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Method for measuring soil microbial biomass carbon and nitrogen content

A technology of soil microorganisms and biomass, applied in bioinformatics, color/spectral characteristic measurement, instruments, etc., to achieve the effect of accurate and reliable conversion coefficient, high safety and simple operation

Inactive Publication Date: 2019-06-21
LANZHOU UNIVERSITY
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Problems solved by technology

[0006] However, how to determine the relationship between soil microbial DNA content and soil microbial biomass carbon and nitrogen, and how to determine the conversion coefficient of microbial carbon and nitrogen content have not yet been clarified.

Method used

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  • Method for measuring soil microbial biomass carbon and nitrogen content
  • Method for measuring soil microbial biomass carbon and nitrogen content
  • Method for measuring soil microbial biomass carbon and nitrogen content

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Effect test

Embodiment 1

[0041] Step 1: Collecting Microorganisms

[0042] A total of 138 soil samples of 5 main terrestrial ecosystems including desert, farmland, grassland, meadow and forest were collected. These soil samples were further subdivided into 25 different microhabitats according to the differences in their habitats. The specific terrestrial ecosystems and The microhabitat is:

[0043] Desert: under the canopy, bare land;

[0044] Farmland: corn high density 900 plants / m 2 , Corn medium density 100 plants / m 2 , corn low density 1 plant / m 2 , Soybean high density 900 plants / m 2 , Soybean medium density 100 plants / m 2 , Soybean low density 1 plant / m 2 , Weed plots;

[0045] grassland;

[0046] Meadow: flat land, fertilization land, sunny slope, slope land, grazing forbidden wetland, grazing wetland;

[0047] Forest: 0-20cm, 20-40cm, 40-60cm at an altitude of 2245 meters, 0-20cm, 20-40cm, 40-60cm at an altitude of 2416 meters, 0-20cm at an altitude of 2587 meters , 20-40cm, 40-60cm...

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Abstract

The invention relates to a method for measuring soil microbial biomass carbon and nitrogen content, comprising the steps of: extracting the soil microbial DNA in a soil sample to be measured; measuring the concentration of the extracted DNA by using a micro spectrophotometer and converting the concentration into a unit mass soil microbial DNA content; and separately multiplying the soil microbialDNA content by a microbial biomass carbon content conversion coefficient and a microbial biomass nitrogen content conversion coefficient, to calculate the soil microbial biomass carbon content and thesoil microbial biomass nitrogen content. The method of the invention can accurately estimate the soil microbial biomass carbon and nitrogen content just by extracting the microbial DNA from a small number of soil samples, omits a chloroform fumigation step, is accurate and reliable in conversion coefficient, improves the measurement efficiency of soil microbial biomass carbon and nitrogen content, and is relatively easy and safe to operate, and high in safety.

Description

technical field [0001] The invention relates to a method for measuring the carbon and nitrogen of microbial biomass in soil. Specifically, a new method for measuring the carbon and nitrogen content of soil microbial biomass is designed by combining and utilizing the existing soil DNA extraction technology and means. Background technique [0002] Soil microbial biomass (Mibrobial Biomass, MB) refers to those in the soil whose volume is less than 5×10 3 μm 3 The total biomass is the active part of soil organic matter, but living plants are not included, such as living plant roots, and some large animals should not be included. Soil microbial biomass in a broad sense includes microbial biomass carbon, nitrogen, phosphorus, and sulfur, but it is generally considered that soil microbial biomass only refers to the microbial biomass carbon content in soil. As an active part of the ecosystem, soil microorganisms perform important functions. On the one hand, soil microorganisms are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G16B20/00
Inventor 邓建明龚海洋胡维刚杜洽军侯晴晴
Owner LANZHOU UNIVERSITY
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