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Preparation method and application of 3D chip for screening of traditional Chinese medicine components

A traditional Chinese medicine component and three-dimensional chip technology, which is applied in chemical instruments and methods, analytical materials, laboratory containers, etc., can solve the problems of low drug small molecule immobilization capacity and poor specificity of small molecule array chips, etc., and achieve improved The effect of fixed amount, guaranteed uniformity, and simple preparation method

Active Publication Date: 2019-06-21
QIANNAN NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when the small molecule array chip technology is used for high-throughput screening of traditional Chinese medicine components containing different small molecule structures, the low immobilization capacity of small drug molecules and the poor specificity of the small molecule array chip have become the technical bottlenecks.

Method used

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  • Preparation method and application of 3D chip for screening of traditional Chinese medicine components
  • Preparation method and application of 3D chip for screening of traditional Chinese medicine components
  • Preparation method and application of 3D chip for screening of traditional Chinese medicine components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Add deionized water, 25% ammonia water and 30% hydrogen peroxide into the chip cleaner, where the volume ratio of deionized water, ammonia water and hydrogen peroxide is 5:1:1, and then put it on the surface For the glass substrate coated with gold film, the temperature was raised to 50°C to clean the substrate for 30 minutes, then rinsed with deionized water and ethanol, dried with nitrogen, and the cleaning was completed;

[0047] (2) Soak the cleaned substrate obtained in step (1) in 1mM 2-mercaptoethylamine ethanol solution, and place it at room temperature for 15 hours to self-assemble a monomolecular layer on the surface of the substrate, and then assemble the surface with 2 -The substrate of mercaptoethylamine monolayer was soaked in N,N-dimethylformamide (DMF) solution containing triethylamine and 2-bromopropionyl bromide, reacted at -5°C for 3 hours, and then React at room temperature for 8 hours, then clean the chip to obtain a substrate with an initiator ...

Embodiment 2

[0050] (1) Add deionized water, 25% ammonia water and 30% hydrogen peroxide into the chip cleaner, where the volume ratio of deionized water, ammonia water and hydrogen peroxide is 5:1:1, and then put it on the surface For the glass substrate coated with gold film, the temperature was raised to 70°C to clean the substrate for 10 minutes, then rinsed with deionized water and ethanol, dried with nitrogen, and the cleaning was completed;

[0051] (2) Soak the cleaned substrate obtained in step (1) in 5mM 2-mercaptoethylamine ethanol solution, and place it at room temperature for 10 hours to self-assemble a monomolecular layer on the surface of the substrate, and then assemble the surface with 2 -The substrate of mercaptoethylamine monolayer was soaked in the N,N-dimethylformamide (DMF) solution containing triethylamine and 2-bromopropionyl bromide, and reacted at 5°C for 2 hours, then at room temperature The reaction was carried out for 5 hours, and then the chip was cleaned to o...

Embodiment 3

[0054] (1) Add deionized water, 25% ammonia water and 30% hydrogen peroxide into the chip cleaner, where the volume ratio of deionized water, ammonia water and hydrogen peroxide is 5:1:1, and then put it on the surface For the glass substrate coated with gold film, the temperature was raised to 60°C to clean the substrate for 20 minutes, then rinsed with deionized water and ethanol, dried with nitrogen, and the cleaning was completed;

[0055] (2) Soak the cleaned substrate obtained in step (1) in 2mM 2-mercaptoethylamine ethanol solution, and place it at room temperature for 12 hours to self-assemble a monomolecular layer on the surface of the substrate, and then assemble the surface with 2 -The substrate of mercaptoethylamine monolayer was soaked in the N,N-dimethylformamide (DMF) solution containing triethylamine and 2-bromopropionyl bromide, reacted at 0°C for 2.5 hours, and then at room temperature The reaction was carried out for 6 hours, and then the chip was cleaned to...

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Abstract

The invention discloses a preparation method and an application of a 3D chip for screening of traditional Chinese medicine components. The chip comprises a support layer, an initiator layer and a polymer brush layer, wherein the initiator layer is anchored to the support layer through metal-sulfur bond covalence; the polymer brush layer is covalently bound on the initiator layer. The 3D chip for screening of traditional Chinese medicine components can fix drug micromolecules of different structures or traditional Chinese medicine components; besides, the chip is of a 3D structure, strong anti-nonspecific monomers are introduced into the polymer layer, so that the 3D chip for screening of the traditional Chinese medicine components greatly improves the fixation quantity of drug micromolecules or traditional Chinese medicine components, notably enhances the sensing signals and solves the problem about poor specificity of micromolecular array chips.

Description

technical field [0001] The invention relates to the field of biochip preparation, in particular to a preparation method and application of a three-dimensional chip for screening components of traditional Chinese medicine. Background technique [0002] Now to clarify the active ingredients of drugs, we must use modern drug screening technology. Drug screening is to apply appropriate methods and techniques to natural products or synthetic compounds that may be used as drugs to detect their possible pharmacological activities. Applying the latest technology of modern drug screening to the research of traditional Chinese medicine will greatly promote the modernization of traditional Chinese medicine research, such as high-throughput drug screening technology. Today, high-throughput screening technology has become the core technology for theoretical research on structures and drug development by pharmaceutical companies, and small molecule array chips are the most widely used. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/552
Inventor 彭开美戴学新杨再波黄德娜杨艳毛海立邹洪涛
Owner QIANNAN NORMAL UNIV FOR NATTIES
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