[0055] Add benzopyrene to corn oil and ultrasonically shake for 5 minutes to completely dissolve benzopyrene in corn oil to prepare samples with benzopyrene concentrations of 0ppb, 10ppb, 20ppb, 40ppb, 80ppb and 100ppb, and then prepare samples containing benzopyrene The preparation process is as follows: (1) Take 4 mL of corn oil containing benzopyrene and 2 mL of acetonitrile, vibrate vigorously and ultrasonically intermittently, each for 2 min; (2) centrifuge at 10,000 r / min for 2 min to separate the analyte Extract solution; (3) Take 300 μL of the sample extract, 700 μL of chloroform and 4 mL of gold nano-sol in a centrifuge tube, shake vigorously for 30 seconds to form a three-dimensional plasma liquid ball (4) add 300 μL of the sample extract to the system , Vigorously shaken for 30s, this process was repeated 3 ...

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Abstract

The invention discloses a detection method for polycyclic aromatic hydrocarbons in edible oil. The method comprises the following steps that the edible oil is added into an extractant, and layered after violent oscillation, and an extraction layer includes a polycyclic aromatic hydrocarbons extract; a precious metal nanometer sol is uniformly mixed with an organic solvent whose density is higher than that of water to form a mixed solution; the polycyclic aromatic hydrocarbons extract is added to the mixed solution, and shocked violently to form a 3D plasma liquid sphere; the above steps are repeated, part volume is removed from the total organic phase slowly by sucking, and the 3D plasma liquid sphere is condensed in-situ; and Raman detection is carried out on the 3D plasma liquid sphere after in-situ condensation. According to the detection method, polycyclic aromatic hydrocarbons can be detected needless of modifying a nanometer material, the method is simple and convenient in operation, and a related device is portable and can be used to detect the polycyclic aromatic hydrocarbons in edible oil onsite.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a detection method for polycyclic aromatic hydrocarbons in edible oil. Background technique [0002] When edible oil is fried at high temperature for a long time (generally 150-200°C), complex chemical reactions such as hydrolysis, thermal oxidation, thermal polymerization, thermal cracking, isomerization, and cyclization will occur, and various harmful substances will be generated. Products, such as polycyclic aromatic hydrocarbons. Polycyclic aromatic hydrocarbons (PAHs) is a general term for hydrocarbons and their derivatives that contain two or more benzene rings and are connected by fused rings. PAHs are a class of extremely inert hydrocarbon compounds with stable chemical properties and widely exist in various environmental media, mainly from the pyrolysis or incomplete combustion of petroleum, coal tar, tobacco and other organic substances. Therefore, in The pla...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/38
Inventor 刘洪林苏梦可蒋伊帆于烦烦
Owner HEFEI UNIV OF TECH
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