Optical flow control cell sorting chip and method

Abstract

The invention discloses an optical flow control cell sorting chip and method. The chip comprises a substrate, a microfluidic channel and an optical waveguide. The microfluidic channel is disposed in the substrate, and the optical waveguide is movably disposed on the substrate. The microfluidic channel comprises at least a first channel and a second channel which are mutually communicated, the first channel and the second channel are both used for accommodating microfluidics, and the first channel is further used for accommodating at least two kinds of cells. The optical waveguide comprises anoptical signal input end and a capture end. The optical signal input end is used for accessing an optical signal, the capture end is used for adsorbing one of at least two kinds of cells, and the capture end is used for releasing the adsorbed cells to the second channel. The chip is simple in structure and manufacturing method, low in cost, capable of sorting micro-nano cells accurately efficiently and flexibly by using the cone waveguide and capable of separating unmarked biological cells.

Description

technical field [0001] The invention belongs to the field of optofluidic technology, and in particular relates to an optofluidic cell sorting chip and a method for sorting cells thereof. Background technique [0002] Cell sorting is the basis of biological research and clinical diagnosis, and plays a vital role in the fields of single cell analysis, disease detection and diagnosis. Traditional cell sorting methods mainly include fluorescence activated cell sorting, dielectrophoresis sorting and magnetophoretic sorting. However, the above methods require additional biochemical markers, large sample volumes, high electric and magnetic fields, etc., which greatly limit the further application of cell sorting in the isolation of label-free, rare living cells. In 1987, Ashkin et al. proposed in "Science" that optical tweezers can manipulate micro / nano particles or living cells in fluid media without physical contact by focusing light beams. However, traditional optical tweezers...

AI Technical Summary

Problems solved by technology

However, traditional optical tweezers have several shortcomings: First, the excitation equipment is large and complex, expensive and has a large loss

Therefore, the preparation and maintenance of the experimental platform require high economic and time costs

Second, it cannot be flexibly controlled, and it is difficult to combine with microfluidic chips

Fiber optic tweezers have the advantages of simple preparation, small size, and flexible operation, but usually only have a single function, that is, to capture or repel particles / cells, and it is difficult to manipulate and separate two different cells at the same time.

Images